Analytical quality assurance in veterinary drug residue analysis methods: Matrix effects determination and monitoring for sulfonamides analysis

Abstract

In residue analysis of veterinary drugs in foodstuff, matrix effects are one of the most critical points. This work present a discuss considering approaches used to estimate, minimize and monitoring matrix effects in bioanalytical methods. Qualitative and quantitative methods for estimation of matrix effects such as post-column infusion, slopes ratios analysis, calibration curves (mathematical and statistical analysis) and control chart monitoring are discussed using real data. Matrix effects varying in a wide range depending of the analyte and the sample preparation method: pressurized liquid extraction for liver samples show matrix effects from 15.5 to 59.2% while a ultrasound-assisted extraction provide values from 21.7 to 64.3%. The matrix influence was also evaluated: for sulfamethazine analysis, losses of signal were varying from -37 to -96% for fish and eggs, respectively. Advantages and drawbacks are also discussed considering a workflow for matrix effects assessment proposed and applied to real data from sulfonamides residues analysis.

High-throughput method for macrolides and lincosamides antibiotics residues analysis in milk and muscle using a simple liquid–liquid extraction technique and liquid chromatography–electrospray–tandem mass spectrometry analysis (LC–MS/MS)

“…Matrix effects (ME) were estimated by the comparison between a curve prepared using matrix-matched approach and one prepared in pure solvent, as previously describe elsewhere [46][47][48]. ME was evaluated using slope ratios comparison according to the approach proposed by Romero-Gonzáles et al and Sulyok et al in a modified application of the quantitative approach of Matuszewski et al [49][50][51].…”

“…To muscle, 3 distinct species were validated (bovine, swine and poultry). To evaluate a possible matrix effect provoked by differences between tissues, a matrix-matched calibration curve plus blank samples (n ¼6) for each species were spiked at MRL level [45,48]. Calculated concentrations for each sample set were compared trough variance analysis.…”

“…As described above, the slopes ratio (RS) was used to estimate the presence of matrix effects wherein RS ¼1.0 70.1 was adopted as criteria [48][49][50][51]. To milk analysis, ERT and TYL showed RS values nearby 1.0, but all other analytes expressed a significant difference.…”

Comparison of mouse plasma and brain tissue homogenate sample pretreatment methods prior to high-performance liquid chromatography for a new 1,2,4-triazole derivative with anticonvulsant activity

“…A new synthesized 1,2,4-triazole derivative, namely 5-(3-chlorophenyl)-4-hexyl-2,4-dihydro-3H-1,2,4-triazole-3-thione was selected for the research. On the basis of previous results, this compound can be considered as a promising drug candidate with strong antiepileptic activity [22][23][24][25][26][27].…”

Desenvolvimento de métodos miniaturizados de extração em fase sólida para a pré-concentração de produtos de degradação de fluoroquinolonas e sulfonamidas em matrizes aquosas

“…Além disso, métodos de extração e condições cromatográficas devem ser otimizados e adaptados para minimizar esse efeito 55 . No caso de análises quantitativas, o uso de padrão interno ou o método de adição de padrão é altamente recomendável para uma maior confiabilidade dos resultados.…”