Analysis of zearalenone and α-zearalenol in animal feed using high-performance liquid chromatography

Saeger, Sarah De; Sibanda, Liberty; Peteghem, Carlos Van

doi:10.1016/s0003-2670(03)00555-5

Cited by 81 publications

(36 citation statements)

References 12 publications

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“…Hyperestrogenic syndromes can be uterine enlargement, swelling of the vulva, prolapse of the vagina or rectum, prolonged or interrupted estrus, pseudopregnancy and reduced litter size (Doll et al 2003; Kumar et al 2008). a-and b-zearalenol (a-and b-ZOL) are hydroxylated derivatives of ZEN, and a-ZOL has a three to four times bigger estrogenic effect than ZEN, while b-ZOL is less estrogenically active (El-Sharkawy et al 1991;Saeger et al 2003).…”

Section: Introductionmentioning

confidence: 99%

Degradation of zearalenone by the extracellular extracts of Acinetobacter sp. SM04 liquid cultures

Qiu

et al. 2010

Biodegradation

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A bacterium (designated SM04) which can rapidly grow on zearalenone (ZEN) as sole carbon and energy source was isolated from agricultural soil. On the basis of 16S rDNA sequencing analysis, strain SM04 was classified as a bacterium belonging to the Acinetobacter genus. In this study, the biodegradation of ZEN by the extracellular extracts of strain SM04 liquid cultures in M1 medium and Nutrient Broth medium was examined using HPLC analysis, APCI-MS analysis, and MTT (tetrazolium salt) cell proliferation assay. Results showed no ZEN and other equally estrogenic metabolites were found after 12 h when ZEN was treated with the extracellular extracts of M1 cultures, but no significant (P < 0.01) reduction of ZEN was observed over the 12-h incubation period in the extracellular extracts of Nutrient Broth cultures. Results also indicated that some proteins in the extracellular extracts of M1 cultures were essential to ZEN degradation. The proteins in the extracellular extracts of M1 cultures and Nutrient Broth cultures were analyzed with SDS-PAGE, bands showing different intensities among the two extracellular extracts were processed for protein identification by MALDI-TOF/TOF/MS, and nine proteins from M1 cultures matched the database for Acinetobacter genus with great confidence. Furthermore, the function of some proteins identified is unknown or unconfirmed because of the lack of well-annotated genomic sequence data and protein data for Acinetobacter genus on the public database, but in further studies these data of proteins identified will be useful for screening the genes related to ZEN degradation.

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Section: Introductionmentioning

confidence: 99%

Degradation of zearalenone by the extracellular extracts of Acinetobacter sp. SM04 liquid cultures

Qiu

et al. 2010

Biodegradation

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“…HPLC gives higher sensitivity than thin layer chromatography (TLC) and it requires simpler clean-up work than those required by gas chromatography (GC) (Krska, 1998;Mateo, Mateo, Hinojo, Llorens, & Jimenez, 2002;Saeger, Sibanda, & van Peteghem, 2003;Schuhmacher, Krska, Grasserbauer, Edinger, & Lew, 1998;Visconti & Pascale, 1998). HPLC with fluorescence detection was accepted as the official first determination method of ZON by the AOAC (Ware, Zhao, Kuan, & Carman, 1999).…”

Section: Introductionmentioning

confidence: 99%

“…HPLC with fluorescence detection was accepted as the official first determination method of ZON by the AOAC (Ware, Zhao, Kuan, & Carman, 1999). Immuno-affinity columns, which provide clean extracts and minimise the interference of co-extracted matrices, have been widely used for ZON analysis in sample matrices (Krska, 1998;Llorens et al, 2002;Mateo et al, 2002;Saeger et al, 2003;Schuhmacher et al, 1998;Silva & Vargas, 2001).…”

Section: Introductionmentioning

confidence: 99%

A limited survey of zearalenone in Indonesian maize-based food and feed by ELISA and high performance liquid chromatography

et al. 2005

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“…Evidence is existing on the occurrence of fungal species in corn [62]. However, this has mainly been linked to contamination of corn, and consequently animal feed, with mycotoxins [63][64][65]. To the best of our knowledge, interference of feed-borne fungi in steroid-formation has so far not been considered.…”

Section: Discussionmentioning

confidence: 99%

Endogenous boldenone-formation in cattle: Alternative invertebrate organisms to elucidate the enzymatic pathway and the potential role of edible fungi on cattle's feed

Verheyden

Noppe

Zorn

et al. 2010

The Journal of Steroid Biochemistry and Molecular Biology

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Analysis of zearalenone and α-zearalenol in animal feed using high-performance liquid chromatography

Cited by 81 publications

References 12 publications

Degradation of zearalenone by the extracellular extracts of Acinetobacter sp. SM04 liquid cultures

Degradation of zearalenone by the extracellular extracts of Acinetobacter sp. SM04 liquid cultures

A limited survey of zearalenone in Indonesian maize-based food and feed by ELISA and high performance liquid chromatography

Endogenous boldenone-formation in cattle: Alternative invertebrate organisms to elucidate the enzymatic pathway and the potential role of edible fungi on cattle's feed

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