High-throughput method for macrolides and lincosamides antibiotics residues analysis in milk and muscle using a simple liquid–liquid extraction technique and liquid chromatography–electrospray–tandem mass spectrometry analysis (LC–MS/MS)

Jank, Louíse; Martins, Magda Targa; Arsand, Juliana Bazzan; Motta, Tanara Magalhães Campos; Hoff, Rodrigo Barcellos; Barreto, Fabiano; Pizzolato, Tânia Mara

doi:10.1016/j.talanta.2015.06.078

Cited by 93 publications

(24 citation statements)

References 46 publications

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“…Antibiotics are widely used in livestock production for many purposes, such as: animal disease treatment (therapeutic application), animal disease prevention (prophylactic application), and feed efficiency (as growing promoters) (Jank et al 2015). Their presence as residues in food products especially milk implicates certain damages in the public health like: the development of allergic reactions in some hypersensitive individuals, increased risk of carcinogenicity (Petrović et al 2008;Hou et al 2014), the growth of resistant bacterial strains, and imbalances in intestinal microflora (Wang et al 2006;Borràs et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Assessment of antibiotic residues in commercial and farm milk collected in the region of Guelma (Algeria)

Layada¹,

Benouareth²,

Coucke

et al. 2016

FoodContamination

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Background:In an attempt to enhance the quality and quantity of food production (especially milk) and in order to prevent, or treat,animal diseases, the use of antibiotics in Algeria follows an increasing trend. The increased use evidently contributes to the emergence of increased contamination levels of antibiotic residues. Results: In this work, two methods were used to detect presence of antibiotic residues in raw and fermented cow's milk collected in Guelma's farms (in Algeria). The screening comprised different points of sale in Guelma province. In a first step a widely used prescreening method based on microbial inhibition assay; Delvotest SP-NT; was used to analyze 131 milk samples. In a second step a liquid chromatography coupled to mass spectrometry (LC-MS/MS) was used. The latter was first optimized for extraction of 36 veterinary drugs of penicillins, quinolones, macrolides, tetracyclines, sulfonamides, and trimethoprim from the collected milk. After simple extraction and dilution, the 194 samples, including those previously tested by the Delvotest SP-NT, were analyzed by LC-MS/MS. Results obtained by both methods were compared. Among the LC-MS/MS findings, 65.46 % of non-conform samples contained authorized residues at levels higher than the MRL, residues without set MRL, or non-authorized residues.Conclusion: The comparison of both methods showed that Delvotest SP-NT is less trustworthy due to number of false negative results. This was further confirmed by LC-MS/MS pointing out the traces of antibiotics in numerous samples. In 65.46 % of milk samples residues of antibiotics were found suggesting a lack of public health controls as well as an evidence of the negligent use of antibiotics in the livestock industry, which both form a risk to public health. This indication should be confirmed by a nationwide study with in-depth analyses of antibiotic's presence in food chain originated from animals. The study offered an LC-MS/MS based analytical method ready to be used in Algerian National Residues Control Plan as a versatile analytical tool to monitor and determine the occurrence of antibiotic multi-residues in milk.

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Section: Introductionmentioning

confidence: 99%

Assessment of antibiotic residues in commercial and farm milk collected in the region of Guelma (Algeria)

Layada¹,

Benouareth²,

Coucke

et al. 2016

FoodContamination

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“…Several researchers investigated stabilities of numerous antibiotics such as CAP, macrolides, lincosamides, betalactams, tetracyclines and quinolones in solutions and matrices. 8,24,25 They demonstrated that the antibiotic stability depends on several factors such as the type of buffer solution and matrix as well as the temperature. According to the results of Leston et al, CAP was stabilized -20 ºC and 4 ºC approximately for 7 and 6 weeks, respectively, 8 which are in accordance with our results.…”

Section: Stability Of Cap In Aqueous Solution and Kidney Fluidmentioning

confidence: 99%

Comparative Screening of Chloramphenicol Residue in Chicken Tissues Using Four Plate Test and Premi®Test Methods

Rahimi¹,

Shahbazi²,

Ahmadi³

2018

Pharm Sci

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“…Thus, our first attempt was to use the reported Shiff base hapten strategy, but this failed to obtain an antibody against tylosin and tilmicosin because of the poor and unstable immune response. We speculated Currently, methods for simultaneous detection of tylosin and tilmicosin mainly include high-performance liquid chromatography (HPLC), high performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS), and other large-scale chromatographic methods [18][19][20]. These large-scale equipment methods are highly sensitive and accurate, but are expensive, time-consuming, have low sample throughput, and are inconvenient for detection in the field.…”

Section: Introductionmentioning

confidence: 99%

Design of Novel Haptens and Development of Monoclonal Antibody-Based Immunoassays for the Simultaneous Detection of Tylosin and Tilmicosin in Milk and Water Samples

et al. 2019

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In this work, a monoclonal antibody-based indirect competitive enzyme-linked immunosorbent assay (icELISA) was established to detect tylosin and tilmicosin in milk and water samples. A sensitive and specific monoclonal antibody was prepared by rational designed hapten, which was achieved by directly oxidizing the aldehyde group on the side chain of tylosin to the carboxyl group. Under the optimized conditions, the linear range of icELISA for tylosin and tilmicosin were 1.3 to 17.7 ng/mL and 2.0 to 47.4 ng/mL, with half-maximal inhibition concentration (IC50) values of 4.7 and 9.6 ng/mL, respectively. The cross-reactivity with other analogues of icELISA was less than 0.1%. The average recoveries of icELISA for tylosin and tilmicosin ranged from 76.4% to 109.5% in milk and water samples. Besides, the detection results of icELISA showed good correlations with HPLC-MS/MS. The proposed icELISA was satisfied for rapid and specific screening of tylosin and tilmicosin residues in milk and water samples.

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High-throughput method for macrolides and lincosamides antibiotics residues analysis in milk and muscle using a simple liquid–liquid extraction technique and liquid chromatography–electrospray–tandem mass spectrometry analysis (LC–MS/MS)

Cited by 93 publications

References 46 publications

Assessment of antibiotic residues in commercial and farm milk collected in the region of Guelma (Algeria)

Assessment of antibiotic residues in commercial and farm milk collected in the region of Guelma (Algeria)

Comparative Screening of Chloramphenicol Residue in Chicken Tissues Using Four Plate Test and Premi®Test Methods

Design of Novel Haptens and Development of Monoclonal Antibody-Based Immunoassays for the Simultaneous Detection of Tylosin and Tilmicosin in Milk and Water Samples

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