Analysis of toxic anthraquinones and related compounds with a fused silica capillary column

Lewis, David C.; Shibamoto, Takayuki

doi:10.1002/jhrc.1240080602

Cited by 7 publications

(1 citation statement)

References 4 publications

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“…Chromatographic methods that have been used successfully to identify and quantitate anthraquinones in Cassia extracts include column chromatography on Sephadex LH-20 (Koshioka et al, 1978), capillary GC-MS (Van Eijk and Roeijmans, 1984), fused silica capillary GC (Lewis and Shibamoto, 1985), and HPLC (Oshima et al, 1986; Van Den Berg and Labadie, 1985). These approaches are relatively time-consuming; the GC methods involved derivatizing, so they were not appropriate for screening a large number of samples quickly.…”

Section: Introductionmentioning

confidence: 99%

Composition of sicklepod (Cassia obtusifolia) toxic weed seeds

Crawford¹,

McDonald²,

Friedman³

1990

J. Agric. Food Chem.

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Bulk commercial grain, such as soybeans and wheat, may be contaminated with nongrain impurities, including sicklepod seeds (Cassia obtusifolia), that coexist with the crop to be harvested. The present study was undertaken to determine the content of the major anthraquinone constituents of sicklepod seeds, as well as carbohydrate, fat, mineral, and amino acid (protein) content. Thin-layer chromatography, ultraviolet spectroscopy, and combined gas chromatography-mass spectrometry revealed the presence of known and unknown anthraquinone derivatives. Known compounds include chrysophanic acid, physcion, obtusofolin, emodin, questin, obtusin, chrysoobtusin, aurantioobtusin, and others. Rapid thinlayer chromatographic procedures were developed to screen sicklepod seeds for anthraquinone derivatives.These procedures also revealed the presence of /J-sitosterol and of flavonoids, UV-quenching compounds, and fluorescent blue compounds of unknown structure. The described methodology should facilitate screening large numbers of samples both in the laboratory and under practical field conditions. The data provide a rational basis for relating seed composition to reported muscle-damaging and other toxicological effects of sicklepod, for assessing the significance of low levels of the seeds in diets of foodproducing animals and humans, and for setting grain standards.

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Section: Introductionmentioning

confidence: 99%