Analytical approaches to determination of total choline in foods and dietary supplements

Phillips, Melissa M.

doi:10.1007/s00216-011-5652-5

Cited by 38 publications

(31 citation statements)

References 32 publications

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“…In cases of choline insufficiency, diseases such as liver fattening, renal impairment, and even cancer may emerge (Garrow, 2007). Studies have shown that problems in liver, kidneys, and pancreas and disorders of memory and growth might be encountered in chronic choline insufficiency (Phillips, 2012 During this study, quail meat rich in choline, which is very beneficial considering healthy nutrition, was obtained, thus allowing people to take advantage of it. In the literature, there is no previous research related to quail meat rich in choline.…”

Section: Discussionmentioning

confidence: 99%

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Effects of supplementary choline on quail meat and fatty liver

2017

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-The objective of this study was to investigate the effects of supplementary choline on amount of choline passing to quail meat and reduction of fat deposition in liver. A hundred and thirty-six one-day-old Japanese quails were divided into eight equal groups of 17 quails each. The experiment was designed based on completely randomized design in two controls and six treatment groups: high stocking density, basal diet, control group (HSD; control 1); normal stocking density, basal diet, control group (NSD; control 2); HSD, basal diet + 900 mg kg −1 choline (A); NSD, basal diet + 900 mg kg −1 choline (B); HSD, basal diet + 1800 mg kg −1 choline (C); NSD, basal diet + 1800 mg kg −1 choline (D); HSD, basal diet + 3600 mg kg −1 choline (E); and NSD, basal diet + 3600 mg kg −1 choline (F). Feeds and water were supplied ad libitum. Continuous light was provided. Quails were kept in cages of 50 × 100 cm in size. The choline content of quail meat increased with increasing amount of added choline to feed. The highest choline content (439.2 and 561.2 mg kg −1 ) in quail meat was detected in E and F groups which fed the 3600 mg kg −1 choline supplemented diet. The total lipid content of liver in E and F groups was reduced by 42% compared with the control groups. We recommend the use of supplementary choline that can improve the amount of choline passing to quail meat and reduction of fat deposition in liver.

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Section: Discussionmentioning

confidence: 99%

“…It enables the transportation and burning of fats, which are also related to its preventive effect in fatty liver. In experimental animals, choline insufficiency caused liver fattening and loss of hepatocytes, atherosclerosis and heart diseases, growth and bone development anomalies, and impairment in kidney functions (Phillips, 2012).…”

Section: Introductionmentioning

confidence: 99%

Effects of supplementary choline on quail meat and fatty liver

2017

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“…Ch is not electroactive; it lacks fluorescence and does not absorb UV radiation. Determination of Ch can be performed by using microbiological assays, gas chromatography [5], capillary zone electrophoresis [6], and high performance liquid chromatography [7] (see the review [8]). The above-mentioned methods provide good repeatability, high sensitivity, and low limit of detection.…”

Section: Introductionmentioning

confidence: 99%

Amperometric Biosensor Based on Enzymatic Reactor for Choline Determination in Flow Systems

2019

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A simple, selective and stable biosensor with the enzymatic reactor based on choline oxidase (ChOx) was developed and applied for the determination of choline (Ch) in flow injection analysis with amperometric detection. The enzyme ChOx was covalently immobilized with glutaraldehyde to mesoporous silica powder (SBA‐15) previously covered by NH2‐groups. This powder was found as an optimal filling of the reactor. The detection of Ch is based on amperometric monitoring of consumed oxygen during the enzymatic reaction, which is directly proportional to Ch concentration. Two arrangements of an electrolytic cell in FIA, namely wall‐jet cell with working silver solid amalgam electrode covered by mercury film and flow‐through cell with tubular detector of polished silver solid amalgam were compared. The experimental parameters affecting the sensitivity and stability of the biosensor (i. e. pH of the carrier solution, volume of reactor, amount of the immobilized enzyme, the detection potential, flow rate, etc.) were optimized. Under the optimized conditions, the limit of detection was found to be 9.0×10−6 mol L−1. The Michaelis‐Menten constant for covalently immobilized ChOx on SBA‐15 was calculated. The proposed amperometric biosensor with the developed ChOx‐based reactor exhibits good repeatability, reproducibility, long‐term stability, and reusability. Its efficiency has been confirmed by the successful application for the determination of Ch in two commercial pharmaceuticals.

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“…Since choline that synthesized by the organism is limited, the additional quantities of choline need to be obtained from vitamin formula, infant food and sport drinks. Choline has become a commonly food additive which was widely used in our daily life ,. Choline is now considered as a safe food additive.…”

Section: Introductionmentioning

confidence: 99%

A Highly Sensitive Electrochemiluminescence Choline Biosensor Based on Poly(aniline‐luminol‐hemin) Nanocomposites

et al. 2019

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Poly(aniline‐luminol‐hemin) nanocomposites are prepared on an electrode surface through electropolymerization, and a highly sensitive electrochemiluminescence (ECL) biosensor for choline is developed based on the poly(aniline‐luminol‐hemin) nanocomposites and an enzyme catalyzed reaction of choline oxidase (CHOD). The obtained nanocomposites are characterized by scanning electron microscopy (SEM), atomic absorption spectrometry (AAS) and ECL. The results indicate that hemin can be incorporated into the poly(aniline‐luminol) nanocomposites using the facile electropolymerization method, and the poly(aniline‐luminol‐hemin) nanocomposites are rod shaped porous nanostructure. Moreover, the poly(aniline‐luminol‐hemin) nanocomposites exhibit higher ECL intensity than poly(aniline‐luminol) nanocomposites in alkaline media due to the catalytic effect of hemin on the ECL of the polymerized luminol and the electron transfer ability of hemin in the nanocomposites. CHOD is immobilized on the surface of the poly(aniline‐luminol‐hemin) nanocomposites modified electrode with glutaraldehyde, and the ECL biosensor based on poly(aniline‐luminol‐hemin)/CHOD exhibits a wider linear range for the choline detection. The enhanced ECL signals are linear with the logarithm of concentration of choline over the range of 1.0×10−11∼1.0×10−7 mol L−1 with a low detection limit of 1.2×10−12 mol L−1. Moreover, the proposed biosensor is successfully applied to the detection of choline in milk.

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Analytical approaches to determination of total choline in foods and dietary supplements

Cited by 38 publications

References 32 publications

Effects of supplementary choline on quail meat and fatty liver

Effects of supplementary choline on quail meat and fatty liver

Amperometric Biosensor Based on Enzymatic Reactor for Choline Determination in Flow Systems

A Highly Sensitive Electrochemiluminescence Choline Biosensor Based on Poly(aniline‐luminol‐hemin) Nanocomposites

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