Analysis of time-course gene expression profiles of a periodontal ligament tissue model under compression

Liu, Yu; Li, Meile; Tan, Lijun; Huang, Shengbin; Zhao, Lixing; Tang, Tian; Li, Jun; Zhao, Zhihe

doi:10.1016/j.archoralbio.2012.10.006

Cited by 44 publications

(43 citation statements)

References 37 publications

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“…As many other studies and our previous microarray data show, osteoclastogenesis inducer gene expression increased and osteoclastogenesis inhibitors dropped down under static mechanical compression. 5,6 In the present study, Cox2 and RANKL mRNA also increased significantly after hours of mechanical stress. However, in the present study OPG mRNA was not changed in loaded hPDLCs.…”

Section: Discussionsupporting

confidence: 65%

“…[1][2][3][4] Recently, we found that the expression of potential osteoclastogenesis inducers was upregulated in a compressed PDLCs model, including receptor activator of nuclear factor kB Ligand (RANKL), cyclooxygenase-2 (Cox2), while the expression of osteoprotegerin (OPG) was downregulated. 5,6 Furthermore, prostaglandin E2 (PGE2) may increase RANKL mRNA expression in compressed PDLCs. 3 However, the mechanism by which PDLCs sense and transduce mechanical signals is still unclear.…”

Section: Introductionmentioning

confidence: 99%

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Functional role of mechanosensitive ion channel Piezo1 in human periodontal ligament cells

Jin

Wang

et al. 2015

The Angle Orthodontist

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Objective: To evaluate the function of Piezo1, an evolutionarily conserved mechanically activated channel, in periodontal ligament (PDL) tissue homeostasis under compressive loading. Materials and Methods: Primary human PDL cells (hPDLCs) were isolated, cultured, and then subjected to 2.0 g/cm 2 static compressive loading for 0.5, 3, 6, and 12 hours, respectively. The expressions of Piezo1 and osteoclastogenesis marker gene were assessed by semiquantitative reverse transcription-polymerase chain reaction. In addition, Piezo1 inhibitor, GsMTx4, was used to block the function of Piezo1, and tumor necrosis factor-a was also used as a positive control. After 12 hours of compressive loading the PDLCs were co-cultured with murine monocytic cell line RAW264.7. Immunofluorescence, western blot, enzyme-linked immunosorbent assay, and tartrate-resistant acid phosphatase staining were also used to test the potency of PDLCs to induce osteoclastogenesis and the activation of nuclear factor (NF)-kB. Results: Piezo1, cyclooxygenase-2, receptor activator of NF-kB ligand, and prostaglandin E2 were significantly upregulated under static compressive stimuli. GsMTx4 repressed osteoclastogenesis in the mechanical stress-pretreated PDLCs-RAW264.7 co-culture system. Furthermore, NF-kB signaling pathway was involved in the mechanical stress-induced osteoclastogenesis. Conclusions: Piezo1 exerts a transduction role in mechanical stress-induced osteoclastogenesis in hPDLCs. (Angle Orthod. 2015;85:87-94.)

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Section: Discussionsupporting

confidence: 65%

Section: Introductionmentioning

confidence: 99%

Functional role of mechanosensitive ion channel Piezo1 in human periodontal ligament cells

Jin

Wang

et al. 2015

The Angle Orthodontist

Self Cite

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“…Moreover, our group had previously reported that though static compression rather than cyclic force was applied to the PDLCs, cytoskeleton-related genes showed significant changes even 72 hours after force application. 13 This observation proves the continuous effects from static compression on the PDLCs. Therefore, it would be more reasonable to involve both hypoxia and compression when exploring the bone remodeling process on the pressure side regulated by the PDLCs in OTM rather than investigate a single factor.…”

Section: Discussionmentioning

confidence: 53%

“…Cells of fourth-sixth passage were used for the experiment. 13 The PDLtm was established by dripping the suspension of PDLCs (1 3 10 5 cells in approximately 2 mL medium) 5 onto the scaffolds, which were placed on a six-well plate. To exclude the confounding effects from the 2-D cultured cells attached to the bottom of the well, the PDLtms were transferred to another sixwell plate 24 hours later.…”

Section: D Culture Of Pdlcsmentioning

confidence: 99%

“…To simulate the compressed PDL under orthodontic force, a modified weight method was used as previously described. 5,13 Briefly, a cover glass was placed over each PDLtm, then a glass bottle with lead granules having a total weight of 100 g was placed on the cover glass to produce a mechanical force of 25 g/cm 2 . To induce hypoxia, the PDLtm was cultured in an incubator with an atmosphere of 2% O 2 .…”

Section: Treatment Of Hypoxia and Static Compressionmentioning

confidence: 99%

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Compression and hypoxia play independent roles while having combinative effects in the osteoclastogenesis induced by periodontal ligament cells

Yang

et al. 2015

The Angle Orthodontist

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Objective: To investigate the isolated and combined effects of compression and hypoxia on the osteoclastogenesis induced by periodontal ligament cells (PDLCs). Materials and Methods: A periodontal ligament tissue model (PDLtm) was established by 3-D culturing human PDLCs on a thin sheet of poly lactic-co-glycolic acid scaffold. The PDLtm was treated with hypoxia and/or compression for 6, 24, or 72 hours. After that, a real-time polymerase chain reaction was used for gene expression analysis. The conditioned media were used for the coculture of osteoblast and osteoclast (OC) precursors; tartrate-resistant acid phosphatase staining was done to examine OC formation. Results: Either compression or hypoxia alone significantly up-regulated the gene expression of pro-osteoclastogenic cytokines in the PDLtm and enhanced osteoclastogenesis in the cocultures, and the combination of the two had significantly stronger effects than either stimulation alone. In addition, comparing the two stimulants, we found that the osteoclastogenic property of the PDLCs peaked earlier (at 6 hours) in the compression group than in the hypoxia group (at 24 hours). Conclusions: Both compressive force and hypoxia may take part in initiating osteoclastogenesis in orthodontic tooth movement and may have combinatory effects, which could update our concepts of the mechanisms involved in the initiation of bone resorption on the pressure side of the tooth in question. (Angle Orthod. 2016;86:66-73.)

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Planning and Executing Tooth‐movement Research

Krishnan¹,

Davidovitch²,

Ramachandran³

2021

Biological Mechanisms of Tooth Movement

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Analysis of time-course gene expression profiles of a periodontal ligament tissue model under compression

Cited by 44 publications

References 37 publications

Functional role of mechanosensitive ion channel Piezo1 in human periodontal ligament cells

Functional role of mechanosensitive ion channel Piezo1 in human periodontal ligament cells

Compression and hypoxia play independent roles while having combinative effects in the osteoclastogenesis induced by periodontal ligament cells

Planning and Executing Tooth‐movement Research

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