Analysis of the treg cell population in the peripheral blood of ovarian cancer patients in relation to the long-term outcomes

Abstract

Objectives: There is growing evidence that Treg cell infiltration into the cancer nest is associated with poor prognosis. However, the Treg cell population in the peripheral blood may change when a different type of anticancer therapy is applied. Since Treg cells may support tumor growth by enhancing the suppressive profile of the cancer microenvironment, the assessment of Treg cells can bring to light important information regarding prognosis. Thus we decided to analyze the Treg cell population in the peripheral blood in relation to long-term outcomes in the group of patients with ovarian cancer. Material and methods:The 80 patients included in the study were treated surgically followed by chemiotherapy for ovarian cancer between October 2010 through May 2011.The peripheral blood samples from the patients were collected directly prior to chemotherapy. Information on any patients who died was retrieved from the database of the Cuiavia-Pomerania Regional Office of the National Health System of Poland. CD4+CD25+FOXP3+ lymphocytes T were assed by flow cytometry. We have analyzed the long term outcomes of treatment regarding to the level of Treg cells in peripheral blood. Results:We found that patients with serous adenocarcinomas had significantly higher Treg levels compared to those patients with non-serous types. Patients who had a higher percentage of Treg cells within the CD4+ cell population prior to the beginning of the treatment had worse long-term outcomes from the applied therapy. Conclusions:The assessment of Treg levels prior to the start of chemotherapy is clinically useful and may predict overall survival in ovarian cancer patients.

Role of exosomes in the immune microenvironment of ovarian cancer (Review)

“…VEGF-induced MDSCs inhibit the activity of CD8 + T cells in ovarian cancer, weakening the host's antitumor immune response and leading to a poor prognosis (54). A study showed that a higher count of Treg cells could be detected in the peripheral blood of patients with ovarian cancer and thus is associated with poor prognosis (55).…”

“…Tregs negatively regulate the antitumor response in both a direct and indirect manner while also playing a key role in immune escape (162). The increase of Tregs in the tumor microenvironment of patients is related to poor prognosis and shortened overall survival (OS) time (55). Treg-derived exosomes can exert immunosuppressive effects by expressing CD73 and inhibiting the proliferation of CD4 + T cells (107).…”

Role of CD25 expression on prognosis of acute myeloid leukemia: A literature review and meta-analysis

“…Liu et al suggested that higher percent of circulating CD4+CD25+CD127 T cells can predict OS and chemotherapeutic response in patients with unresectable pancreatic cancer [27]. Dutsch-Wicherek et al also found that patients with serous adenocarcinomas had significantly CD25+ lymphocytes T levels compared to those patients with non-serous types [26]. There analyses identified CD25 may be attributable to the plasticity of T cell subsets.…”

“…This meta-analysis indicated that CD25 overexpression is an indicator of poor prognosis for patients with AML. Where the majority of research had focused on hematological malignancies [ 25 ], there have been studies paying attention to the prognostic value of CD25 in solid tumors [ 26 – 28 ]. Liu et al suggested that higher percent of circulating CD4+CD25+CD127 T cells can predict OS and chemotherapeutic response in patients with unresectable pancreatic cancer [ 27 ].…”

Mice Treated with an Antibiotic Cocktail Containing Metronidazole Show Attenuated Allergy Responses through the Induction of Regulatory T Cells in the Intestinal Mucosa

“…A Zombie red Fixable Viability Kit BioLegend was used to exclude dead cells, according to the manufacturer s protocol. Following the staining of surface antigens, intracellular Foxp3 staining was performed using a Transcription Factor Buffer set BD Biosciences 13 . For intracellular IL-10 staining, a single cell suspension was incubated with LPS 10 µg/ml , PMA 50 ng/ml , ionomycin 500 ng/ml , and Brefeldin A 1 µg/ml for 5 h at 37ºC followed by intracellular IL-10 staining performed using a Transcription Factor Buffer set 14 .…”