“…Moreover, a substantial number of studies have actually reported improved heterologous expression of ancestral proteins as compared with their modern counterparts. Examples include: phosphate-binding protein 22 , periplasmic binding protein 23 , serum paraoxonase 24 , coagulation factor VIII 25 , titin 26 , haloalkane dehalogenases 27 , cytidine and adenine base editors 28 , diterpene cyclase 29 , rubisco 30 , endoglucanases 31 , L-amino acid oxidases 32 , laccases 33 , front-end D6-desaturases 34 and fatty acid photo-decarboxylases 35 . On a related note, recent studies on ancestral proteins have noted an improved capability to yield crystals suitable for X-ray structural determination 36,37 .…”