Anatomical and Histological Comparison of the Alimentary Canal of &lt;I&gt;Dendroctonus micans&lt;/I&gt;, &lt;I&gt;D. ponderosae&lt;/I&gt;, &lt;I&gt;D. pseudotsugae pseudotsugae&lt;/I&gt;, &lt;I&gt;D. rufipennis&lt;/I&gt;, and &lt;I&gt;D. terebrans&lt;/I&gt; (Coleoptera: Scolytidae)

Díaz, Elba; Arciniega, Olga; Sánchez, Laura; Cisneros, Ramón; Zúñiga, Gerardo

doi:10.1603/0013-8746(2003)096[0144:aahcot]2.0.co;2

Cited by 28 publications

(39 citation statements)

References 9 publications

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“…Based on the detection of strictly anaerobic bacteria (i.e., Prevotella in this study and Clostridiales in other Dendroctonus species) [33], facultative anaerobes and strict aerobes in this study, we argue that other factors that may compromise the population density of certain bacteriaarethe compartmentalization of the gut [59, 60], its histolysis in the larval stage, the histogenesis in new adults, and the existence of a physicochemically heterogeneous environment (i.e., pH, protease and lysozyme production, O 2 gradients, redox potential), as demonstrated in other insect species [61–63]. …”

Section: Discussionmentioning

confidence: 93%

Structure and dynamics of the gut bacterial microbiota of the bark beetle, Dendroctonus rhizophagus (Curculionidae: Scolytinae) across their life stages

et al. 2017

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Bark beetles play an important role as agents of natural renovation and regeneration in coniferous forests. Several studies have documented the metabolic capacity of bacteria associated with the gut, body surface, and oral secretions of these insects; however, little is known about how the bacterial community structure changes during the life cycle of the beetles. This study represents the first comprehensive analysis of the bacterial community of the gut of the bark beetle D. rhizophagus during the insect’s life cycle using 454 pyrosequencing. A total of 4 bacterial phyla, 7 classes, 15 families and 23 genera were identified. The α-diversity was low, as demonstrated in previous studies. The dominant bacterial taxa belonged to the Enterobacteriaceae and Pseudomonadaceae families. This low α-diversity can be attributed to the presence of defensive chemical compounds in conifers or due to different morpho-physiological factors in the gut of these insects acting as strong selective factors. Members of the genera Rahnella, Serratia, Pseudomonas and Propionibacterium were found at all life stages, and the first three genera, particularly Rahnella, were predominant suggesting the presence of a core microbiome in the gut. Significant differences in β-diversity were observed, mainly due to bacterial taxa present at low frequencies and only in certain life stages. The predictive functional profiling indicated metabolic pathways related to metabolism of amino acids and carbohydrates, and membrane transport as the most significant in the community. These differences in the community structure might be due to several selective factors, such as gut compartmentalization, physicochemical conditions, and microbial interactions.

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Section: Discussionmentioning

confidence: 93%

Structure and dynamics of the gut bacterial microbiota of the bark beetle, Dendroctonus rhizophagus (Curculionidae: Scolytinae) across their life stages

et al. 2017

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“…In the acquired oblique coronal slices through the beetle's body, the different body segments such as the head including mouthparts, legs and wings and their respective muscles and intestinal (Baker and Estrin, 1974;Díaz et al, 2003) and genital organs (Calder, 1990) have been identified (Fig.1, see also supplementary material Movie1). Susceptibility artifacts were greatly reduced by using Shigemi tubes.…”

Section: Results and Discussion Anatomymentioning

confidence: 99%

Non-invasive lipid measurement in living insects using NMR microscopy

Schilling

Dworschak

Schopf

et al. 2012

Journal of Experimental Biology

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SUMMARYNuclear magnetic resonance (NMR) microscopy allows us to image and quantify the distribution of NMR-active nuclei in living specimens. Using high-field NMR microscopy at a magnetic field strength of 14.1T and strong gradients up to 3Tm -1 , we show that separation of fat and water nuclear resonances in living insects can be achieved. In contrast to destructive conventional photometric and mass measurements, we demonstrate exemplarily in the European spruce bark beetle that NMR can be efficiently used to quantify absolute fat and water content in living insects. Additionally, anatomic images with a spatial in-plane resolution up to 10m and with high soft tissue contrast were acquired. We demonstrate that fat distribution and fat consumption of living insects can be obtained by magnetic resonance imaging (MRI). This enables future research to address questions where single individuals have to be measured several times, which is not possible with conventional destructive methods. Supplementary material available online at

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“…As during regeneration, the regenerative cell groups fulfill the role of stem cells of the midgut epithelium (midgut primordial cells) during midgut epithelium formation in the first larval stage. In many insect species, regenerative cells are responsible for regeneration of the entire epithelium or only of its single cells (Reinhard et al, 1972;Hecker, 1977;Humbert, 1979;Dow, 1986;Sadrud-Din et al, 1996;Diaz et al, 1998Diaz et al, , 2000Diaz et al, , 2003Hakim et al, 1988Hakim et al, , 2001Cruz-Landim, 1999;Evangelista and Leite, 2003;Rost, 2006a,b). They are able to undergo mitotic divisions and to differentiate into a definite cell type, in this case midgut epithelial cells (Hakim et al, 2001).…”

Section: Discussionmentioning

confidence: 99%

“…These cells appear singly or in groups (or crypts) (Chapman, 1985;Hakim et al, 1988;Klag et al, 2002;Rost et al, 2005). In some insects accessory goblet cells are also midgut constituents (Kobayashi and Ando, 1983;Chapman, 1985;Uwo et al, 2002;Diaz et al, 2003).In most insect species, the midgut epithelium is formed during embryogenesis. However, in some species its development proceeds only during larval stages, whereas the remaining organs are being completely formed during embryogenesis (Ando, 1962;Schwalm, 1988;Rost et al, 2005).…”

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confidence: 99%

Ultrastructural studies of midgut epithelium formation in Lepisma saccharina L. (Insecta, Zygentoma)

Rost‐Roszkowska¹,

Piłka²,

Szymska³

et al. 2007

Journal of Morphology

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At the end of embryogenesis of Lepisma saccharina L. (Insecta, Zygentoma), when the stomodaeum and proctodaeum are completely formed, the midgut epithelium is replaced by the primary midgut, a yolk mass is surrounded by a cell membrane. Midgut epithelium formation begins in the 1st larval stage. Energids migrate toward the yolk periphery and aggregate just beneath the cell membrane. They are gradually enclosed by cell membrane folds of the primary midgut. Single cells are formed. Succeeding energids join just formed cells. Thus, groups of cells, regenerative cell groups, are formed. Their number gradually increases. The external cells of the regenerative cell groups transform into epithelial cells and their basal regions spread toward the next regenerative cell groups. Epithelial cells of neighboring regenerative cell groups join each other to form the epithelium. At the end of the 2nd larval stage, just before molting, degeneration of newly the formed epithelium begins. Remains of organelles and basal membrane occur between the regenerative cell groups. The new epithelium is formed from the regenerative cell groups, which are now termed stem cells of the midgut epithelium.

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Anatomical and Histological Comparison of the Alimentary Canal of <I>Dendroctonus micans</I>, <I>D. ponderosae</I>, <I>D. pseudotsugae pseudotsugae</I>, <I>D. rufipennis</I>, and <I>D. terebrans</I> (Coleoptera: Scolytidae)

Cited by 28 publications

References 9 publications

Structure and dynamics of the gut bacterial microbiota of the bark beetle, Dendroctonus rhizophagus (Curculionidae: Scolytinae) across their life stages

Structure and dynamics of the gut bacterial microbiota of the bark beetle, Dendroctonus rhizophagus (Curculionidae: Scolytinae) across their life stages

Non-invasive lipid measurement in living insects using NMR microscopy

Ultrastructural studies of midgut epithelium formation in Lepisma saccharina L. (Insecta, Zygentoma)

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