Analytical Investigation of Fluorescent Complexes of Alanine and Phenylalanine with 8-hydroxyquinaldine and 8-hydroxy quinoline in Aqueous Phase

Abstract

A new method has been developed by preparing complexes involving condensation of amino acids with 8-hydroxyquinaldine using various experimental conditions. The products so obtained are being investigated for identification and quantitative analysis using different spectroscopic techniques including fluorescence activity of newly synthesized products. In present studies the identification of amino acids in nano mole quantities has become possible by fluorometric activity of amino acids-quinaldine complex involving different excitation and emission wavelengths. This fluorometric activity of complexes is 10 to 100 times more sensitive method than assay method involving ninhydrin. 2-methy1-8-hydroxyquinoline (8-hydroxyquinaldine) condensed with Alanine and Phenylalanine produced fluorescent complexes. The complexes separately have been investigated for identification and quantitative estimation of amino acids. The use of 8-hydroxyquinaldine for various purposes and its comparison to 8-hydroxyquinoline for similar purpose indicates decrease in fluorescence signal of 8-hydroxyquinaldine-amino acid complexes.