Analytical Applications of Nanoparticles in Maldi-MS for Bioanalysis

Kailasa, Suresh Kumar; D'souza, Stephanie L.; Wu, Hui‐Fen

doi:10.4155/bio.15.149

Cited by 11 publications

(3 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This sheds light on the enormous sensitivities of modern MS detectors. The application of non-conventional matrices is a developing field and seems to be particularly relevant to the field of MSI studies [117].…”

Section: Which Matrix Fits Which Lipid Class?mentioning

confidence: 99%

Recent Developments of Useful MALDI Matrices for the Mass Spectrometric Characterization of Lipids

et al. 2018

View full text Add to dashboard Cite

Matrix-assisted laser desorption/ionization (MALDI) is one of the most successful “soft” ionization methods in the field of mass spectrometry and enables the analysis of a broad range of molecules, including lipids. Although the details of the ionization process are still unknown, the importance of the matrix is commonly accepted. Both, the development of and the search for useful matrices was, and still is, an empirical process, since properties like vacuum stability, high absorption at the laser wavelength, etc. have to be fulfilled by a compound to become a useful matrix. This review provides a survey of successfully used MALDI matrices for the lipid analyses of complex biological samples. The advantages and drawbacks of the established organic matrix molecules (cinnamic or benzoic acid derivatives), liquid crystalline matrices, and mixtures of common matrices will be discussed. Furthermore, we will deal with nanocrystalline matrices, which are most suitable to analyze small molecules, such as free fatty acids. It will be shown that the analysis of mixtures and the quantitative analysis of small molecules can be easily performed if the matrix is carefully selected. Finally, some basic principles of how useful matrix compounds can be “designed” de novo will be introduced.

show abstract

Section: Which Matrix Fits Which Lipid Class?mentioning

confidence: 99%

Recent Developments of Useful MALDI Matrices for the Mass Spectrometric Characterization of Lipids

et al. 2018

View full text Add to dashboard Cite

show abstract

“…49 In recent years, the importance of nanomaterials as outstanding MALDI matrices has become increasingly evident due to their unique physicochemical characteristics, such as excellent optical absorption, exceptional electrical properties, and low background interference. [50][51][52][53][54][55][56][57] Among these materials, carbon-based nanomaterials such as carbon nanotubes, 58 carbon nanodots, 59,60 carbon nanoparticles (CNPs), 50,61 and graphene nanosheets (GNs) 50,62,63 have attracted the most attention. Compared to traditional organic matrices, these nanomaterials facilitate the desorption process and enhance MS signal intensity due to their superior interaction with biomolecules and efficient energy absorption.…”

Section: Introductionmentioning

confidence: 99%

Characterization of glycan isomers using magnetic carbon nanoparticles as a MALDI co-matrix

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Although nanoparticles such as gold, titanium dioxide, carbon nanotubes and zinc oxide have been used as inorganic matrix materials for MALDI and SALDI MS (surface assisted laser desorption ionization mass spectrometry) analysis for many years 18 19 20 21 22 , research efforts have been focused only on their roles as energy mediators. It has been widely accepted that nanoparticles have high surface areas, high photo absorption capabilities and can effectively transfer photo energy to surrounding analyze molecules, which are eventually ionized and detected in positive ion mode of mass spectrometers 23 24 25 . This work is aimed to demonstrate the roles of photo-generated electrons in ionization of biological molecules.…”

mentioning

confidence: 99%

Imaging of Endogenous Metabolites of Plant Leaves by Mass Spectrometry Based on Laser Activated Electron Tunneling

Huang

Tang

Zhang

et al. 2016

Sci Rep

View full text Add to dashboard Cite

A new mass spectrometric imaging approach based on laser activated electron tunneling (LAET) was described and applied to analysis of endogenous metabolites of plant leaves. LAET is an electron-directed soft ionization technique. Compressed thin films of semiconductor nanoparticles of bismuth cobalt zinc oxide were placed on the sample plate for proof-of-principle demonstration because they can not only absorb ultraviolet laser but also have high electron mobility. Upon laser irradiation, electrons are excited from valence bands to conduction bands. With appropriate kinetic energies, photoexcited electrons can tunnel away from the barrier and eventually be captured by charge deficient atoms present in neutral molecules. Resultant unpaired electron subsequently initiates specific chemical bond cleavage and generates ions that can be detected in negative ion mode of the mass spectrometer. LAET avoids the co-crystallization process of routinely used organic matrix materials with analyzes in MALDI (matrix assisted-laser desorption ionization) analysis. Thus uneven distribution of crystals with different sizes and shapes as well as background peaks in the low mass range resulting from matrix molecules is eliminated. Advantages of LAET imaging technique include not only improved spatial resolution but also photoelectron capture dissociation which produces predictable fragment ions.

show abstract

Analytical Applications of Nanoparticles in Maldi-MS for Bioanalysis

Cited by 11 publications

References 43 publications

Recent Developments of Useful MALDI Matrices for the Mass Spectrometric Characterization of Lipids

Recent Developments of Useful MALDI Matrices for the Mass Spectrometric Characterization of Lipids

Characterization of glycan isomers using magnetic carbon nanoparticles as a MALDI co-matrix

Imaging of Endogenous Metabolites of Plant Leaves by Mass Spectrometry Based on Laser Activated Electron Tunneling

Contact Info

Product

Resources

About