Analysis of the uptake of atmospheric ammonia by leaves of Phaseolus vulgaris L.

Hove, L.W.A. van; Koops, Andries J.; Adema, E.H.; Vredenberg, W.J.; Pieters, G.A.

doi:10.1016/0004-6981(87)90115-6

Cited by 67 publications

(28 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…7a), J NH $ showed a linear correlation to c NH $ . A linear dependency between J NH $ and c NH $ has already been observed in laboratory experiments with Phaseolus vulgaris (Farquhar et al, 1980 ;Van Hove et al, 1987) ; Populus euamericana (Van Hove et al, 1989) ; Picea abies (Kesselmeier et al, 1993) ; and Hordeum vulgare . In the NO # -fumigation assays, both in the field (Fig.…”

Section: mentioning

confidence: 60%

See 1 more Smart Citation

NH₃ and NO₂ fluxes between beech trees and the atmosphere – correlation with climatic and physiological parameters

2000

View full text Add to dashboard Cite

The dynamic-chamber technique was used to investigate the correlation between NH $ and NO # fluxes and different climatic and physiological parameters : air temperature ; relative air humidity ; photosynthetic photon fluence rate ; NH $ and NO # concentrations ; transpiration rate ; leaf conductance for water vapour ; and photosynthetic activity. The experiments were performed with twigs from the sun crown of mature beech trees (Fagus sylvatica) at a field site (Ho$ glwald, Germany), and with 12-wk-old beech seedlings under controlled conditions. Both sets of experiments showed that NO # and NH $ fluxes depended linearly on NO # and NH $ concentration, respectively, in the concentration ranges representative for the field site studied, and on watervapour conductance as a measure for stomatal aperture. The NO # compensation point determined in the field studies (the atmospheric NO # concentration with no net NO # flux) was 1n8-1n9 nmol mol −" . The NH $ compensation point varied between 3n3 and 3n5 nmol mol −" in the field experiments, and was 3n0 nmol mol −" in the experiments under controlled conditions. The climatic factors T and PPFR were found to influence both NO # and NH $ fluxes indirectly, by changing stomatal conductance. Whilst NO # flux showed a response to changing relative humidity that could be explained by altered stomatal conductance, increased NH $ flux with increasing relative humidity ( 50 %) depended on other factors. The exchange of NO # between above-ground parts of beech trees and the atmosphere could be explained exclusively by uptake or emission of NO # through the stomata, as indicated by the quotient between measured and predicted NO # conductance of approx. 1 under all environmental conditions examined. Neither internal mesophyll resistances nor additional sinks could be observed for adult trees or for beech seedlings. By contrast, the patterns of NH $ flux could not be explained by an exclusive exchange of NH $ through the stomata. Deposition into additional sinks on the leaf surface, as indicated by an increase in the quotient between measured and predicted NH $ conductance, gained importance in high air humidity, when the stomata were closed or nearly closed and\or when atmospheric NH $ concentrations were high. Although patterns of NH $ gas exchange did not differ between different months or years at high NH $ concentrations (c. 140 nmol mol −" ), it must be assumed that emission or deposition fluxes at low ambient NH $ concentration (0n8 and 4n5 nmol mol −" ) might vary significantly with time because of variation in the NH $ compensation point.

show abstract

Section: mentioning

confidence: 60%

“…Van Hove et al (1987) observed a linear correlation between stomatal conductance of P. vulgaris and J NH $ , and reported that deposition of NH $ on the cuticles was negligible. Our results suggest that it plays a significant role under particular environmental conditions.…”

mentioning

confidence: 99%

NH₃ and NO₂ fluxes between beech trees and the atmosphere – correlation with climatic and physiological parameters

2000

View full text Add to dashboard Cite

show abstract

“…NH 3 is highly reactive and forms soluble products inside the leaves as can be exemplified by the following equation: NH 3 + H 2 O ↔ NH 4 + + OH -. The major determinants for NH 3 deposition are stomatal conductance and environmental conditions, particularly humidity and light that increase the rate of deposition (Van Hove et al 1987).…”

Section: Resultsmentioning

confidence: 99%

Effects of different ammoniacal nitrogen sources on N-metabolism of the atmospheric bromeliad Tillandsia pohliana Mez

Tamaki

Mercier

2001

Rev. bras. Bot.

View full text Add to dashboard Cite

-(Effects of different ammoniacal nitrogen sources on N-metabolism of the atmospheric bromeliad Tillandsia pohliana Mez). Increasing levels of atmospheric ammonia from anthropogenic sources have become a serious problem for natural vegetation. Short-term effects of different ammoniacal sources on the N metabolism of Tillandsia pohliana, an atmospheric bromeliad, were investigated. One-year-old, aseptically grown plants were transferred to a modified Knudson medium lacking N for three weeks. Plants were subsequently transferred to Knudson media supplemented with 0.5, 1.0, or 1.5 mM of N in the forms of NH 3 or NH 4 + as the sole N source. The activities of glutamine synthetase (GS) and glutamate dehydrogenase (GDH-NADH) were determined after 40 h. The GS activity was stimulated significantly by increasing the levels of the gaseous form. The GDH-NADH activity increased significantly under increasing N concentrations with NH 3 , while no significant differences were observed with NH 4 + as a N source. These results may reflect a faster NH 3 absorption by T. pohliana compared to NH 4 + uptake. The increased activity of GDH-NADH in NH 3 treatment may play a role in protecting the cells from the toxic effects of increased endogenous level of free ammonium. A raise in the concentration of N, especially in the form of NH 3 , greatly increased the content of free amino acids and soluble proteins. A possible utilisation of T. pohliana to evaluate the changes of atmospheric gaseous ammonia is proposed.RESUMO -(Efeitos de diferentes fontes amoniacais sobre o metabolismo de nitrogênio da bromélia atmosférica Tillandsia pohliana Mez). As fontes antropogênicas de amônia atmosférica têm se tornado um problema sério para a vegetação natural. No presente trabalho foram analisados os efeitos da aplicação de diferentes fontes amoniacais sobre o metabolismo do nitrogênio de Tillandsia pohliana, uma bromélia atmosférica. As sementes foram germinadas in vitro e as plantas cresceram em meio de cultura de Knudson por um ano. Após esse período, elas foram transferidas para o meio de Knudson sem as fontes nitrogenadas originais. Três semanas depois, as plantas foram colocadas nesse mesmo meio de cultura, porém, suplementado com 0,5, 1,0 ou 1,5 mM de N nas formas de NH 3 ou NH 4 + como fontes únicas de nitrogênio. O tempo de exposição a essas fontes foi de 40 h. As atividades enzimáticas da glutamina sintetase (GS) e da glutamato desidrogenase (GDH-NADH) foram analisadas. Os teores endógenos de amônio, de aminoácidos livres e de proteínas solúveis, presentes nos eixos caulinares dessa bromélia, também, foram determinados. As atividades da GS e da GDH-NADH foram estimuladas, significativamente, com o aumento do nitrogênio na forma gasosa. Esses resultados podem refletir a rápida absorção de NH 3 pela planta. Entretanto, a atividade aminante da GDH não apresentou diferença significativa em resposta ao aumento do N na forma de NH 4 + . Provavelmente, o incremento na atividade da GDH-NADH, no tratamento com NH 3 , está relacionado com...

show abstract

“…NH $ enters the leaf mainly through stomata (van Hove et al, 1987(van Hove et al, , 1989(van Hove et al, , 1991Husted & Schjoerring, 1995a ; and uptake is driven by the NH $ concentration gradient between the atmosphere and the substomatal cavity. A high NH $ concentration within the leaf lowers NH $ uptake or may even cause NH $ emissions when the atmospheric NH $ concentration is below the internal concentration.…”

mentioning

confidence: 99%

“…Under field conditions, where leaf surfaces may be wet or covered by hygroscopic salts, NH $ adsorption to leaf surfaces may substantially increase the NH $ flux to the plant canopy (Erisman & Wyers, 1993 ;Burkhardt & Eiden, 1994 ;Sutton et al, 1995). Controlled-chamber studies with Phaseolus vulgaris (van Hove et al, 1987(van Hove et al, , 1989, Populus euamericana (van Hove et al, 1991), H. vulgare (Husted & Schjoerring, 1995a) and Brassica napus revealed that NH $ uptake through the cuticle was indeed negligible. Dueck et al (1991) measured foliar NH $ uptake of Deschampsia flexuosa and Calluna vulgaris by supplying "&NH $ and did not observe significant "&NH $ uptake through the leaf cuticle.…”

mentioning

confidence: 99%

Uptake and utilization of atmospheric ammonia in three native Poaceae species: leaf conductances, composition of apoplastic solution and interactions with root nitrogen supply

et al. 1999

View full text Add to dashboard Cite

The physiological potential for acquisition of atmospheric ammonia (NH $ ) was investigated in three European meadow grasses (Arrhenatherum elatius, Bromus erectus and Brachypodium pinnatum) competing in chalk grasslands. Experiments were carried out with plants cultivated for about three months on a soil-sand mixture at high root nitrogen supply or in nutrient solutions at low root nitrogen supply. Two different root nitrogen regimes were applied to the solution-grown plants : 130 µmol NO $ plant −" wk −" (approx. 50 kg nitrogen ha −" in three months) ; or 130 µmol NO $ − plus 130 µmol NH % + plant −" wk −" . Each regime was combined with two levels of NH $ fumigation (0 and 70 nmol mol −" air for 24 d). Uptake of gaseous NH $ in the shoots was investigated under controlled environmental conditions including NH $ concentrations ranging from 0 to 30 nmol mol −" air. Concurrently, photosynthesis, glutamine synthetase activity, nitrogen allocation, biomass allocation and apoplastic cation composition were measured. For A. elatius, the influence of photorespiration on NH $ acquisition was also assessed.Independently of plant nitrogen status, ammonia compensation points in A. elatius and B. erectus plants were 0.5 nmol mol −" . The total leaf conductance to NH $ absorption remained constant at increasing NH $ concentrations, showing that the capacity for assimilation was unaltered. Whereas internal factors in the leaves did not cause differences in the potential for NH $ acquisition between the species, other factors of NH $ acquisition were quite different : B. erectus had higher stomatal conductance and, thus, higher NH $ uptake rates per unit leaf area compared to A. elatius and B. pinnatum ; higher stomatal conductances of B. erectus were to a large extent offset by a lower leaf area per plant, resulting from a lower growth rate and thicker leaves than in the two other species. The rate of photorespiration in Arrhenatherum constituted at least 15% of the net photosynthetic rate. Surprisingly, suppression of photorespiration indicated that NH $ uptake was supported by photorespiration. Bromus responded to fumigation with 70 nmol NH $ mol −" air for 24 d by lowering the root : shoot ratio and increasing the nitrogen concentration in the stem dry matter. The total leaf conductance to NH $ uptake decreased in all three species upon exposure to NH $ , while the stomatal conductance was unaffected. The NH $ exposure caused lower apoplastic concentrations of H + , Mg# + and Ca# + in A. elatius and B. erectus.

show abstract

Analysis of the uptake of atmospheric ammonia by leaves of Phaseolus vulgaris L.

Cited by 67 publications

References 16 publications

NH₃ and NO₂ fluxes between beech trees and the atmosphere – correlation with climatic and physiological parameters

NH₃ and NO₂ fluxes between beech trees and the atmosphere – correlation with climatic and physiological parameters

Effects of different ammoniacal nitrogen sources on N-metabolism of the atmospheric bromeliad Tillandsia pohliana Mez

Uptake and utilization of atmospheric ammonia in three native Poaceae species: leaf conductances, composition of apoplastic solution and interactions with root nitrogen supply

Contact Info

Product

Resources

About

Analysis of the uptake of atmospheric ammonia by leaves of Phaseolus vulgaris L.

Cited by 67 publications

References 16 publications

NH3 and NO2 fluxes between beech trees and the atmosphere – correlation with climatic and physiological parameters

NH3 and NO2 fluxes between beech trees and the atmosphere – correlation with climatic and physiological parameters

Effects of different ammoniacal nitrogen sources on N-metabolism of the atmospheric bromeliad Tillandsia pohliana Mez

Uptake and utilization of atmospheric ammonia in three native Poaceae species: leaf conductances, composition of apoplastic solution and interactions with root nitrogen supply

Contact Info

Product

Resources

About

NH₃ and NO₂ fluxes between beech trees and the atmosphere – correlation with climatic and physiological parameters

NH₃ and NO₂ fluxes between beech trees and the atmosphere – correlation with climatic and physiological parameters