Anatomical, histochemical, and immunohistochemical observations on the gastrointestinal tract of Gallinula chloropus (Aves: Rallidae)

Abdellatif, Ahmed M.; Farag, A.S.; Metwally, Elsayed

doi:10.1186/s40850-022-00161-6

Cited by 4 publications

(2 citation statements)

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“…The whole organ was serially sectioned at 4‐μm thickness, and the obtained slides were labelled for subsequent analysis (Abdellatif et al., 2016). For the histological study, paraffin sections were dewaxed, rehydrated and stained with haematoxylin and eosin (H&E) and Masson's trichrome stains (Abdellatif et al., 2022; Suvarna et al., 2019).…”

Section: Methodsmentioning

confidence: 99%

Insights into microstructure and expression of markers of proliferation, apoptosis and T cells in the spleen of cattle egret (Bubulcus ibis)

Abdellatif,

Basha

2024

Anat Histol Embryol

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The spleen is the largest secondary lymphoid organ with significant roles in pathogen clearance. It is involved in several avian diseases. The cattle egret is a wild insectivorous bird of agricultural and socioeconomic importance. Data related to microstructural features of cattle egret spleen are lacking. The present study investigated the gross anatomical, histological and immunohistochemical characteristics of the cattle egret spleen. Proliferation (PCNA and PHH3), apoptosis (cleaved caspase 3, C.CASP3) and T‐cell (CD3 and CD8) markers were assessed. Grossly, the spleen appeared brownish red, oval‐shaped and located at the oesophago‐proventricular junction. Histologically, the spleen was surrounded by a thin capsule sending a number of trabeculae which contained branches of the splenic vessels. The white pulp consisted of the periarteriolar lymphoid sheath and periellipsoidal lymphatic sheath (PELS). The red pulp was formed of sinusoids and cords. The penicillar capillaries, which represent the terminal segments of the splenic arterial tree were highly branched, wrapped by prominent ellipsoids and directly connected to the splenic sinusoids, suggesting a closed type of circulation. Immunohistochemically, proliferating cell nuclear antigen (PCNA)‐expressing cells were distributed with high counts throughout the splenic parenchyma, being highest within the splenic cords and PELS. Both PHH3‐ and C.CASP3‐expressing cells revealed a similar pattern to that of PCNA, although with fewer counts. Large numbers of T cells were observed throughout the splenic parenchyma, mainly within the cords, as revealed by CD3 and CD8 immunoreaction. The present study provides a clear insight into the precise structure of the spleen in cattle egrets and thus improves our understanding about birds' immunity.

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Section: Methodsmentioning

confidence: 99%

Insights into microstructure and expression of markers of proliferation, apoptosis and T cells in the spleen of cattle egret (Bubulcus ibis)

Abdellatif,

Basha

2024

Anat Histol Embryol

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“…Bound antibodies were visualized by incubating the stained sections with diaminobenzidine solution (SK-4103, Vector Laboratories) for 2-10 minutes. All sections were finally counterstained with hematoxylin, dehydrated, and cleared (17,18).…”

Section: Immunohistochemistrymentioning

confidence: 99%

EXPRESSION OF INSULIN, GLUCAGON, SOMATOSTATIN, AND PANCREATIC POLYPEPTIDE IN THE PANCREAS OF THE EURASIAN MOORHEN (Gallinula chloropus)

Abdellatif

2023

SVR

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The pancreas is a complex gland that possesses both endocrine and exo-crine functions. The present study investigated the gross anatomy, histochemical fea-tures, and immunohistochemical expression of insulin, glucagon, somatostatin, and pancreatic polypeptide in the pancreas of the Eurasian moorhen. Grossly, the pancreas consisted of three duodenal lobes and unpaired splenic and gastric lobes. The pancre-atic capsule appeared thin with no distinct lobulation pattern. Three islet types were observed namely alpha, beta, and mixed types. The alpha-type islets formed mainly of glucagon- and somatostatin-expressing cells. The beta-type islets appeared relatively smaller than the two other islet types and formed predominately of insulin-expressing cells with a limited number of other endocrine cells. The mixed islets were formed by almost equal proportions of insulin-, glucagon- and somatostatin-expressing cells. A higher number of alpha-type islets was observed in the splenic lobe than in other pan-creatic lobes. Unlike other pancreatic endocrine cells which appeared oval or triangular in shape, somatostatin-expressing cells appeared with irregular outlines with cytoplas-mic processes contacting each other’s forming a meshwork within the islet. The results of this study revealed species-specific features of the endocrine and exocrine pancreas in the Eurasian moorhen and could suggest pancreatic functional differences depend-ing on feeding habits. Izražanje insulina, glukagona, somatostatina in pankreatičnega polipeptida v trebušni slinavki evrazijskega zelenonoge tukalice (Gallinula chloropus) Izvleček: Trebušna slinavka je kompleksna žleza z endokrino in eksokrino funkcijo. V tej študiji smo preučevali splošno anatomijo, histokemične značilnosti in imunohistokemično izražanje inzulina, glukagona, somatostatina in pankreas-nega polipeptida v trebušni slinavki evrazijske zelenonoge tukalice. Makroskopsko je bila trebušna slinavka sestavljena iz treh dvanajstnikovih režnjev ter neparnih vraničnega in želodčnega režnja. Kapsula trebušne slinavke je bila tanka, brez izrazitega vzorca lobulacije. Opazni so bili trije tipi otočkov - alfa, beta in mešani. Otočki tipa alfa so bili sestavljeni predvsem iz celic, ki so izražale glukagon in somatostatin. Otočki tipa beta so bili relativno manjši od drugih dveh tipov otočkov in sestavljeni pretežno iz celic, ki so izražale inzulin ter omejenega števila drugih endokrinih celic. Mešani tipi otočkov so bili sestavljeni iz enakega deleža celic, ki izražajo inzulin, glukagon in somatostatin. V vraničnem režnju je bilo opazno več otočkov tipa alfa kot v drugih režnjih trebušne slinavke. V primerjavi z drugimi endokrinimi celicami trebušne slinavke, ki so bile ovalne ali trikotne oblike, so bile celice, ki izražajo somatostatin, nepravilnih obrisov s citoplazems-kimi izrastki, ki so se stikali med seboj in tvorili mrežo znotraj otočka. Rezultati te študije so razkrili vrstno specifične značilnosti endokrine in eksokrine trebušne slinavke pri evrazijski zelenonogi tukalici in bi lahko nakazovali funkcionalne razlike v delovanju trebušne slinavke glede na prehranjevalne navade. Ključne besede: trebušna slinavka; otoček; inzulin; glukagon; somatostatin; polipeptid; sladkorna bolezen

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Descriptive embryological insights of the colorectum of quail embryos with concern to its functional morphology

Abdelhakeem,

Madkour

2024

BMC Vet Res

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Background Quail is an interesting emerging bird species gaining attention in developmental embryology research due to its small size, quick lifespan, and fast growth rate. These characteristics make quail an ideal model for examining the development of the gastrointestinal tract. Consequently, the embryonic development of the colorectum was conducted to provide a comprehensive understanding of its functions in digestion, absorption, and immunity. Methodology The morphological anatomy and microscopical structure of the colorectal wall of 74 embryos were studied using light and scanning electron microscopy (SEM). Histologically, the embryos were collected and dissected to extract the intestine. The samples were then fixed in 10% neutral buffer formalin for a minimum of 24 h, and in 2.5% glutaraldehyde buffer formalin for semithin processing and scanning electron microscopy. Results The wall of the embryonic colorectum on the hatching day consisted of three layers; mucosa, muscularis externa, and serosa. Mucosa was a simple layer of columnar enterocytes interspersed with goblet cells that appeared as cub-like shaped cells. Additionally, two ganglionic plexuses were also developed in the colorectal wall; Auerbach plexus (among the colorectal tunica muscularis) and Meissner plexus (submucosal plexus). Conclusion The morphological characteristics of the quail colorectum at different ages were closely related to its functional features.

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Anatomical, histochemical, and immunohistochemical observations on the gastrointestinal tract of Gallinula chloropus (Aves: Rallidae)

Cited by 4 publications

References 44 publications

Insights into microstructure and expression of markers of proliferation, apoptosis and T cells in the spleen of cattle egret (Bubulcus ibis)

Insights into microstructure and expression of markers of proliferation, apoptosis and T cells in the spleen of cattle egret (Bubulcus ibis)

EXPRESSION OF INSULIN, GLUCAGON, SOMATOSTATIN, AND PANCREATIC POLYPEPTIDE IN THE PANCREAS OF THE EURASIAN MOORHEN (Gallinula chloropus)

Descriptive embryological insights of the colorectum of quail embryos with concern to its functional morphology

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