Analysis of the vomeronasal receptor repertoire, expression and allelic diversity in swine

Dinka, Hunduma; Lee, Wang Jae; Ha, Heekyun; Cho, Hyesun; Choi, Mi Hyun; Choi, Hojun; Kim, Jin‐Hoi; Soundarajan, Nagasundarapandian; Park, Jin-Ki; Park, Chankyu

doi:10.1016/j.ygeno.2015.10.003

Cited by 14 publications

(15 citation statements)

References 42 publications

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“…; Dinka et al. ), whereas that of scaled reptiles exclusively possesses V2Rs‐G αo and degenerates functional V1Rs (Kondoh et al. ; Brykczynska et al.…”

Section: Discussionmentioning

confidence: 99%

“…The VNO of rodents, marsupials and monotremes possesses both V1Rs-G ai2 and V2Rs-G ao (Jia & Halpern, 1996;Shi & Zhang, 2007;Brykczynska et al 2013). On the other hand, the vomeronasal system exclusively expresses V1Rs-G ai2 and degenerates functional V2Rs in most carnivores, ungulates and platyrrhini primates (Takigami et al 2000(Takigami et al , 2004Shi & Zhang, 2007;Young & Trask, 2007;Young et al 2010;Salazar & S anchez-Quinteiro, 2011;Hohenbrink et al 2012;Salazar et al 2013;Brykczynska et al 2013;Dinka et al 2016), whereas that of scaled reptiles exclusively possesses V2Rs-G ao and degenerates functional V1Rs (Kondoh et al 2013;Brykczynska et al 2013). The present immunohistochemical findings showed that G ai2 , but not G ao , is expressed in dendritic knobs of the vomeronasal sensory cells in the bear where receptors were located, indicating that the vomeronasal system mainly expresses V1R-G ai2 in bears as it does in other carnivores.…”

Section: Discussionmentioning

confidence: 99%

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Morphological and histological features of the vomeronasal organ in the brown bear

Tomiyasu

Kondoh

Sakamoto³

et al. 2017

Journal of Anatomy

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The vomeronasal organ (VNO) is a peripheral receptor structure that is involved in reproductive behavior and is part of the vomeronasal system. Male bears exhibit flehmen behavior that is regarded as the uptake of pheromones into the VNO to detect estrus in females. However, the morphological and histological features of the VNO in bears have not been comprehensively studied. The present study investigated the properties and degree of development of the VNO of the brown bear by histological, histochemical and ultrastructural methods. The VNO of bears was located at the same position as that of many other mammals, and it opened to the mouth like the VNO of most carnivores. The shape of the vomeronasal cartilages and the histological features of the sensory epithelium in the bear VNO were essentially similar to those of dogs. Receptor cells in the VNO of the bear possessed both cilia and microvilli like those of dogs. The dendritic knobs of receptor cells were positive for anti-G protein alpha-i2 subunit (G ) but negative for anti-G protein alpha-o subunit indicating preferential use of the V1R-G pathway in the vomeronasal system of bears, as in other carnivores. The VNO of the bear possessed three types of secretory cells (secretory cells of the vomeronasal gland, multicellular intraepithelial gland cells and goblet cells), and the present findings showed that the secretory granules in these cells also had various properties. The vomeronasal lumen at the middle region of the VNO invaginated toward the ventral region, and this invagination contained tightly packed multicellular intraepithelial gland cells. To our knowledge, this invagination and intraepithelial gland masses in the VNO are unique features of brown bears. The VNO in the brown bear, especially the secretory system, is morphologically well-developed, suggesting that this organ is significant for information transmission in this species.

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“…; Dinka et al. ), whereas that of scaled reptiles exclusively possesses V2Rs‐G αo and degenerates functional V1Rs (Kondoh et al. ; Brykczynska et al.…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Morphological and histological features of the vomeronasal organ in the brown bear

Tomiyasu

Kondoh

Sakamoto³

et al. 2017

Journal of Anatomy

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“…PCR products were analyzed on a 1% agarose gel, and the genotype was determined according to the product sizes (817 and 676 bp for insertion and wildtype alleles, respectively). PCR products were prepared for direct sequencing as described previously [ 21 ]. Sequencing reactions were carried out with 30 ng of amplicons from homozygous individuals and 2 pmol of primers identical to PCR amplification for both directions using an ABI PRISM BigDye TM Terminator Cycle Sequencing Kit (Applied Biosystems, Massachusetts, USA) following the manufacturer’s protocol.…”

Section: Methodsmentioning

confidence: 99%

Tracing the Origin of the RSPO2 Long-Hair Allele and Epistatic Interaction between FGF5 and RSPO2 in Sapsaree Dog

Kang

Ahn

Youk

et al. 2022

Genes

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Genetic analysis of the hair-length of Sapsaree dogs, a Korean native dog breed, showed a dominant mode of inheritance for long hair. Genome-Wide Association Study (GWAS) analysis and subsequent Mendelian segregation analysis revealed an association between OXR1, RSPO2, and PKHD1L1 on chromosome 13 (CFA13). We identified the previously reported 167 bp insertion in RSPO2 3’ untranslated region as a causative mutation for hair length variations. The analysis of 118 dog breeds and wolves revealed the selection signature on CFA13 in long-haired breeds. Haplotype analysis showed the association of only a few specific haplotypes to the breeds carrying the 167 bp insertion. The genetic diversity in the neighboring region linked to the insertion was higher in Sapsarees than in other Asian and European dog breeds carrying the same variation, suggesting an older history of its insertion in the Sapsaree genome than in that of the other breeds analyzed in this study. Our results show that the RSPO2 3’ UTR insertion is responsible for not only the furnishing phenotype but also determining the hair length of the entire body depending on the genetic background, suggesting an epistatic interaction between FGF5 and RSPO2 influencing the hair-length phenotype in dogs.

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“…The latter figure is rather surprising because we identified up to 55 new V2R, a family completely degenerated in most mammals. Additionally, following the same criteria as Dinka et al 2016, we classified the total VR genes repertoire into 177 intact VR (encoding > 300 amino acids (aas)) and 22 partial VR (> 100 aas and < 300 aas).…”

Section: Resultsmentioning

confidence: 99%

“…However, we can argue that in mice VR expression was found in testis developing germ cells, whereas in rabbits no expression was noticed from the embryonic day 12 to the postnatal day 80, thus being concentrated in mature animals (186-548 days). In swine, V1R genes were highly expressed in the VNO and in testis, whereas their expression in other tissues was very low (Dinka et al 2016). The function of V1Rs in testis remains still unknown, but other chemical receptors such as olfactory receptors (ORs) were also expressed in this gonad in mice, rats and humans (Spehr et al 2003; Makeyeva et al 2020), thus suggesting another functional roles other than stimulating sensory systems.…”

Section: Discussionmentioning

confidence: 99%

A comprehensive analysis of vomeronasal organ transcriptome reveals variable gene expression depending on age and function in rabbits

Villamayor¹,

Robledo

Fernandez³

et al. 2020

Preprint

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The vomeronasal organ (VNO) is a chemosensory organ specialized in the detection of pheromones and consequently the regulation of behavioural responses mostly related to reproduction. VNO shows a broad variation on its organization, functionality and gene expression in vertebrates, and although the species analyzed to date have shown very specific features, its expression patterns have only been well-characterized in mice. Despite rabbits represent a model of chemocommunication, unfortunately no genomic studies have been performed on VNO of this species to date. The capacity of VNO to detect a great variety of different stimuli suggests a large number of genes with complex organization to support this function. Here we provide the first comprehensive gene expression analysis of the rabbit VNO through RNA-seq across different sexual maturation stages. We characterized the VNO transcriptome, updating the number of the two main vomeronasal receptor (VR) families, 129 V1R and 70 V2R. Among others, the expression of transient receptor potential channel 2 (TRPC2), a crucial cation channel generating electrical responses to sensory stimulation in vomeronasal neurons, along with the specific expression of some fomyl-peptide receptors and H2-Mv genes, both known to have specific roles in the VNO, revealed a the particular gene expression repertoire of this organ, but also its singularity in rabbits. Moreover, juvenile and adult VNO transcriptome showed consistent differences, which may indicate that these receptors are tuned to fulfill specific functions depending on maturation age. We also identified VNO-specific genes, including most VR and TRPC2, thus confirming their functional association with the VNO. Overall, these results represent the genomic baseline for future investigations which seek to understand the genetic basis of behavioural responses canalized through the VNO.HIGHLIGHTSFirst description of the rabbit vomeronasal organ (VNO) transcriptomeVNO contains a unique gene repertoire depending on the speciesHigh fluctuation of the VNO gene expression reveals changes dependent on age and specific functionsMost vomeronasal-receptors (VR) and transient receptor potential channel 2 (TRPC2) genes are VNO-specificReproduction-related genes shows a wide expression pattern

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Analysis of the vomeronasal receptor repertoire, expression and allelic diversity in swine

Cited by 14 publications

References 42 publications

Morphological and histological features of the vomeronasal organ in the brown bear

Morphological and histological features of the vomeronasal organ in the brown bear

Tracing the Origin of the RSPO2 Long-Hair Allele and Epistatic Interaction between FGF5 and RSPO2 in Sapsaree Dog

A comprehensive analysis of vomeronasal organ transcriptome reveals variable gene expression depending on age and function in rabbits

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