Analytical Strategies in Lipidomics for Discovery of Functional Biomarkers from Human Saliva

Agatonović-Kuštrin, Snežana; Morton, David W.; Смирнов, В. В.; Петухов, А. Е.; Gegechkori, Vladimir; Кузина, В. Н.; Gorpinchenko, Natalya; Ramenskaya, G. V.

doi:10.1155/2019/6741518

Cited by 23 publications

(22 citation statements)

References 77 publications

(81 reference statements)

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“…Thus, identification of a lipidomic signature may be relevant to detect potential diagnostic, prognostic, and predictive biomarkers in various diseases [33]. Moreover, the study of the concentrations of a subset of lipids in human disease can shed light into cellular processes and unravel alterations in one or more pathways [34].…”

Section: Introductionmentioning

confidence: 99%

Plasma Lipid Profiling Contributes to Untangle the Complexity of Moyamoya Arteriopathy

Dei

Carrozzini

Pollaci

et al. 2021

IJMS

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Moyamoya arteriopathy (MA) is a rare cerebrovascular disorder characterized by ischemic/hemorrhagic strokes. The pathophysiology is unknown. A deregulation of vasculogenic/angiogenic/inflammatory pathways has been hypothesized as a possible pathophysiological mechanism. Since lipids are implicated in modulating neo-vascularization/angiogenesis and inflammation, their deregulation is potentially involved in MA. Our aim is to evaluate angiogenic/vasculogenic/inflammatory proteins and lipid profile in plasma of MA patients and control subjects (healthy donors HD or subjects with atherosclerotic cerebrovascular disease ACVD). Angiogenic and inflammatory protein levels were measured by ELISA and a complete lipidomic analysis was performed on plasma by mass spectrometry. ELISA showed a significant decrease for MMP-9 released in plasma of MA. The untargeted lipidomic analysis showed a cumulative depletion of lipid asset in plasma of MA as compared to HD. Specifically, a decrease in membrane complex glycosphingolipids peripherally circulating in MA plasma with respect to HD was observed, likely suggestive of cerebral cellular recruitment. The quantitative targeted approach demonstrated an increase in free sphingoid bases, likely associated with a deregulated angiogenesis. Our findings indicate that lipid signature could play a central role in MA and that a detailed biomarker profile may contribute to untangle the complex, and still obscure, pathogenesis of MA.

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Section: Introductionmentioning

confidence: 99%

Plasma Lipid Profiling Contributes to Untangle the Complexity of Moyamoya Arteriopathy

Dei

Carrozzini

Pollaci

et al. 2021

IJMS

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“…In humans the composition of the saliva metabolome is a promising diagnostic tool for systemic diseases as well as oral problems, and investigation of the saliva metabolome has even resulted in the development of a specific field known as salivaomics (13). Studies in this area have yielded important information about caries, oral cancer, and systemic conditions (14)(15)(16). Due to the ease of sample collection the metabolomic study of saliva may become an important diagnostic tool in the future (17).…”

Section: Discussionmentioning

confidence: 99%

Influence of Dietary Supplementation With a Powder Containing A.N. ProDen™ (Ascophyllum Nodosum) Algae on Dog Saliva Metabolome

Gawor¹,

Wilczak

Svensson

et al. 2021

Front. Vet. Sci.

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The objective of this placebo-controlled, double-blind, randomized study (designed according to evidence-based medicine standards) was to determine the effect of 30-day administration of powdered brown algae, Ascophyllum nodosum (ProDen PlaqueOff, SwedenCare AB, Sweden), on saliva metabolomes in dogs. Sixty client-owned dogs underwent professional dental cleaning and were randomly subdivided into two groups receiving daily powdered brown algae A. nodosum, or a placebo (microcrystalline cellulose in powder), adjusted to their bodyweight. After a comprehensive oral health assessment and professional dental cleaning, which were both performed under general anesthesia, clinical assessments for gingivitis, plaque, and calculus were conducted. Saliva samples were collected at Day 0 and Day 30 of supplementation. Whole saliva is a mixed fluid that is derived predominantly from the major salivary glands but it also contains numerous other constituents. Additionally, its composition varies on whether salivary secretion is basal or stimulated. Authors put efforts to avoid contamination of saliva by other constituents and character of saliva was basal. Quadrupole time-of-flight (QTOF) mass spectrometer was used to conduct analysis of the saliva samples. Metabolomic analyses identified clear changes after 30 days of supplementation, and the direction of these changes was completely different than in dogs that received a placebo treatment during the same period. The positive clinical effect of 30 days of A. nodosum supplementation on oral health status in dogs described in previous publication combined with the absence of some metabolites in the saliva of dogs on day 30 of supplementation suggest that brown algae inhibit or turn off some pathways that could enhance plaque or calculus development. The exact mechanism of A. nodosum is still unclear and warrants further study.

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“…Since then, a systematic increase in interest in this field has been observed, which translates into the number of articles published (Figure 1). Thanks to the development of technology, new methodological and technical solutions, lipidomics is becoming an attractive branch of metabolomics (Figure 2) [6], which allows learning about cell lipid conditions. Linking the lipid composition with changes in the body enables the identification of metabolic pathways.…”

Section: Introductionmentioning

confidence: 99%

Lipidomic profiles as a tool to search for new biomarkers

Pisarska¹,

Wąsowicz²,

Gromadzińska³

2022

Int J Occup Med Environ Health

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Lipidomics belongs to the family of the so-called omics domains, which, based on modern chemical technologies, strive to explain the biological principles of the organism's functioning. Main biological functions of lipids include energy storage, the formation of cell membranes, and participation in the transmission of biological signals, and their dysregulation is responsible for the development of pathological states. Thanks to lipid profiling, potential biomarkers for disease diagnosis and prognosis can be identified. This paper discusses selected examples of the use of lipidomic tests in the diagnosis of the kidney, metabolic and neoplastic diseases based on research papers published over the last few years (since 2016). Only works based on the study of human biological material by mass spectrometry methods were taken into account. The examples of lipidomics application presented in this publication are only a few of the possibilities of this technique. As potential possibilities have already been discovered, the next step for the research community is to work on standardization of the approach to lipidomic research and to develop bioinformatics methods that allow efficient processing and analysis of large amounts of data generated in this technique. Int J Occup Med Environ Health. 2022;35(2)

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Analytical Strategies in Lipidomics for Discovery of Functional Biomarkers from Human Saliva

Cited by 23 publications

References 77 publications

Plasma Lipid Profiling Contributes to Untangle the Complexity of Moyamoya Arteriopathy

Plasma Lipid Profiling Contributes to Untangle the Complexity of Moyamoya Arteriopathy

Influence of Dietary Supplementation With a Powder Containing A.N. ProDen™ (Ascophyllum Nodosum) Algae on Dog Saliva Metabolome

Lipidomic profiles as a tool to search for new biomarkers

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