Analysis of volatile fatty acids by capillary gas chromatography using on‐column injection of aqueous solutions

Organic solvent effects in reversed-phase liquid chromatography in relation to column testing

“…This confirms previously published results [32], whichever solvent in use. This confirms previously published results [32], whichever solvent in use.…”

“…In order to obtain a reliable test, it had been established that mobile phases had to be buffered [31,37]. Buffer was prepared by dissolving 20mM of sodium acetate in pure water and adjusting the pH to 5.00 at 25~ with concentrated acetic acid.…”

Possibilities and pitfalls in defining selectivity in HPLC

“…paration (modifier selectivity) [35,36]. In addition, for ionized or ionizable analytes changes in selectivity can be achieved by changing the pH and/or the concentration of ion-pair-reagent.…”

Cheap C18‐modified Silica Monolith Particles as HPLC Stationary Phase of Good Separation Efficiency

“…[1][2][3][4][5] The columns packed with particles have a high efficiency but they are accompanied with a high column back pressure due to lower permeability, while the monolithic columns have a high permeability but they result in inferior separation efficiency for the analysis of small molecules in HPLC. [6][7][8][9][10][11][12][13] In our laboratory, we have been using the pseudo-monolithic silica particles with C-18 ligand 1,14,15 or polystyrene film 1,2,[16][17][18] as packing materials. In the current study, porous partially sub-2 μm silica monolith particles have been prepared by a sol-gel process with a six times increased production scale compared with our previous work.…”