Analyzing the Effect of Strigolactones on the Motility Behavior of Rhizobia

Bernabéu-Roda, Lydia; López‐Ráez, Juan Antonio; Soto, Marı́a José

doi:10.1007/978-1-0716-1429-7_8

Cited by 2 publications

(2 citation statements)

References 31 publications

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“…Surface motility was analysed on semisolid MM plates containing 0.4% agarose and, when corresponds, supplemented with ethanol or a flavonoid (at final concentrations of 0.1% v / v and 1 μg/mL, respectively). Plates were prepared according to a rigorous protocol [ 47 ] since the water content of the medium is a crucial factor for surface motility. Each plate contained 20 mL of medium that was poured when the agar was relatively cool (∼50 °C).…”

Section: Methodsmentioning

confidence: 99%

“…Swarming is the fastest known type of bacterial motility on surfaces, and it is characterized by the coordinated multicellular migration of cells. This type of motility has been described in several rhizobial species [29,33,[42][43][44][45][46][47]. In contrast to swarming, surfing and sliding have only been described in the alfalfa symbiont Sinorhizobium meliloti [33,45,48].…”

Section: Introductionmentioning

confidence: 99%

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Surface Motility Regulation of Sinorhizobium fredii HH103 by Plant Flavonoids and the NodD1, TtsI, NolR, and MucR1 Symbiotic Bacterial Regulators

Alias‐Villegas

Fuentes-Romero

Cuéllar

et al. 2022

IJMS

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Bacteria can spread on surfaces to colonize new environments and access more resources. Rhizobia, a group of α- and β-Proteobacteria, establish nitrogen-fixing symbioses with legumes that rely on a complex signal interchange between the partners. Flavonoids exuded by plant roots and the bacterial transcriptional activator NodD control the transcription of different rhizobial genes (the so-called nod regulon) and, together with additional bacterial regulatory proteins (such as TtsI, MucR or NolR), influence the production of different rhizobial molecular signals. In Sinorhizobium fredii HH103, flavonoids and NodD have a negative effect on exopolysaccharide production and biofilm production. Since biofilm formation and motility are often inversely regulated, we have analysed whether flavonoids may influence the translocation of S. fredii HH103 on surfaces. We show that the presence of nod gene-inducing flavonoids does not affect swimming but promotes a mode of surface translocation, which involves both flagella-dependent and -independent mechanisms. This surface motility is regulated in a flavonoid-NodD1-TtsI-dependent manner, relies on the assembly of the symbiotic type 3 secretion system (T3SS), and involves the participation of additional modulators of the nod regulon (NolR and MucR1). To our knowledge, this is the first evidence indicating the participation of T3SS in surface motility in a plant-interacting bacterium. Interestingly, flavonoids acting as nod-gene inducers also participate in the inverse regulation of surface motility and biofilm formation, which could contribute to a more efficient plant colonisation.

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Section: Methodsmentioning

confidence: 99%