Analytical Method by HPLC-Dad Allows Quantification of Quercetin Marker in Standardized Extract of Anadenanthera Colubrina Var. Cebil

Abstract

Objective: The Anadenanthera colubrina (Vell.) Brennan var. cebil is a medicinal plant that has been used for the treatment of many diseases in the northeastern region of Brazil. This plant contains secondary metabolites such as quercetin, a flavonoid that is known by its antioxidant and antiinflammatory effects. The aim of this work is to propose the validation of an analytical method using high-performance liquid chromatography with diode array detector (HPLC-DAD) for the quantification of quercetin and standardization of the hydroalcoholic extract (HAE) of A. colubrina. Methods:The A. colubrina extracts were prepared by the maceration process with powdered leaves at 20% weight: volume (w/v) and a hydroalcoholic solution at 50% volume: volume (v/v) for 120 h at room temperature. After pretreatment of the hydroalcoholic extract, the quercetin marker was used for quantification and proceeded to the evaluation of validation parameters for the method using HPLC-DAD. Results:The analytical method proved to be specific. Linear over the range 1.4-26.6 µg/ml, regression analysis showed a good correlation coefficient (R2= 0.999); the limit of detection (LOD) and the limit of quantification (LOQ) were 0.27 and 0.81 μg/ml respectively. The relative standard deviation (RSD) did not exceed 2.5% for precision. The proposed method was validated with an average recovery of 92.5-97.5%. Conclusion:The method was validated using HPLC-DAD, allowing the quantification of quercetin in the standardisation process of extracts and quality control of the herbal drug containing A. colubrina Phyto complex.

Development of a quantified herbal extract of hawthorn Crataegus mexicana leaves with vasodilator effect

“…Particularly, some techniques have been reported for analyzing phenolic compounds in Crataegus species ( Bardakci et al, 2019 , Hellenbrand et al, 2015 , Koch and Malek, 2011 , Orhan, 2016 , Venskutonis, 2018 ). However, every single extract plant possesses its own complexity and variations in its chemical composition, therefore, it is necessary to identify a suitable chemical marker and develop an analytical method to quantify it in order to carry out quality control analyzes of medicinal products based on it ( Souza et al, 2017 ).…”

Development and Validation of High-Performance Liquid Chromatography With Diode Array Detector Method to Analysis of Kaempferol Marker From Extracts of Poincianella Pyramidalis (Tul) L.P Queiroz

“…But the chromatographic conditions of a given method can be a good reference for the validation of another complex matrix, especially when the analytes or markers belong to the same class of secondary metabolites. Two published papers show validated methods for analyzing quercetin content in two different matrices [36,28].…”

“…The great challenge of employing an analytical method to analyze any complex samples for which the method has not been validated lies in the matrix effect that this complex sample may present through interfering substances [26][27][28].…”

Determination of quercetin aglycone in Flos Sophorae Immaturus extract of (Sophora japonica L.) by High Performance Liquid Chromatography

“…-Pha động: Trên cơ sở tỉ lệ tham khảo [16][17], chúng tôi tạo kênh C pha sẵn chứa 1% axit axetic, chứa các thành phần với % thể tích methanol/acetonitril/H 2 O lần lượt là 40/15/45.…”

“…-Đầu dò dãy diot quang (diode array detector-DAD) phát hiện quercetin: Chúng tôi tiến hành quét phổ đồng thời ở 4 bước sóng 283nm, 330nm, 367nm [7] và 370nm [17][18] để khảo sát, xác định bước sóng phù hợp cho nhận diện quercetin trong mẫu nghiên cứu.…”