Analysis of the transcriptome of bovine endometrial cells isolated by laser micro-dissection (1): specific signatures of stromal, glandular and luminal epithelial cells

Chankeaw, Wiruntita; Lignier, Sandra; Richard, Christophe; Ntallaris, Theodoros; Raliou, Mariam; Guo, Yongkang; Plassard, Damien; Bevilacqua, Claudia; Sandra, Olivier; Andersson, Göran; Humblot, P.; Charpigny, Gilles

doi:10.1186/s12864-021-07712-0

Cited by 13 publications

(19 citation statements)

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“…The PCA from full RNA-seq data corroborates previous work [ 13 ] documenting that LE, GE, and ST cells of the bovine endometrium exhibit different molecular signatures. The full description of the characteristics of the transcriptome of the different cell types is beyond the scope of this article (lists are set in Supplementary File 4 ).…”

Section: Discussionsupporting

confidence: 87%

“…This study confirms the difficulty in capturing samples with good quality RNA from LE. As in Chankeaw et al [ 13 , 14 ], despite preparation of a high number of slides from biopsies, the number of sequenced samples was lower for LE than for GE and ST cells.…”

Section: Discussionmentioning

confidence: 82%

“…The overrepresented processes in ST cells are more numerous and diverse than in GE and LE cells. Most of them relate to regulatory processes, which is consistent with the ST regulatory role exerted on adjacent epithelial cells in co-culture systems [ 35 ], or within the cow endometrium [ 13 ]. This regulatory role of ST cells also emerges from the overrepresentation of cell communication and signalling processes, which are paramount for the coordination of cellular responses.…”

Section: Discussionmentioning

confidence: 84%

“…The expression of genes encoding proteins related to microvilli adhesion and assembly support that bovine endometrial GE cells form a cluster of tightly packed microvilli, as observed in rat endometrial GE cells [ 24 ]. As example, low density lipoprotein receptor class A domain containing 1 is a membrane receptor already identified in GE cells in previous work [ 13 ] and also expressed by mature ciliated cells in airway epithelium [ 25 ]. Cow endometrial GE cells have specialized functions regarding synthesis, transport and secretion of substances into the uterine lumen [ 26 ], here illustrated by the overrepresentation of biological processes of regulation of peptide secretion and cilium movement, the latter believed to be essential for moving secretory products across the surface of GE cells into the uterine lumen [ 27 ].…”

Section: Discussionmentioning

confidence: 99%

“…This method has been used to study the interactions between the endometrial epithelial and stromal compartments with seminal plasma in a murine model [ 9 ] and the conceptus induced regulation of endometrial function in the porcine [ 10 ], ovine [ 11 ] and equine [ 12 ] species. In the cow, recent studies based on this approach described the specific molecular signatures of endometrial stromal, glandular and luminal epithelial cells, as well as the effect of negative energy balance on the transcriptomic profiles of endometrial compartments in the mid-luteal phase [ 13 , 14 ]. The above experiments revealed that the different endometrial cell types show distinct transcription patterns.…”

Section: Introductionmentioning

confidence: 99%

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Progesterone differentially affects the transcriptomic profiles of cow endometrial cell types

et al. 2022

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Background The endometrium is a heterogeneous tissue composed of luminal epithelial (LE), glandular epithelial (GE), and stromal cells (ST), experiencing progesterone regulated dynamic changes during the estrous cycle. In the cow, this regulation at the transcriptomic level was only evaluated in the whole tissue. This study describes specific gene expression in the three types of cells isolated from endometrial biopsies following laser capture microdissection and the transcriptome changes induced by progesterone in GE and ST cells. Results Endometrial LE, GE, and ST cells show specific transcriptomic profiles. Most of the differentially expressed genes (DEGs) in response to progesterone are cell type-specific (96%). Genes involved in cell cycle and nuclear division are under-expressed in the presence of progesterone in GE, highlighting the anti-proliferative action of progesterone in epithelial cells. Elevated progesterone concentrations are also associated with the under-expression of estrogen receptor 1 (ESR1) in GE and oxytocin receptor (OXTR) in GE and ST cells. In ST cells, transcription factors such as SOX17 and FOXA2, known to regulate uterine epithelial-stromal cross-talk conveying to endometrial receptivity, are over-expressed under progesterone influence. Conclusions The results from this study show that progesterone regulates endometrial function in a cell type-specific way, which is independent of the expression of its main receptor PGR. These novel insights into uterine physiology present the cell compartment as the physiological unit rather than the whole tissue.

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Section: Discussionsupporting

confidence: 87%

Section: Discussionmentioning

confidence: 82%

Section: Discussionmentioning

confidence: 84%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Progesterone differentially affects the transcriptomic profiles of cow endometrial cell types

et al. 2022

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The effect of obesity on uterine receptivity is mediated by endometrial extracellular vesicles that control human endometrial stromal cell decidualization and trophoblast invasion

Galio,

Bernet,

Rodriguez

et al. 2023

J of Extracellular Bio

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The objectives of the present study were to determine whether obesity impacts human decidualization and the endometrial control of trophoblast invasion (both of which are required for embryo implantation) and evaluate the potential involvement of endometrial extracellular vesicles (EVs) in the regulation of these physiological processes. Using primary human cell cultures, we first demonstrated that obesity is associated with significantly lower in vitro decidualization of endometrial stromal cells (ESCs). We then showed that a trophoblastic cell line's invasive ability was greater in the presence of conditioned media from cultures of ESCs from obese women. The results of functional assays indicated that supplementation of the culture medium with EVs from nonobese women can rescue (at least in part) the defect in in vitro decidualization described in ESCs from obese women. Furthermore, exposure to endometrial EVs from obese women (vs. nonobese women) was associated with significantly greater invasive activity by HTR‐8/SVneo cells. Using mass‐spectrometry‐based quantitative proteomics, we found that EVs isolated from uterine supernatants of biopsies from obese women (vs. nonobese women) presented a molecular signature focused on cell remodelling and angiogenesis. The proteomics analysis revealed two differentially expressed proteins (fibronectin and angiotensin‐converting enzyme) that might be involved specifically in the rescue of the decidualization capacity in ESCs from obese women; both of these proteins are abundantly present in endometrial EVs from nonobese women, and both are involved in the decidualization process. In conclusion, our results provided new insights into the endometrial EVs’ pivotal role in the poor uterine receptivity observed in obese women.

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Generation of a novel three-dimensional scaffold-based model of the bovine endometrium

et al. 2023

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Bovine in vitro endometrial models that resemble tissue function in vivo are needed to study infertility, long-term uterine alterations induced by pathogens and impact of endocrine disruptor chemicals on reproductive function and other reproductive system complications that cause high economic losses in livestock species. The present study aimed to generate an innovative, reproducible, and functional 3D scaffold-based model of the bovine endometrium structurally robust for long term-culture. We developed a multicellular model containing both endometrial epithelial and stromal cells. Epithelial cells organized to form a luminal-like epithelial layer on the surface of the scaffold. Stromal cells produced their own extracellular matrix forming a stable subepithelial compartment that physiologically resembles the normal endometrium. Both cell types released prostaglandin E2 and prostaglandin F2α following a treatment with oxytocin and arachidonic acid. Additionally signal pathways mediating oxytocin and arachidonic acid stimulation of prostaglandin synthesis were analyzed by real time PCR (RT-PCR). Oxytocin receptor (OXTR), prostaglandin E2 receptor 2 (EP2), prostaglandin E2 receptor 4 (EP4), prostaglandin F receptor (PTGFR), prostaglandin E synthase (PTGES), PGF-synthase (PGFS) and prostaglandin-endoperoxide synthase 2 (COX-2) expression was detected in both control and treatment groups, however, only significant changes in abundance of OXTR mRNA transcripts were found. The results obtained by this study are a step forward in bovine in vitro culture technology. This 3D scaffold-based model provides a platform to study regulatory mechanisms involved in endometrial physiology and can set the basis for a broader tool for designing and testing novel therapeutic strategies for recurrent uterine pathologies.

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Analysis of the transcriptome of bovine endometrial cells isolated by laser micro-dissection (1): specific signatures of stromal, glandular and luminal epithelial cells

Cited by 13 publications

References 90 publications

Progesterone differentially affects the transcriptomic profiles of cow endometrial cell types

Progesterone differentially affects the transcriptomic profiles of cow endometrial cell types

The effect of obesity on uterine receptivity is mediated by endometrial extracellular vesicles that control human endometrial stromal cell decidualization and trophoblast invasion

Generation of a novel three-dimensional scaffold-based model of the bovine endometrium

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