Analytical evaluation of the new Abbott Architect 25-OH vitamin D assay

Cavalier, Étienne; Carlisi, Agnès; Bekaert, Anne-Catherine; Rousselle, Olivier; Chapelle, Jean-Paul; Souberbielle, Jean‐Claude

doi:10.1016/j.clinbiochem.2012.01.021

Cited by 20 publications

(12 citation statements)

References 8 publications

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“…These findings obtained for the whole group showed variable concordance with other studies and were compatible with recent studies on the performance of the Architect and Cobas systems. 35,36 These regression analysis results were also comparable with the results reported by Ong et al In another comparison study conducted by Farrel et al, both the Architect and the Cobas systems exhibited dissimilar results. 37,38 Heijboer et al evaluated the influence of DBP on the accuracy of the assays and found better results for the Cobas system and worse results for the Architect system using healthy individuals.…”

Section: Discussionsupporting

confidence: 81%

Evaluation of Vitamin D Assays in Relation to the Measurement Range and the Statistical Method

Yücel¹,

Kara²,

Madenci³

et al. 2014

Turkiye Klinikleri J Med Sci

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A AB BS S T TR RA AC CT T O Ob bj je ec ct ti iv ve e: : The aim of this study was to compare three vitamin D measuring assays with a reference method to analyze whether their performance varied according to different statistical procedures and measurement ranges used. M Ma at te er ri ia al l a an nd d M Me et th ho od ds s: : Three vitamin D measurement methods, a high-performance liquid chromatography (HPLC) and two immunoassay systems (Architect, Abbott Diagnostics and Cobas, Roche Diagnostics) were compared with liquid chromatography tandem mass spectrometry (LCMS/MS) as the reference method in a study population of 141 individuals. The study group was investigated as whole group and two different subgroups of <50 ng/ml and <20 ng/ml, according to LCMS/MS measurements. The results were analyzed by Passing Bablok regression analysis, concordance correlation coefficient, interrater agreement, Bland Altman plots. R Re es su ul lt ts s: : Data generated from the various statistical analyses revealed that all of the methods investigated in this study performed worse for lower concentrations (<50 ng/ml and <20 ng/ml). The best overall performance was obtained by HPLC method. The methods showed variable performance with the statistical analysis used. C Co on nc cl lu us si io on n: : Method comparison studies of vitamin D are prone to variable results according to the statistical method and measurement range chosen. Method comparison studies should be based on clinical requirements. Laboratory professionals should determine the range that is important from clinical point of view, and should evaluate the results according to total allowable error for that range.K Ke ey y W Wo or rd ds s: : Vitamin D; immunoassay; chromatography, high pressure liquid; mass spectrometry Ö ÖZ ZE ET T A Am ma aç ç: : Çalışmanın amacı üç D vitamini ölçüm yöntemini, referans kabul edilen bir yöntemle karşılaştırmak ve performanslarının kullanılan istatistiksel yöntem ve seçilen ölçüm aralığına göre değişim gösterip göstermediğini değerlendirmektir. G Ge er re eç ç v ve e Y Yö ön nt te em ml le er r: : Yüz kırk bir bireyden oluşan bir çalışma grubunda, bir yüksek performans likid kromatografi (HPLC) ve iki immunoassay yöntemi (Architect, Abbott Diagnostics ve Cobas, Roche Diagnostics) referans yöntem olarak alınan bir sıvı kromatografi-ardşık kuẗle spektrometresi (LCMS/MS) yöntemi ile karşılaştırıldı. Sonuçlar tüm çalışma grubu, D vitamini konsantrasyonu <50 ng/ml olan ve <20 ng/ml olan üç ayrı alt grupta incelendi. Elde edilen veriler Passing Bablok regresyon analizi, konkordans uyum katsayısı, interrater uyum, Bland Altman grafikleri ile değerlendirildi. B Bu ul lg gu ul la ar r: : Farklı istatistiksel analizlerin sonucuna göre, tüm yöntemler daha düşük konsantrasyonlarda (<50 ng/ml ve <20 ng/ml) daha düşük performans gösterdi. Ayrıca yöntemler, kullanılan istatistiksel analize bağlı olarak değişen performanslar sergilediler. En iyi toplam performans HPLC yöntemi ile elde edildi. S So on nu uç ç: : Yöntem karşılaştırma çalışmalarında el...

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Section: Discussionsupporting

confidence: 81%

Evaluation of Vitamin D Assays in Relation to the Measurement Range and the Statistical Method

Yücel¹,

Kara²,

Madenci³

et al. 2014

Turkiye Klinikleri J Med Sci

View full text Add to dashboard Cite

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“…Subjects were also categorized as vitamin D sufficient or deficient, as defined by a 25(OH)D level below 20 ng/mL (14). The vitamin D assays (DiaSorin and Abbott) have good correlation at 25(OH)D levels of less than 50 ng/mL, but the Abbott assay has systemic positive bias compared to DiaSorin at levels above than 50 ng/mL (15). Therefore, this bias was avoided by introducing the above categories.…”

Section: Discussionmentioning

confidence: 99%

Trends in bone mineral density in young adults with cystic fibrosis over a 15 year period

Putman

Baker

Uluer

et al. 2015

Journal of Cystic Fibrosis

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Background Improvements in clinical care have led to increased life expectancy in patients with cystic fibrosis (CF) over the past several decades. Whether these improvements have had significant effects on bone health in patients with CF is unclear. Methods This is a cross-sectional study comparing clinical characteristics and bone mineral density (BMD) measured by dual energy X-ray absorptiometry (DXA) in adults with CF evaluated in 1995–1999 to age-, race-, and gender matched patients with CF evaluated in 2011–2013 at the same center on calibrated DXA machines. Results The cohorts were similar in terms of age, BMI, pancreatic insufficiency, presence of F508del mutation, and reproductive history. In the most recent cohort, pulmonary function was superior, and fewer patients had vitamin D deficiency or secondary hyperparathyroidism. Areal BMD measures of the PA spine, lateral spine, and distal radius were similarly low in the two cohorts. Conclusions Although pulmonary function and vitamin D status were better in patients in the present-day cohort, areal BMD of the spine was reduced in a significant number of patients and was no different in patients with CF today than in the late 1990s. Further attention to optimizing bone health may be necessary to prevent CF-related bone disease.

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“…25(OH)D is the clinical indicator of vitamin D status and the precursor of the most biologically active form of vitamin D, 1,25-dihydroxyvitamin D. Maternal plasma 25-hydroxyvitamin D (25(OH)D) levels were measured in the repository sample with an Abbott Architect chemiluminescent microparticle immunoassay [10] on an Architect i2000SR analyzer (Abbott, Abbott Park, IL, USA), according to the manufacturer's instructions, at ARUP Laboratories (Salt Lake City, UT, USA). The samples, which had been previously processed and stored in aliquots at –80°C in a central repository, were shipped frozen and maintained below –80°C until the time that the vitamin D assay was performed.…”

Section: Methodsmentioning

confidence: 99%

Low vitamin D status among pregnant Latin American and Caribbean women with HIV Infection

Jao

Freimanis

Mussi-Pinhata

et al. 2015

Intl J Gynecology & Obste

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Objective To evaluate the prevalence and predictors of low vitamin D status among pregnant women with HIV infection. Methods The present cross-sectional study analyzed repository specimens collected at 12–34 weeks of pregnancy among women enrolled in across 17 sites in Latin America and the Caribbean between 2002 and 2009. Logistic regression modeling was used to identify factors associated with low vitamin D status (25-hydroxyvitamin D <30 ng/mL). Results Among 715 women, 218 (30.5%) were vitamin D deficient (<20 ng/mL) and 252 (35.2%) were insufficient (21–29 ng/mL). Factors associated with low vitamin D status included residence in subtropical latitudes (adjusted odds ratio [aOR] 1.97, 95% confidence interval [CI] 1.35–2.88), assessment during non-summer seasons (autumn: aOR 1.85, 95% CI 1.20–2.86; spring: 4.3, 2.65–6.95; winter: 10.82, 5.74–20.41), employment (aOR 1.56, 95% CI 1.06–2.38), and assessment before 20 weeks of pregnancy (aOR 1.89, 95% CI 1.18–3.06). Factors protective against low vitamin D status were CD4 count below 200 cells per mm3 (aOR 0.45, 95% CI 0.26–0.77) and protease inhibitors (aOR 0.62, 95% CI 0.40–0.95). Conclusion Low vitamin D status was prevalent among pregnant women with HIV infection. Further studies are warranted to identify the impact of low maternal vitamin D status.

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Analytical evaluation of the new Abbott Architect 25-OH vitamin D assay

Cited by 20 publications

References 8 publications

Evaluation of Vitamin D Assays in Relation to the Measurement Range and the Statistical Method

Evaluation of Vitamin D Assays in Relation to the Measurement Range and the Statistical Method

Trends in bone mineral density in young adults with cystic fibrosis over a 15 year period

Low vitamin D status among pregnant Latin American and Caribbean women with HIV Infection

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