Anatomic location of Mycoplasma mycoides subsp. capri and Mycoplasma agalactiae in naturally infected goat male auricular carriers

Gómez-Martín, Ángel; Fe, Christian de la; Amores, J.; Sánchez, Antonio; Contreras, A.; Paterna, A.; Buendía, A. J.; Corrales, J.C.

doi:10.1016/j.vetmic.2012.01.004

Cited by 31 publications

(33 citation statements)

References 36 publications

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“…Previously, we had studied the colonization of M. agalactiae wild-type strain PG2 and PLMs (Vpma phase-locked mutants) using this model (27). Besides the colonization and multiplication of M. agalactiae in the udder and simultaneous shedding in milk, it also leads to clinical signs and systemic spreading to various body sites (10,27,55,56). The latter would be an important added advantage for an infection model while performing a challenge infection experiment with single selected mutants to verify their attenuation.…”

Section: Discussionmentioning

confidence: 99%

Simultaneous Identification of Potential Pathogenicity Factors of Mycoplasma agalactiae in the Natural Ovine Host by Negative Selection

Hegde

Zimmermann

et al. 2015

Infect Immun

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Mycoplasmas possess complex pathogenicity determinants that are largely unknown at the molecular level. Mycoplasma agalactiae serves as a useful model to study the molecular basis of mycoplasma pathogenicity. The generation and in vivo screening of a transposon mutant library of M. agalactiae were employed to unravel its host colonization factors. Tn4001mod mutants were sequenced using a novel sequencing method, and functionally heterogeneous pools containing 15 to 19 selected mutants were screened simultaneously through two successive cycles of sheep intramammary infections. A PCR-based negative selection method was employed to identify mutants that failed to colonize the udders and draining lymph nodes in the animals. A total of 14 different mutants found to be absent from >95% of samples were identified and subsequently verified via a second round of stringent confirmatory screening where 100% absence was considered attenuation. Using this criterion, seven mutants with insertions in genes MAG1050, MAG2540, MAG3390, uhpT, eutD, adhT, and MAG4460 were not recovered from any of the infected animals. Among the attenuated mutants, many contain disruptions in hypothetical genes, implying their previously unknown role in M. agalactiae pathogenicity. These data indicate the putative role of functionally different genes, including hypothetical ones, in the pathogenesis of M. agalactiae. Defining the precise functions of the identified genes is anticipated to increase our understanding of M. agalactiae infections and to develop successful intervention strategies against it.

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Section: Discussionmentioning

confidence: 99%

Simultaneous Identification of Potential Pathogenicity Factors of Mycoplasma agalactiae in the Natural Ovine Host by Negative Selection

Hegde

Zimmermann

et al. 2015

Infect Immun

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“…In brief, 50 mL of inoculated or control DS, ejaculate, or extender were plated onto agar medium for mycoplasmas [9] in microtiter plates and the plates incubated for 48 hours at 37 C in a humidified atmosphere enriched with 5% CO 2 . Mycoplasma viability was always determined in duplicate.…”

Section: Mycoplasma Viabilitymentioning

confidence: 99%

“…Solid and liquid mycoplasma media [9] were used to test the presence of mycoplasmas. Liquid media were inoculated with 200 mL of each sample and incubated at 37 C in a 5% CO 2 humid atmosphere for 48 hours.…”

Section: Mycoplasma Culture and Pcrmentioning

confidence: 99%

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Survival capacity of Mycoplasma agalactiae and Mycoplasma mycoides subsp capri in the diluted semen of goat bucks and their effects on sperm quality

Gómez-Martín

Vieira

et al. 2015

Theriogenology

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“…máticos que presentan infecciones sistémicas crónicas (Gómez-Martín et al, 2012a). Estos individuos portadores, que a menudo resultan ser seronegativos, pueden estar infectados con cepas de micoplasmas con suficiente capacidad patógena para originar brotes de la enfermedad (Tardy et al, 2011).…”

Section: Introductionunclassified

Evaluación de la viabilidad y motilidad espermática en dosis seminales caprinas para el desarrollo de un modelo de contaminación experimental con Mycoplasmaspp

Gómez-Martín¹,

Vieira²,

Ondiz³

et al. 2012

Anales De Veterinaria De Murcia

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RESUMENEn los últimos años se ha detectado en diversos centros de inseminación artificial la presencia de machos caprinos en los que se han aislado Mycoplasma agalactiae y Mycoplasma mycoides subsp. capri en muestras de semen, sin que presenten ningún síntoma clínico asociado. La capacidad de las bacterias del género Mycoplasma spp. para producir efectos perjudiciales en la calidad espermática es un hecho constatado en diversas especies animales, incluido el hombre. Además, el riesgo de una posible transmisión venérea y afectación de la calidad espermática podría comprometer los programas de mejora genética caprinos fundamentados en la inseminación artificial. El objetivo del presente trabajo es desarrollar un modelo experimental para el estudio del efecto de la contaminación seminal con Mycoplasma spp. en ganado caprino. Para ello, evaluamos la viabilidad y motilidad espermática de dosis seminales, tras dos horas de incubación a 37 ºC en muestras previamente transportadas bajo dos condiciones de temperatura (4-5 °C y 15-16 ºC). Posteriormente se estudió el efecto de la adición del medio PPLO, en el que se preparan los inóculos de Mycoplasma spp., sobre la calidad espermática. El transporte de dosis seminales a 4 ºC ofrece mejores resultados de motilidad total en semen incubado a 37 ºC durante 120 minutos. El medio PPLO no ejerció efecto significativo sobre los porcentajes de espermatozoides vivos o mótiles totales a lo largo de 150 minutos de incubación. Con dicho medio, se pueden obtener valores de viabilidad y motilidad (total y progresiva) espermática superiores al 50% después de 150 y 60 minutos de I.S.S.N.: 0213-5434

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Anatomic location of Mycoplasma mycoides subsp. capri and Mycoplasma agalactiae in naturally infected goat male auricular carriers

Cited by 31 publications

References 36 publications

Simultaneous Identification of Potential Pathogenicity Factors of Mycoplasma agalactiae in the Natural Ovine Host by Negative Selection

Simultaneous Identification of Potential Pathogenicity Factors of Mycoplasma agalactiae in the Natural Ovine Host by Negative Selection

Survival capacity of Mycoplasma agalactiae and Mycoplasma mycoides subsp capri in the diluted semen of goat bucks and their effects on sperm quality

Evaluación de la viabilidad y motilidad espermática en dosis seminales caprinas para el desarrollo de un modelo de contaminación experimental con Mycoplasmaspp

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