Analytical methods to monitor dopamine metabolism in plasma: Moving forward with improved diagnosis and treatment of neurological disorders

Dias, Ana Sofia Abrantes; Pinto, Joana Catarina Amaral; Magalhães, Mariana; Mendes, Vera M.; Manadas, Bruno

doi:10.1016/j.jpba.2020.113323

Cited by 19 publications

(7 citation statements)

References 169 publications

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“…Tyrosine hydroxylase is the key enzyme for the synthesis of dopamine, and catalyzes tyrosine into l ‐dopa, which is then decarboxylated by l ‐dopa decarboxylase into dopamine. [ 48 ] Thus, the interaction between LEXT‐VI and Parkinson disease protein 7 homolog that activates tyrosine hydroxylase and l ‐dopa decarboxylase provides an explanation of how LETX‐VI increases the total dopamine level of PC12 cells (Figure 2). This is because Parkinson disease protein 7 homolog (short name: DJ‐1) could not only transcriptionally upregulate the tyrosine hydroxylase [ 49 ] but also directly bind to and activate tyrosine hydroxylase and l ‐dopa decarboxylase in dopaminergic cells.…”

Section: Discussionmentioning

confidence: 99%

Biochemical and cytotoxic evaluation of latroeggtoxin‐VI against PC12 cells

Tang

Wang

et al. 2021

J Biochem & Molecular Tox

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Latroeggtoxin‐VI (LETX‐VI) is a peptide neurotoxin discovered from Latrodectus tredecimguttatus eggs. In the current study, the action features of the neurotoxin on PC12 cells were systematically investigated. LETX‐VI could promote dopamine release from PC12 cells in the absence and presence of Ca2+, involving an even more complex action mechanism in the presence of Ca2+ and when the treatment time was longer. Although LETX‐VI enchanced the autophagy and secretion activity in PC 12 cells, it showed no remarkable influence on the proliferation, cell cycle, apoptosis and ultrastructure of the cells. Pulldown combined with CapLC‐MS/MS analysis suggested that LETX‐VI affected PC12 cells by interacting with multiple proteins involved in the metabolism, transport, and release of neurotransmitters, particularly dopamine. The low cytotoxicity and effective regulatory action of LETX‐VI on PC12 cells suggest the potential of the active peptide in the development of drugs for the treatment of some dopamine‐related psychotic diseases and cancers.

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Section: Discussionmentioning

confidence: 99%

Biochemical and cytotoxic evaluation of latroeggtoxin‐VI against PC12 cells

Tang

Wang

et al. 2021

J Biochem & Molecular Tox

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“…Inactivation of Parkinson disease protein 7 by small interference RNA (siRNA) results in a decreased TH expression and DDC production in human dopaminergic cell lines [20]. TH is the rate-limiting enzyme in the synthesis of dopamine, catalyzing tyrosine into L-dopa, which is decarboxylated by DDC into dopamine [14,21]. Parkinson disease is caused by loss of dopamine, which is synthesized from tyrosine by TH and DDC.…”

Section: Discussionmentioning

confidence: 99%

Pull-Down Assay-Guided Insights into the Effects of Latroeggtoxin-VI on Nerve Cells

Tang¹,

Yu²,

Wang³

et al. 2021

Toxins

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Latroeggtoxin-VI (LETX-VI) is a peptide neurotoxin newly found from the eggs of spider L. tredecimguttatus. To explore the mechanism of action of the LETX-VI on nerve cells, the effects of LETX-VI on PC12 cells, a commonly used neuron model, were analyzed using a pull-down assay-guided strategy. LETX-VI was shown to interact with 164 PC12 cell proteins that have diverse molecular functions such as binding, catalysis, regulation, structural activity, etc., thereby extensively affecting the biological processes in the PC12 cells, particularly protein metabolism, response to stimulus, substance transport, and nucleic acid metabolism, with 56.71%, 42.07%, 29.88% and 28.66% of the identified proteins being involved in these biological processes, respectively. By interacting with the relevant proteins, LETX-VI enhanced the synthesis of dopamine; positively regulated cell division and proliferation; and negatively regulated cell cycle arrest, cell death, and apoptotic processes, and therefore has limited cytotoxicity against the PC12 cells, which were further experimentally confirmed. In general, the effects of LETX-VI on PC12 cells are more regulatory than cytotoxic. These findings have deepened our understanding of the action mechanism of LETX-VI on nerve cells and provided valuable clues for further related researches including those on Parkinson’s disease.

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“…In recent years, several analytical techniques have been proposed to measure the amount of DA in humans, including fluorescence [ 7 ], calorimetry [ 8 ], liquid chromatography-mass spectrometry [ 9 ], high performance liquid chromatography-chemiluminescence [ 10 ], and capillary electrophoresis [ 11 ]. In addition, the electrochemical techniques (i.e., voltammetry, amperometry, potentiometry and electrochemical impedance spectroscopy) are the interesting alternative methods for electrochemical biosensor applications due to them being facile and low cost and having a short operation time, high sensitivity and low detection limit methods [ 12 , 13 , 14 , 15 ]. The graphene oxide-based molecular imprinted polymer (GO-MIP) was prepared for electrochemical cholesterol biosensor using cyclic voltammetry (CV).…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Dopamine Biosensor Based on Poly(3-aminobenzylamine) Layer-by-Layer Self-Assembled Multilayer Thin Film

2021

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Dopamine (DA) is an important neurotransmitter which indicates the risk of several neurological diseases. The selective determination with low detection limit is necessary for early diagnosis and prevention of neurological diseases associated with abnormal concentration of DA. The purpose of this study is to fabricate a poly(3-aminobenzylamine)/poly(sodium 4-styrenesulfonate) (PABA/PSS) multilayer thin film for use as an electrochemical DA biosensor. The PABA was firstly synthesized using a chemical oxidation method of 3-aminobenzylamine (ABA) monomer with ammonium persulfate (APS) as an oxidant. For electrochemical biosensor, the PABA/PSS thin film was fabricated on fluorine doped tin oxide (FTO)-coated glass substrate using the layer-by-layer (LBL) self-assembly method. The optimized number of bilayers was achieved using SEM and cyclic voltammetry (CV) results. The electroactivity of the optimized LBL thin film toward detection of DA in neutral solution was studied by CV and amperometry. The PABA/PSS thin film showed good sensitivity for DA sensing with sensitivity of 6.922 nA.cm−2.µM−1 and linear range of 0.1–1.0 µM (R2 = 0.9934), with low detection limit of 0.0628 µM, long-term stability and good reproducibility. In addition, the selectivity of the PABA/PSS thin film for detection of DA under the common interferences (i.e., ascorbic acid, uric acid and glucose) was also presented. The prepared PABA/PSS thin film showed the powerful efficiency for future use as DA biosensor in real sample analysis.

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Analytical methods to monitor dopamine metabolism in plasma: Moving forward with improved diagnosis and treatment of neurological disorders

Cited by 19 publications

References 169 publications

Biochemical and cytotoxic evaluation of latroeggtoxin‐VI against PC12 cells

Biochemical and cytotoxic evaluation of latroeggtoxin‐VI against PC12 cells

Pull-Down Assay-Guided Insights into the Effects of Latroeggtoxin-VI on Nerve Cells

Electrochemical Dopamine Biosensor Based on Poly(3-aminobenzylamine) Layer-by-Layer Self-Assembled Multilayer Thin Film

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