Anchorage-Independent Culture of Bovine Granulosa Cells: The Effects of Basic Fibroblast Growth Factor and Dibutyryl cAMP on Cell Division and Differentiation

Lavranos, Tina C.; Rodgers, Helen F.; Bertoncello, Ivan; Rodgers, Raymond J.

doi:10.1006/excr.1994.1084

Cited by 83 publications

(40 citation statements)

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“…Receptors for bFGF have been found on granulosa cells and oocytes (Shikone et al 1992, Wandji et al 1992, Ben-Haroush et al 2005. Basic FGF has been shown to stimulate proliferation of granulosa and ovarian stromal cells, and increase the mRNA expression of another stimulatory growth factor kit ligand (KITL; Lavranos et al 1994, Roberts & Ellis 1999, Nilsson et al 2001a, Nilsson & Skinner 2004. How bFGF interacts with AMH to regulate primordial to primary follicle transition is investigated in the current study.…”

Section: Introductionmentioning

confidence: 99%

Actions of anti-Müllerian hormone on the ovarian transcriptome to inhibit primordial to primary follicle transition

Nilsson¹,

Rogers²,

Skinner³

2007

Reproduction

171

102

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The oocytes found within the primordial follicles of mammalian ovaries remain quiescent for months to years until they receive the appropriate signals to undergo the primordial to primary follicle transition and initiate folliculogenesis. The molecular mechanisms and extracellular signaling factors that regulate this process remain to be fully elucidated. The current study investigates the mechanisms utilized by anti-Mü llerian hormone (AMH; i.e. Mü llerian inhibitory substance) to inhibit the primordial to primary follicle transition. Ovaries from 4-day-old rats were placed into organ culture and incubated in the absence or presence of AMH, either alone or in combination with known stimulators of follicle transition, including basic fibroblast growth factor (bFGF), kit ligand (KITL), or keratinocyte growth factor (KGF). Following 10 days of culture, the ovaries were sectioned, stained, and morphologically evaluated to determine the percentage of primordial versus developing follicles. As previously demonstrated, AMH treatment decreased primordial to primary follicle transition. Interestingly, AMH inhibited the stimulatory actions of KITL, bFGF, and KGF. Therefore, AMH can inhibit the basal and stimulated development of primordial follicles. To investigate the mechanism of AMH actions, the influence AMH has on the ovarian transcriptome was analyzed. AMH treatment when compared with controls was found to alter the expression of 707 genes. The overall effect of AMH exposure is to decrease the expression of stimulatory factors, increase the expression of inhibitory factors, and regulate cellular pathways (e.g. transforming growth factor b signaling pathway) that result in the inhibition of primordial follicle development. Analysis of the regulatory factors and cellular pathways altered by AMH provides a better understanding of the molecular control of primordial follicle development.

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Section: Introductionmentioning

confidence: 99%

Actions of anti-Müllerian hormone on the ovarian transcriptome to inhibit primordial to primary follicle transition

Nilsson¹,

Rogers²,

Skinner³

2007

Reproduction

171

102

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“…In mammals, this family consists of 18 secreted proteins (and four intracellular proteins called FGF homologous factors) that are grouped into subfamilies based on sequence homology (Itoh & Ornitz 2004). It has been recognized for over 20 years that FGF2 acts on granulosa cells to promote cell proliferation and decrease apoptosis and steroidogenesis (Gospodarowicz & Bialecki 1979, Baird & Hsueh 1986, Lavranos et al 1994, Vernon & Spicer 1994, and over the last decade several other FGFs have been implicated in ovarian function and follicle development, as recently reviewed (Chaves et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Mechanisms of fibroblast growth factor signaling in the ovarian follicle

Price

2015

Journal of Endocrinology

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Fibroblast growth factors (FGFs) have been shown to alter growth and differentiation of reproductive tissues in a variety of species. Within the female reproductive tract, the effects of FGFs have been focused on the ovary, and the most studied one is FGF2, which stimulates granulosa cell proliferation and decreases differentiation (decreased steroidogenesis). Other FGFs have also been implicated in ovarian function, and this review summarizes the effects of members of two subfamilies on ovarian function; the FGF7 subfamily that also contains FGF10, and the FGF8 subfamily that also contains FGF18. There are data to suggest that FGF8 and FGF18 have distinct actions on granulosa cells, despite their apparent similar receptor binding properties. Studies of non-reproductive developmental biology also indicate that FGF8 is distinct from FGF18, and that FGF7 is also distinct from FGF10 despite similar receptor binding properties. In this review, the potential mechanisms of differential action of FGF7/FGF10 and FGF8/FGF18 during organogenesis will be reviewed and placed in the context of follicle development. A model is proposed in which FGF8 and FGF18 differentially activate receptors depending on the properties of the extracellular matrix in the follicle.

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“…This results in an increased supply of nutrients and precursors, therefore supporting the growth of the dominant follicle in bovine ovaries (Berisha et al 2000a). In addition, FGF2 may play a significant role in bovine granulosa cell steroidogenesis (Vernon & Spicer 1994), anti-apoptotic action (Peluso et al 2001) and differentiation of granulosa cells (GCs) into luteal cells during the process of luteinisation (Lavranos et al 1994).…”

Section: Introductionmentioning

confidence: 99%

Changes in fibroblast growth factor 2 and its receptors in bovine follicles before and after GnRH application and after ovulation

Berisha

Šteffl²,

Amselgruber³

et al. 2006

Reproduction

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The aim of this study was to evaluate the expression pattern of fibroblast growth factor 2 (FGF2), its receptor variants (FGFR1IIIc, FGFR2IIIc) and nucleolin in time-defined follicle classes before and after GnRH application and after ovulation in the cow. Ovaries containing preovulatory follicles or new corpora lutea (CL) were collected at approximately 0, 4, 10, 20 and 25 h (follicles) and 60 h (new CL) relative to injection of GnRH to induce an LH surge (n 5 5 animals per group). The expressions of FGF2 and FGFR1IIIc mRNA were significantly up-regulated only in the follicle group 4 h after GnRH (during the LH surge) with a significant down-regulation immediately afterwards. Western blot analyses showed two protein bands with at 22 and 18 kDa with apparent up-regulation beginning with the LH surge (4 h) and maximum levels 20 h after GnRH. FGF2 protein in follicles collected at 0 h (before LH surge) was localised in theca tissue (endothelial and pericytes of blood vessels) but not in granulosa cells (GCs). The FGF2 staining (by immunohistochemistry) pattern changed dramatically after the LH surge for a short period (about 2 days) and FGF2 protein was localised dominantly in the nucleus of many GCs, while most capillary endothelial cells were FGF2 immunonegative. In conclusion, the novel observation of FGF2 up-regulation and the distinct change in FGF2 localisation from theca (cytoplasm of endothelial cells) to the nucleus of GCs after the LH surge may be important for survival of GCs or for the transition of the GCs to luteal cells.

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Anchorage-Independent Culture of Bovine Granulosa Cells: The Effects of Basic Fibroblast Growth Factor and Dibutyryl cAMP on Cell Division and Differentiation

Cited by 83 publications

References 0 publications

Actions of anti-Müllerian hormone on the ovarian transcriptome to inhibit primordial to primary follicle transition

Actions of anti-Müllerian hormone on the ovarian transcriptome to inhibit primordial to primary follicle transition

Mechanisms of fibroblast growth factor signaling in the ovarian follicle

Changes in fibroblast growth factor 2 and its receptors in bovine follicles before and after GnRH application and after ovulation

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