Analysis of Transcription Initiation in the Panolis flammea Nuclear Polyhedrosis Virus Polyhedrin Gene

Abstract

SUMMARYThe nucleotide sequence of the polyhedrin gene of Pano/is flammea multiple nucleocapsid polyhedrosis virus (PfMNPV) has been determined. The coding sequences of this gene shared 82% similarity at the DNA level and 88% similarity at the protein level with the polyhedrin gene from Autographa califomica (Ac) MNPV. A single nucleotide deviation from the consensus transcription initiation sequence for baculovirus very late genes was identified in the PfMNPV polyhedrin gene. RNA was prepared from Mamestra brassicae larvae infected with PfMNPV and compared with RNA harvested at 24 h post-infection from AcMNPV-infected Spodopterafrugiperda

Characterization of two variants of Panolis flammea multiple nucleocapsid nuclear polyhedrosis virus

“…This region of sequence contains the 12 bp consensus sequence found upstream of all sequenced hyper-expressed very late NPV genes; however, as in the polyhedrin genes of PfMNPV(A) and MbMNPV, it is not a perfect match with the consensus proposed by Rohrmann (1986). The initiation sites of polyhedrin mRNA of both PfMNPV(A) (Oakey et al, 1989) and AcMNPV (Howard et al, 1986) lie within this sequence. The size of the polyhedrin mRNA for PfMNPV(B) was determined by Northern blot hybridization to be the same size as the 1.0 kb mRNA reported previously for PfMNPV(A) (Oakey et al, 1989) and significantly smaller than that of AcMNPV, 1.15 to 1-2 kb (data not shown).…”

“…The initiation sites of polyhedrin mRNA of both PfMNPV(A) (Oakey et al, 1989) and AcMNPV (Howard et al, 1986) lie within this sequence. The size of the polyhedrin mRNA for PfMNPV(B) was determined by Northern blot hybridization to be the same size as the 1.0 kb mRNA reported previously for PfMNPV(A) (Oakey et al, 1989) and significantly smaller than that of AcMNPV, 1.15 to 1-2 kb (data not shown). When stocks of PfMNPV(A) and PfMNPV(B) were propagated in M. brassicae it was observed that PfMNPV(A) consistently produced higher yields of polyhedra.…”

“…The resulting plasmid, pPf(B)HF 1, was used as a source of DNA for sequencing the PfMNPV(B) polyhedrin gene. The nucleotide sequence of this region was determined and comparisons with the PfMNPV(A) polyhedrin gene sequence (Oakey et al, 1989) showed the two genes to differ at only five nucleotide positions [213, T~A; 453, C~T; 585, C--,T; 606, G~A; 681, G~T; numbering from the A (position 1) of the ATG start codon of PfMNPV(A)]. These nucleotide changes did not affect the predicted amino acid sequence, which was identical in both PfMNPV(A) and PfMNPV(B).…”

Baculoviruses contain a gene for the large subunit of ribonucleotide reductase.

“…Databank searches revealed the occurrence of RR1-like sequences upstream of the polyhedrin gene in Mamestra brassicae multicapsid NPV (MbMNPV ; , Panolis flammea multicapsid NPV (PfMNPV ; Oakey et al, 1989) and Orgyia pseudotsugata single nucleocapsid NPV (OpSNPV ; Leisy et al, 1986). The predicted amino acid sequences in these viruses showed homology to the N terminus of RR1 (Fig.…”

Comparative analysis of the genomes of Rachiplusia ou and Autographa californica multiple nucleopolyhedroviruses

“…AAD00095); ManeNPV, Malacosoma neustria NPV (accession no. AAB31529); MbMNPV, Mamestra brassicae MNPV ; OpMNPV, Orgyia pseudotsugata MNPV (Ahrens et al, 1997); OpSNPV, Orgyia pseudotsugata SNPV (Leisy et al, 1986); PaflMNPV, Panolis flammea MNPV (Oakey et al, 1989); PenuNPV, Perina nuda NPV (Chou et al, 1996); RoMNPV, Rachiplusia ou MNPV (Harrison & Bonning, 1999); SeMNPV, Spodoptera exigua MNPV (IJkel et al, 1999); SfMNPV, Spodoptera frugiperda MNPV (Gonzalez et al, 1989); SpltMNPV, Spodoptera litura MNPV (Pang et al, 2001); ThorMNPV, Thysanoplusia orichalcea MNPV (accession no. AF169480); ThorSNPV, Thysanoplusia orichalcea SNPV (Cheng et al, 1998); TnSNPV, Trichoplusia ni SNPV (Fielding & Davison, 1999); WisiNPV, Wiseana signata NPV (accession no.…”