Analysis of the Volatile Components in the Leaves of <i>Cinnamomum camphora</i> by Static Headspace Gas Chromatography Mass Spectrometry Combined with Accurate Weight Measurement

Zhu, Fangyuan; Guo, Yinlong

doi:10.1002/cjoc.201090248

Cited by 9 publications

(9 citation statements)

References 15 publications

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“…GOBPs are postulated to be involved in perception of plant volatiles. OachGOBP2 binding with 19 chemicals (mostly are volatiles of camphor tree) (Liu et al., ; Zhu and Guo, ) were tested. Eight of the 19 plant volatiles were capable of displacing 1‐NPN from the OachGOBP2/1‐NPN complex with IC 50 < 32 μM and K i ranged from 1.08 to 20.04 μM, whereas the other 11 plant volatiles were not good ligands of OachGOBP2.…”

Section: Resultsmentioning

confidence: 99%

MOLECULAR CHARACTERIZATION, EXPRESSION PATTERNS, AND LIGAND‐BINDING PROPERTIES OF TWO ODORANT‐BINDING PROTEIN GENES FROM Orthaga achatina (BUTLER) (LEPIDOPTERA: PYRALIDAE)

Liu

et al. 2012

Arch Insect Biochem Physiol

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It is postulated that insect pheromone-binding proteins (PBPs) are involved in sex pheromone reception, while the general odorant-binding proteins (GOBPs) are involved in reception of the general odorants including plant volatiles. However, this functional specificity is not completely conclusive. In the present study, full-length sequences of two new OBP genes were molecularly identified as OachPBP1 and OachGOBP2 from Orthaga achatina, an important pest of the camphor tree Cinnamomum camphora. Quantification of transcript levels by qRT-PCR showed that the two genes highly expressed in antennae, with OachPBP1 male-biased and OachGOBP2 similar between sexes. These expression patterns are consistent with the generally proposed functions of PBPs and GOBPs. With the recombinant proteins obtained by a bacterial expression system, the binding specificity of these proteins was further investigated and compared using the competitive binding assay. OachPBP1 exhibited high binding affinities with all three putative sex pheromones and 10 pheromone analogs, supporting its role in pheromone reception. On the other hand, in addition to binding with some plant volatiles, OachGOBP2 surprisingly displayed similar or even higher binding affinities with the sex pheromones than OachPBP1. Therefore, we propose that OachGOBP2 might play roles in reception of sex pheromone. Additionally, plant volatiles farnesol and farnesene showed high binding with both OachGOBP2 and OachPBP1, suggesting that these volatile chemicals have regulatory functions in the behavior of O. achatina.

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Section: Resultsmentioning

confidence: 99%

MOLECULAR CHARACTERIZATION, EXPRESSION PATTERNS, AND LIGAND‐BINDING PROPERTIES OF TWO ODORANT‐BINDING PROTEIN GENES FROM Orthaga achatina (BUTLER) (LEPIDOPTERA: PYRALIDAE)

Liu

et al. 2012

Arch Insect Biochem Physiol

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“…5 tandem mass spectrometry (MS/MS) technology is an important tool to confirm the elemental composition of compounds and distinguish isomers and isobars. [6][7][8][9] the elemental composition of compounds could be confirmed by high-resolution mass spectrometry, 1,10,11 while they could also be confirmed by exploiting the tandem mass spectra of isotopic peaks from the precursor ion. 11 the product ion scan supplied the fragmentation information of the precursor ion, 6,[12][13][14] which can be used for the discrimination of parent ions with the same molecular weight and reducing the false matching rate.…”

Section: Europeanmentioning

confidence: 99%

Analysis of the Volatile Compounds in Senecio Scandens Buch-Ham by Gas Chromatography—Tandem Mass Spectrometry Based on Diversified Scan Technologies

Guo

2011

Eur J Mass Spectrom (Chichester)

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Static headspace gas chromatography-tandem mass spectrometry was used to identify volatile compounds from Senecio scandens Buch-Ham. The elemental composition of compounds was confirmed by exploiting the tandem mass spectra of isotopic peaks from the precursor ion. Some isomers were well distinguished by the diversified scan technologies of tandem mass spectrometry (MS/MS). The MS/MS included a product ion scan, a precursor ion scan and a neutral loss scan. The results showed that 46 volatile compounds were completely identified, and the great of majority compounds were α-pinene (11.93%), n-caproaldehyde (9.02%) and dehydrosabinene (6.22%). This qualitative method is convenient and accurate and can be considered as a complementary identification method for the qualitative analysis of volatile compounds in complex samples.

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“…[15,16] Gas chromatography coupled to mass spectrometry (GC-MS) is a well established and powerful technique for the analysis of volatile compounds. [1,[17][18][19][20] Triple quadrupole mass spectrometry (QqQ MS), a tandem MS, can distinguish isomers with the similar retention index, which could not be solved by mass spectrometry. [21] Time of flight mass spectrometry (TOF MS), a highresolution MS, can provide accurate mass which improves the analyte identification.…”

Section: Introductionmentioning

confidence: 99%

Analysis of Trace‐Level Volatile Compounds in Fresh Turf Crop (Lolium perenne L.) by Gas Chromatography Quadrupole Time‐of‐Flight Mass Spectrometry

Hong

Kang

et al. 2013

Chin. J. Chem.

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In the traditional research of volatile compounds, some trace-level compounds could not be identified by gas chromatography-mass spectrometry. Target and post-targeted methods were applied in the investigation of trace-level volatile compounds in fresh turf crop (Lolium perenne L.) based on gas chromatography in combination with hybrid quadrupole time-of-flight mass spectrometry. According to literatures published, a target analysis was performed by using retention index, accurate masses of characteristic ions and second-stage mass spectra (MS 2 spectra). And a series of experiments showed that low electron impact energy was beneficial to the improvement of the abundances of low abundance molecular ion peak. Enhancing the abundances of low abundance molecular ion peak was beneficial to qualitative analysis. Totally, 60 volatile compounds were identified, the great majority compounds of which were benzeneacetaldehyde (14.8%), 2,5-dimethyl-pyrazine (9.6%), and hexanal (9.3%). Identification was complied by mass spectral search, retention index and accurate masses of characteristic ions.

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Analysis of the Volatile Components in the Leaves of Cinnamomum camphora by Static Headspace Gas Chromatography Mass Spectrometry Combined with Accurate Weight Measurement

Cited by 9 publications

References 15 publications

MOLECULAR CHARACTERIZATION, EXPRESSION PATTERNS, AND LIGAND‐BINDING PROPERTIES OF TWO ODORANT‐BINDING PROTEIN GENES FROM Orthaga achatina (BUTLER) (LEPIDOPTERA: PYRALIDAE)

MOLECULAR CHARACTERIZATION, EXPRESSION PATTERNS, AND LIGAND‐BINDING PROPERTIES OF TWO ODORANT‐BINDING PROTEIN GENES FROM Orthaga achatina (BUTLER) (LEPIDOPTERA: PYRALIDAE)

Analysis of the Volatile Compounds in Senecio Scandens Buch-Ham by Gas Chromatography—Tandem Mass Spectrometry Based on Diversified Scan Technologies

Analysis of Trace‐Level Volatile Compounds in Fresh Turf Crop (Lolium perenne L.) by Gas Chromatography Quadrupole Time‐of‐Flight Mass Spectrometry

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