Anaphylatoxin, Histamine Depletion and Skin Reactions in the Rat

The Anaphylatoxin-Forming Capacity of Rat Serum as Influenced by Proteus Polysaccharides. Further Pharmacodynamic Differentiation of Anti-Allergic Polysaccharides and Steroids

“…A somewhat similar conclusion was reached by R o c h a e S il v a and R o t h s c h il d (12) who injected starch, agar or glycogen in doses some 10,000 times higher than those with which PS were found to achieve comparable effects in the present investigation.…”

On the Paw Oedema Resulting from Subcutaneous Injection of Histamine Liberators in the Rat

“…In any case, the data obtained in this study do not point to other mediators as being causally involved to an appreciable degree in this type of paw swelling. This holds particularly true for bradykinin which Rocha e Silva and his co-workers believe to be important in certain inflammatory reactions induced in the rat's paw [31,32,33]. Some such causative role of kinins has been denied for certain paw oedemas, particu larly for that elicited by mild heating by Urbanitz et al [42], Finally, a point involving technical details of the methods em ployed deserves some consideration.…”

Release of Bradykinin and the Mechanism of Production of a “Thermic Edema (45°C)” in the Rat’s Paw

“…Formation of an edema by such mild treatment as keeping the paw of an anesthetized rat at 45°C for half an hour, is worth studying for its possibility of throwing light upon the me- chanism of formation of other kinds of edema, such as the ana phylactoid edema produced by injection of dextran and ovo mucoid, or the so-called passive cutaneous anaphylaxis (PCA). Though in the two first mentioned edemas, the release of hista mine might play a rôle (Schächter and Talesnik, 1952;Halpern et al, 1959) in the PCA reaction the participation of histamine could be partially or totally excluded, since in the rat, after de pletion of the skin of its histamine content by previous treatment with 48/80, or submitting the animals to a high dosage of antihistaminics, the PCA reaction can be produced with equal inten sity (Rocha e Silva and Rothschild, 1955;Brocklehurst et al, 1955\ Inderbitzin andDobric, 1959;Ilalpem et al, 1959). But even for the production of the dextran and egg white edema the parti cipation of histamine as the most important mediator was ques tioned (Kramer, 1956).…”