Analytical and clinical evaluation of the Bayer ADVIA Centaur® homocysteine assay

Tewari, Prakash C.; Zhang, Bin; Bluestein, Barry I.

doi:10.1016/j.cccn.2003.12.025

Cited by 17 publications

(13 citation statements)

References 17 publications

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“…A summary of different techniques is shown below. It has been reported by Shipchandler & Moore, (1995) and Frantzen et al, (1998) that tHcy' can be measured through immunoassay techniques, such as the Abbott IMx (fluorescence polarization) analyser or the Bayer ADVIA Centaur® chemiluminescent assay (Tewari et al, 2004). Both these assays use a mouse monoclonal antibody directed against S-adenosylhomocysteine, which is formed when Hcy is allowed to react with adenosine in the presence of S-adenosylhomocysteine hydrolase.…”

Section: Methodologiesmentioning

confidence: 99%

An evaluation of plasma homocysteine in the assessment of vitamin B₁₂status of pasture-fed sheep

Furlong

Sedcole

Sykes

2010

New Zealand Veterinary Journal

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of a thesis submitted in partial fulfilment of the requirements for the Degree ofM.Appl.Sc. Improvement of HPLC methodology was carried out to resolve peak integrity, precision, run time and stability issues encountered previously in the assay ofHcy. An Evaluation ofEighty two pregnant ewes, half of which were supplemented with a Co bullet, were grazed together on a Southland property known to be Co deficient. Half of the lambs from each group were supplemented with 3mg microencapsulated Cbl (SMARTShot™) one month after lambing (day 0), providing four groups. Lambs were blood sampled and weighed, at monthly intervals, until approximately 210 days of age and were weaned at day 89.A 40% difference in mean liveweight was recorded between the supplemented and unsupplemented lamb groups. Mean monthly total homocysteine (tHcy) concentration ranged from 1.5 to 4.5 ~mol/l. On day 0 tHey concentrations in lambs from supplemented 11 and unsupplemented ewe groups were 4.0 and 2.2 /lmol/l; a difference which was significant (p <0.001). Subsequently there were no differences between unsupplemented and supplemented lamb groups.This lack of effect occurred despite plasma concentrations of MMA and Cbl at which response to supplementation could be expected and L WG responses to Cbl supplementation were observed. Unsupplemented lamb MMA and Cbl concentrations (> 16 /lmol/l and <170 pmol/l, respectively, compared to the deficient status thresholds from reference ranges of > 13 /lmol/l and <200 pmol/l respectively) were attained on day 57. No correlation between Hcy and MMA or Cbl concentrations was detected even in individual animals whose performance was severely compromised by deficiency. This suggested that the biological importance of the Hcy pathway means that it is highly conserved, even in extreme deficiency.To purSue this further, data 'from a previous supplementation trial in which 34 individual unsupplemented animals had been removed due to welfare issues, due to severe ill-health, were examined. Mean tHcy concentrations ranging from 6 -13 /lmolll were recorded, in association with Cbl concentrations below 200 pmol/l throughout and steady rise in MMA concentration to very high values (from 10 -26 /lmol/l). The severity of the deficiency at onset was characterised by rapid response to supplementation although tHcy showed no associated change as a consequence.In conclusion, the measurement of tHcy as a metabolic indicator of Cbl deficiencyIn sheep on typical New Zealand pasture appears to have no value in detecting the incidence or assessment of the severity of the disease. The possibility that diet and other factors such as alternative pathways, including the betaine -methionine metabolic pathways, may regulate and conserve methylation pathways is discussed, but a conclusion that MMA is the first rate limiting pathway and the metabolic indicator of choice in predicting the clinical Cbl status in sheep, seems most plausible.

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Section: Methodologiesmentioning

confidence: 99%

An evaluation of plasma homocysteine in the assessment of vitamin B₁₂status of pasture-fed sheep

Furlong

Sedcole

Sykes

2010

New Zealand Veterinary Journal

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“…The chemiluminescent reaction is initiated by the addition of alkaline hydrogen peroxide to the washed PMP. Chemiluminescence is measured by a luminometer as relative light units [17].…”

Section: Plasma Analysesmentioning

confidence: 99%

Chinese Yellow Wine and Red Wine Inhibit Matrix Metalloproteinase‐2 and Improve Atherosclerotic Plaque in LDL Receptor Knockout Mice

Guo

Liu

Shi

et al. 2010

Cardiovascular Therapeutics

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Our previous study found that Chinese yellow wine could inhibit the production of homocysteine (HCY) induced extracellular matrix metalloproteinase‐2 (MMP‐2) in the cultured rat vascular smooth muscle cells. Little is known about the relationship between Chinese yellow wine and atherosclerosis or MMP‐2 in vivo. Thirty‐two LDL Receptor knockout mice on a high‐fat and l‐methionine diet developed plasma hyperhomocysteinemia and atherosclerosis. They were randomly divided into yellow wine group (n = 8), red wine group (n = 8), ethanol group (n = 8), and control group (n = 8), they were sacrificed after 14 weeks. There were no significant differences with plasma total cholesterol (TC), triglyceride (TG), high‐density lipoprotein cholesterol (HDL‐C), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) levels in the four groups. Plasma HCY was significantly decreased in the yellow wine group compared to the other three groups (P < 0.01). Yellow wine and red wine groups significantly reduced the atherosclerosis lesion area compared to ethanol and control groups (P < 0.001). However, there was no significant discrepancy between the yellow wine group and red wine group. Compared to the control group and ethanol group, the production of MMP‐2 reduced 26.8% and 23.6% in the aortic sinus and the activation of MMP‐2 reduced 32.6% and 27.3% in the aortic arch in the yellow wine group; the production of MMP‐2 reduced 25.7% and 22.4% in the aortic sinus and the activation of MMP‐2 reduced 30.2% and 26.6% in the aortic arch in the red wine group. These results suggest that Chinese yellow wine and red wine can inhibit MMP‐2 and improve atherosclerosis, and maybe both Chinese yellow wine and red wine have beneficial effects on cardiovascular disease by inhibiting MMP‐2.

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“…Plasma homocysteine was assessed using a well-established and validated chemiluminescence immunoassay (Bayer ADVIA Centaur Assay, Australia) [11]. Concentrations of plasma hsCRP were determined using a validated immuno-turbidimetric assay (Olympus OSR6199 Analyzer, Australia) [12].…”

Section: Methodsmentioning

confidence: 99%

Endothelial Function, Oxidative Stress and Inflammatory Studies in Chronic Coronary Slow Flow Phenomenon Patients

et al. 2012

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The coronary slow flow phenomenon (CSFP) is associated with coronary microvascular dysfunction although the responsible mechanisms are unknown. This study compared endothelial function assessed by changes in augmentation index (AIx) following endothelium-independent (glyceryl trinitrate, GTN) and endothelium-dependent vasodilators (salbutamol), in 40 stable CSFP patients and 23 age-matched healthy controls. Plasma concentrations of inflammatory proteins (myeloperoxidase and high-sensitivity C-reactive protein), oxidative stress biomarkers (malondialdehyde and homocysteine), and asymmetric dimethylarginine levels were also determined. There were no differences between CSFP and controls in response to salbutamol (AIx: –2.28 ± 0.88% vs. –3.22 ± 0.70%, p = 0.4) or GTN (AIx: –11.30 ± 0.75% vs. –13.30 ± 1.00%, p = 0.12). Similarly, there were no differences in the measured biomarkers. Thus, alternate mechanisms to the assessed endothelial function, inflammatory and oxidative stress processes should be explored to explain the microvascular dysfunction in CSFP patients.

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Analytical and clinical evaluation of the Bayer ADVIA Centaur® homocysteine assay

Cited by 17 publications

References 17 publications

An evaluation of plasma homocysteine in the assessment of vitamin B₁₂status of pasture-fed sheep

An evaluation of plasma homocysteine in the assessment of vitamin B₁₂status of pasture-fed sheep

Chinese Yellow Wine and Red Wine Inhibit Matrix Metalloproteinase‐2 and Improve Atherosclerotic Plaque in LDL Receptor Knockout Mice

Endothelial Function, Oxidative Stress and Inflammatory Studies in Chronic Coronary Slow Flow Phenomenon Patients

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Analytical and clinical evaluation of the Bayer ADVIA Centaur® homocysteine assay

Cited by 17 publications

References 17 publications

An evaluation of plasma homocysteine in the assessment of vitamin B12status of pasture-fed sheep

An evaluation of plasma homocysteine in the assessment of vitamin B12status of pasture-fed sheep

Chinese Yellow Wine and Red Wine Inhibit Matrix Metalloproteinase‐2 and Improve Atherosclerotic Plaque in LDL Receptor Knockout Mice

Endothelial Function, Oxidative Stress and Inflammatory Studies in Chronic Coronary Slow Flow Phenomenon Patients

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An evaluation of plasma homocysteine in the assessment of vitamin B₁₂status of pasture-fed sheep

An evaluation of plasma homocysteine in the assessment of vitamin B₁₂status of pasture-fed sheep