Analytical Challenges and Recent Advances in the Determination of Estrogens in Water Environments

Briciu, Rodica Domnica; Kot‐Wasik, Agata; Namieśnik, Jacek

doi:10.1093/chromsci/47.2.127

Cited by 50 publications

(26 citation statements)

References 180 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Nowadays the main analytical tools are liquid and gas chromatographic techniques, mostly combined with mass spectrometric analysis. For detailed information on current analytical techniques for the detection of aquatic pollutants, the reader is referred to reviews in this field ([12–18] and the references therein). The analysis of antibacterials, for example, in environmental waters is usually performed by HPLC-MS or HPLC-MS/MS.…”

Section: Biosensor Application To Detect Antibacterials In Environmenmentioning

confidence: 99%

Biosensor Applications in the Field of Antibiotic Research—A Review of Recent Developments

Reder-Christ

Bendas

2011

Sensors

View full text Add to dashboard Cite

Antibacterials are among of the most important medications used in health care. However, their efficacy is increasingly impeded by a tremendous and globally spread bacterial resistance phenomenon. This bacterial resistance is accelerated by inadequate application of antibacterial drugs in humans, the widespread veterinary use of antibacterials, and antibacterial occurrence in the environment and food. Further, there is a lack of development of innovative novel drugs. Therefore, the search for novel antibacterials has to be intensified and the spread of antibacterials in the environment has to be restricted. Due to the fundamental progress in biosensor development and promising applications in the antibiotic field, this review gives for the first time an overview on the use and prospects of biosensor applications in that area. A number of reports have applied biosensors of different design and techniques to search for antibacterials in environmental and foodstuff matrices. These studies are discussed with respect to the analytical values and compared to conventional techniques. Furthermore, biosensor applications to elucidate the mode of action of antimicrobial drugs in vitro have been described. These studies were critically introduced referring to the informational value of those simulations. In summary, biosensors will be illustrated as an innovative and promising, although not yet comprehensively applied, technique in the antibacterial field.

show abstract

Section: Biosensor Application To Detect Antibacterials In Environmenmentioning

confidence: 99%

Biosensor Applications in the Field of Antibiotic Research—A Review of Recent Developments

Reder-Christ

Bendas

2011

Sensors

View full text Add to dashboard Cite

show abstract

“…São representados por alguns integrantes dos seguintes grupos: produtos farmacêuticos, produtos de higiene pessoal, surfactantes, agrotóxicos, aditivos industriais, dentre outros produtos químicos (Bolong et al 2009 No que se refere a identificação e quantificação desses contaminantes em matrizes ambientais, o fato de serem encontrados em baixas quantidades (ng L -1 ) faz com que a maior parte dos equipamentos não consiga detectá-los (Ghiselli & Jardim 2007). Isso, somado à complexidade das amostras ambientais e ao fato de que os IEs, incluindo os estrógenos, podem sofrer biodegradação e fotodegradação durante o transporte e a estocagem (Dionísio et al 2010), faz com que seja necessário o emprego criterioso de técnicas adequadas de preservação, purificação e extração das amostras, além do uso de equipamentos com baixos limites de detecção (Briciu et al 2009). Logo, o preparo da amostra antes da análise instrumental é um requisito essencial para o estabelecimento de um método analítico sensível e seletivo.…”

Section: Introductionunclassified

“…Logo, o preparo da amostra antes da análise instrumental é um requisito essencial para o estabelecimento de um método analítico sensível e seletivo. Briciu et al (2009) destacam que a melhor estratégia para preservação de amostras aquosas para análise de contaminantes em quantidades traço, como os estrógenos, consiste em passar a amostra por um cartucho de extração contendo uma fase sólida adsorvedora, eluir os analitos de interesse com solvente apropriado e prosseguir com a preservação do extrato a -18°C. Sob estas condições, facilita-se o armazenamento e não se observa perda significativa após 60 dias.…”

Section: Introductionunclassified

Quantificação de substâncias com atividade estrogênica e caracterização da toxicidade embriolarval em zebrafish (Danio rerio) nos efluentes das estações de tratamento de esgotos sul e norte e no Lago Paranoá, Brasília-DF

Silva¹

View full text Add to dashboard Cite

Sewage release results in the disposal of various pollutants in water bodies, including the so-called emerging pollutants. Among these are the endocrine disrupters, exogenous agents that mimic the natural hormones binding to cell receptor sites activating or blocking cell signaling pathways that trigger hormone function. These pollutants have been found in environmental samples all over the world, including Brazil, at low concentrations causing effects to exposed organisms. In this context, the aims of this study were to investigate the occurrence of three emerging pollutants with estrogenic activity (17β-estradiol-E2, 17α-ethinylestradiol-EE2 and bisphenol A-BPA) in water samples from Paranoá Lake and effluent of Sewage Treatment Plants (STPs), North and South, in Brasília-DF; Evaluate the toxicity of both environmental samples as the pollutant most frequently detected (BPA) to zebrafish embryonic development. In addition, it was tested, in the same animal model, sub lethal doses of BPA by biochemical markers. Finally, the environmental concentrations detected were compared with effect concentrations, described in literature, for aquatic animals from the first and second trophic levels. In the quantification analysis, samples were processed by solid phase extraction (SPE) and subjected to an analytical method developed in HPLC/DAD/FL. In this case, the mobile phase was acetonitrile with water in the ratio 40:60 (v/v), acidified with trifluoroacetic acid (0,12%) in isocratic condition. All the toxicity assays was performed following the OECD Protocol 236 (Guideline on Fish Embryo Toxicity Test-FET). The results showed that BPA was the most predominant pollutant in analyzed samples, with 100% of detection in the treated wastewater and 23% in surface water. The concentrations found were between <394-1231 ng L-1 in the effluent and <197-644 ng L-1 in Lake samples. Other compounds, E2 and EE2, showed low frequency of detection (<18%) in both Lake and effluent samples and, for only one effluent sample, EE2 was quantified in concentration of 591 ng L-1 .Toxicity tests showed that water samples from Paranoá Lake and South STP effluent were no toxic to zebrafish embryos; BPA is slightly toxic to zebrafish embryos (LC50 13.45 mg L-1) and was rapidly degraded in an aqueous medium (4-5 days). However, effects on hatching (CEO = 10 mg L-1), delayed pigmentation, craniofacial formation (CEO = 21.54 mg L-1) and inhibition of GST (CEO = 11.68 ug L-1) were observed in embryos exposed to BPA. In addition, the levels at which the three pollutants were found in the analyzed samples are among the effects concentrations reported in the literature for aquatic animals of the first and second trophic levels, with the exception of BPA for primary consumers. Moreover, the literature suggests that BPA tends to be less toxic than E2 and EE2. It follows that, despite being the most often detected in samples, BPA is the one with less toxicity to aquatic animals. This data contribute to enlarge the database related to the presence of contamina...

show abstract

“…They are a family of polycyclic ring structure chemicals that contains a common molecular framework [5]. The presence of EDCs in water bodies is contributed mainly by human and animal excretions as glucuronides and sulphates, which are the inactive polar conjugates [6], [7].…”

Section: Introduction According To the United States (Us) Environmmentioning

confidence: 99%

Isolation and Identification of 17α- Ethinylestradiol — Degrading Bacterial Strains from POME and Cow Dung

Lim¹,

Morad²,

Lalung³

et al. 2016

IJESD

View full text Add to dashboard Cite

Abstract-Theisolation of 17α-Ethinylestradiol (EE2)-degrading bacterial strains was done using samples sourced from Palm Oil Mill Effluent (POME) and fresh cow dung. Screening processes were conducted to identify the potential strains. The three highest potential EE2-degrading bacterial strains obtained from each sample source were selected for further batch experiments. A batch study with the strains fed with only salt medium containing EE2 as the sole carbon source was conducted for 9 days. The bacterial strain isolated from POME that was capable of degrading EE2 of up to 64.0 % at EE2 concentration of 5 mg/L was identified as Hyphomicrobium facile. The strain isolated from the cow faeces that was capable of degrading EE2 up to 55.1 % at concentration of 10 mg/L was identified as Enterobacter cloacae.Index Terms-17α-ethinylestradiol, cow dung, Enterobacter cloacae, Hyphomicrobium facile, palm oil mill effluent.

show abstract

Analytical Challenges and Recent Advances in the Determination of Estrogens in Water Environments

Cited by 50 publications

References 180 publications

Biosensor Applications in the Field of Antibiotic Research—A Review of Recent Developments

Biosensor Applications in the Field of Antibiotic Research—A Review of Recent Developments

Quantificação de substâncias com atividade estrogênica e caracterização da toxicidade embriolarval em zebrafish (Danio rerio) nos efluentes das estações de tratamento de esgotos sul e norte e no Lago Paranoá, Brasília-DF

Isolation and Identification of 17α- Ethinylestradiol — Degrading Bacterial Strains from POME and Cow Dung

Contact Info

Product

Resources

About