Analysis of time and temperature stability of EDTA anticoagulation whole blood for complete blood count parameters with the use of Abbott CELL‐DYN Sapphire hematology analyzer

Zhang, Chi; Zhang, Hui; Guo, Li; Yang, Fei

doi:10.1111/ijlh.13111

Cited by 2 publications

(7 citation statements)

References 12 publications

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“…For differential leukocyte counts, a rapid increase of the number of invalid measurements over time was observed, especially beyond 72 h of storage at 4 and 30°C (Supplementary Table S3). Previous studies on differential leukocyte counts have reported acceptable stability between 48 and 72 h at 4°C or room temperature 10,14,21–23 . Differences between these reports and our results may be attributable to the number of samples with differential leukocyte counts outside the reference range in our study, which remained less stable over time compared to differential counts within the reference range (Supplementary Figure S5).…”

Section: Discussioncontrasting

confidence: 93%

“…Differences between these reports and our results may be attributable to the number of samples with differential leukocyte counts outside the reference range in our study, which remained less stable over time compared to differential counts within the reference range (Supplementary Figure S5). Furthermore, selected stability criteria and instruments can also partly explain differences in conclusions between our and other studies 10,14 . In addition, stability of leukocyte and differential counts may have been affected by using the primary sample tube for all measurements over time, thus temporarily reheating samples stored at 4°C at each time point.…”

Section: Discussionmentioning

confidence: 84%

“…Mean relative difference for leukocyte counts was within desirable TEa up to 48 or 72 h when stored at 4°C or room temperature, respectively. Previous studies have concluded similar stability, albeit with different stability criteria 10,14 …”

Section: Discussionmentioning

confidence: 89%

“…Previous studies have concluded similar stability, albeit with different stability criteria. 10,14 For differential leukocyte counts, a rapid increase of the number of invalid measurements over time was observed, especially beyond 72 h of storage at 4 and 30 C (Supplementary Table S3). Previous studies on differential leukocyte counts have reported acceptable stability between 48 and 72 h at 4 C or room temperature.…”

Section: Stabilitymentioning

confidence: 99%

“…Previous research regarding venous and capillary blood sampling to measure CBC parameters has primarily focused on comparability of results and, to a lesser extent, on short‐term stability (up to 24–72 h) 8–16 . Data on comparability, longer term stability, and influence of sample storage conditions for current generations of hematology analyzers is limited.…”

Section: Introductionmentioning

confidence: 99%

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Stability and comparison of complete blood count parameters between capillary and venous blood samples

Doeleman

Esseveld

Huisman

et al. 2023

Int J Lab Hematology

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IntroductionThis study assessed the comparability of complete blood count (CBC) parameters between capillary and venous samples, and extended previous research by examining the influence of different storage temperatures on CBC stability up to 7 days after sample collection.MethodsVenous and capillary blood samples were collected from 93 adult patients. Hemoglobin (Hb), hematocrit (Ht), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), mean platelet volume (MPV), leukocytes, lymphocytes, basophils, eosinophils, erythrocytes, red cell distribution width (RDW), immature granulocytes (IG), immature reticulocyte fraction (IRF), monocytes, neutrophils, platelets, and reticulocytes were measured. Deming regression and mean relative differences between venous and capillary measurements were contrasted with desirable total allowable error (TEa). Stability was assessed in 20–27 venous blood samples stored at 4, 21–22, or 30°C, and analyzed at 0, 24, 48, 72, 96, 120, 144, and 168 h. Mean relative change with respect to baseline measurements was compared to the desirable TEa to determine acceptable stability.ResultsDeming regression demonstrated strong linear correlations and acceptable variation between venous and capillary measurements. Erythrocytes, Hb, Ht, MCH, MCV, RDW, reticulocytes, and platelets showed acceptable stability for at least 96 h at 4°C. Mean relative change exceeded desirable TEa after 24 h at 30°C for all parameters, except erythrocytes, Hb, leukocytes, and MCH.ConclusionClinical laboratory specialists and clinicians should be aware of potential differences between venous and capillary measurements, and the influence of storage conditions. Clinical validity of delayed CBC analysis depends on the clinical situation and required precision of the result.

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Section: Discussioncontrasting

confidence: 93%

Section: Discussionmentioning

confidence: 84%

Section: Discussionmentioning

confidence: 89%

Section: Stabilitymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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