Analysis of Xanthophylls in Corn by HPLC

Moros, E E; Darnoko, D.; Cheryan, Munir; Perkins, E. G.; Jerrell, John P.

doi:10.1021/jf020109l

Cited by 152 publications

(129 citation statements)

References 11 publications

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“…Commercial zein comes in two grades yellow and white zein (10). Yellow zein contains a high concentration of xanthophyll pigments (8-9%) including lutein, zeaxanthin and β-cryptoxanthin (11,12). Some of the undesirable properties of yellow zein have been attributed to xanthophyll (10).…”

Section: Introductionmentioning

confidence: 99%

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Influence of Formulation Factors on the Preparation of Zein Nanoparticles

2012

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Abstract. The main objective of the present study was to investigate the influence of various formulation parameters on the preparation of zein nanoparticles. 6,7-dihydroxycoumarin (DHC) was used as a model hydrophobic compound. The influence of pH of the aqueous phase, buffer type, ionic strength, surfactant, and zein concentration on particle size, polydispersity index, and zeta potential of DHC-loaded zein nanoparticles were studied. Smaller nanoparticles were formed when the pH was close to the isoelectric point of zein. DHC-loaded zein nanoparticles prepared using citrate buffer (pH 7.4) was better than phosphate buffer in preventing particle aggregation during lyophilization. The ionic strength did not have a significant influence on the particle size of DHC-loaded zein nanoparticles. A combination of Pluronic F68 and lecithin in 2:1 ratio stabilized the zein nanoparticles. An increase in zein concentration led to increase in particle size of DHC-loaded zein nanoparticles. The use of optimal conditions produced DHCloaded nanoparticles of 256±30 nm and an encapsulation efficiency of 78±7%. Overall, the study demonstrated the optimal conditions to prepare zein nanoparticles for drug encapsulation.

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Section: Introductionmentioning

confidence: 99%

“…Some of the undesirable properties of yellow zein have been attributed to xanthophyll (10). The hydrophobic xanthophylls are strongly bound to zein and various extraction methods have been used to decolorize zein (10,11). The purity of yellow zein is around 88-90% (10,11).…”

Section: Introductionmentioning

confidence: 99%

Influence of Formulation Factors on the Preparation of Zein Nanoparticles

2012

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“…Brito et al (2005) também observaram diminuição da pigmentação da gema de ovos de poedeiras alimentadas com farelo de gérmen de milho integral. Como o grão de milho possui maior concentração de xantofila (20,09 μg/g) em relação ao gérmen de milho (1,88 μg/g) (Moros et al, 2002), a redução na pigmentação da gema pode ter ocorrido pela menor quantidade do pigmentante.…”

Section: Resultsunclassified

“…Geralmente o consumidor prefere ovos com gemas mais alaranjadas porque associa esta cor à qualidade do ovo. O valor médio de xantofila no grão de milho é de 20,09 μg/g, portanto superior ao do gérmen de milho (1,8 μg/g) (Moros et al, 2002).…”

Section: Introductionunclassified

Farelo de gérmen de milho desengordurado na dieta de poedeiras comerciais de 28 a 44 semanas de idade

Brunelli¹,

Pinheiro

Fonseca

et al. 2010

R. Bras. Zootec.

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RESUMO -Um experimento foi conduzido para avaliar os efeitos da inclusão de farelo de gérmen de milho desengordurado na dieta de galinhas de 28 a 44 semanas de idade. Foram utilizadas 240 poedeiras Hy-Line W36 distribuídas em delineamento inteiramente casualizado, com seis níveis de farelo de germen de milho desengordurado (0, 6, 12, 18, 24 e 30%),cada um com cinco repetições de oito aves. No período experimental, que durou quatro ciclos de 28 dias, foram avaliados os parâmetros de desempenho das aves (consumo de ração, produção de ovos, peso médio do ovo, massa de ovo e conversão alimentar) e de qualidade dos ovos (gravidade específica, índice de pigmentação da gema, porcentagem de gema e albúmen, porcentagem e espessura da casca e unidade haugh). Os níveis de farelo de gérmen de milho desengordurado tiveram efeito linear negativo sobre o consumo de ração e o índice de pigmentação da gema e efeito quadrático sobre a conversão alimentar. A inclusão de farelo de gérmen de milho desengordurado na dieta não influenciou as outras variáveis estudadas. O farelo de gérmen de milho desengordurado pode ser incluído em níveis de até 21,2% em rações para galinhas poedeiras.Palavras-chave: alimento alternativo, desempenho, nutrição, produção de ovos, qualidade dos ovos Defatted corn germ meal in diets for laying hens from 28 to 44 weeks of age ABSTRACT -This experiment was conducted to evaluate the effect of including defatted corn germ meal in diets for laying hens. It was assigned two hundred and forty laying hens to a randomized design, with six diets and five replicates of eight hens per diet. Experimental diets were formulated by inclusion of defatted corn germ meal at 0, 6, 12, 18, 24 and 30% level. The period experiment was carried out for four 28-day cycles and performance (feed intake, egg production, egg weight, egg mass and feed conversion) and the egg quality (egg specific gravity, yolk color, yolk and albumen percentages, shell percentages, shell thickness and Haugh unity) were the evaluated parameters. Negative linear effect was observed for feed intake and yolk pigmentation by increasingly including defatted corn germ meal in diets for laying hens. Quadratic effect of defatted corn germ meal for feed conversion was also observed. No statistical differences were observed for any other parameters. Defatted corn germ meal can be included at 21.2% in laying hens diets.Key Words: alternative feed, egg production, egg quality, nutrition, performance IntroduçãoVários subprodutos da indústria de processamento de grãos têm potencial para uso na alimentação de aves, mas, para que sejam usados corretamente, necessitam de constante avaliação quanto à sua qualidade nutricional e ao nível de inclusão.O farelo de gérmen de milho desengordurado é um subproduto da indústria de óleo de milho e tema características nutricionais para uso na alimentação de galinhas poedeiras. O processo de obtenção do gérmen envolve moagem úmida do grão de milho, seguida da secagem, prensagem (Dermachi, 1998) e perfusão por solvente.O farelo d...

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“…Maize is a common feed supplement for chickens used to produce an intense egg yolk color. Maize contains a majority of xanthophylls (oxidized carotenoids) (Moros et al, 2002) that help in the pigmentation of egg-yolks (Na et al, 2004). Moreover, the digestive stability of xanthophylls is significantly higher than that of β-carotene (Blanquet-Diot et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

Application of a new red carotenoid pigment-producing bacterium, Enterobacter sp. P41, as feed supplement for chicken

Tanskul¹,

Khoonchumnan²,

Watanasit³

et al. 2013

Afr. J. Biotechnol.

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In order to use purple non-sulfur bacteria as feed supplement in chicken industries, screening for red pigment-producing bacteria with proteinases was done using hen feces as a target. One isolate, P 41 , with the highest proteinases activity was selected for further studies. Based on the data of biochemical and 16S rDNA sequence analysis, it was identified as Enterobacter sp. P 41 . This strain produced 0.16 mg of β-carotene with red color per gram of cell dry weight. Addition of the dried cells of Enterobacter sp. P 41 to chicken feeds did significantly intensify the egg yolk color (p = 0.000). Moreover, addition of the dried cells at the amount of 0.45 mg per kg of feed significantly increased the rate of egg-laying (p = 0.029). These results suggest that the dried cells of Enterobacter sp. P 41 might be useful as feed supplement for hens or other avian.

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Analysis of Xanthophylls in Corn by HPLC

Cited by 152 publications

References 11 publications

Influence of Formulation Factors on the Preparation of Zein Nanoparticles

Influence of Formulation Factors on the Preparation of Zein Nanoparticles

Farelo de gérmen de milho desengordurado na dieta de poedeiras comerciais de 28 a 44 semanas de idade

Application of a new red carotenoid pigment-producing bacterium, Enterobacter sp. P41, as feed supplement for chicken

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