Anchoring plant metallothioneins to the inner face of the plasma membrane of Saccharomyces cerevisiae cells leads to heavy metal accumulation

Ruţă, Lavinia L.; Lin, Ya-Fen; Kissen, Ralph; Nicolau, Ioana; Neagoe, Aurora; Ghenea, Simona; Bones, Atle M.; Fărcăşanu, Ileana C.

doi:10.1371/journal.pone.0178393

Cited by 17 publications

(15 citation statements)

References 47 publications

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“…Gonzalez-Mendoza et al [246] examined Cd and Cu stress in Avicennia germinate plants, and they observed a direct relationship between the overexpression of AvPCS and AvMT2 genes and homeostasis and detoxification of Cd and Cu in these plant cells. Additionally, it was shown that the expression of MT genes in yeast strains screened form A. thaliana, which were exposed to several metal stress conditions, MT3 was the best candidate for metal phytoremediation [247]. Today, genetic engineering techniques can improve the effectiveness of phytoremediation in plants [248].…”

Section: Phytochelatins and Metallothionein For Cadmium Phytoremediationmentioning

confidence: 99%

Phytoremediation of Cadmium: Physiological, Biochemical, and Molecular Mechanisms

Raza

Habib

Najafi-Kakavand

et al. 2020

Biology

185

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Cadmium (Cd) is one of the most toxic metals in the environment, and has noxious effects on plant growth and production. Cd-accumulating plants showed reduced growth and productivity. Therefore, remediation of this non-essential and toxic pollutant is a prerequisite. Plant-based phytoremediation methodology is considered as one a secure, environmentally friendly, and cost-effective approach for toxic metal remediation. Phytoremediating plants transport and accumulate Cd inside their roots, shoots, leaves, and vacuoles. Phytoremediation of Cd-contaminated sites through hyperaccumulator plants proves a ground-breaking and profitable choice to combat the contaminants. Moreover, the efficiency of Cd phytoremediation and Cd bioavailability can be improved by using plant growth-promoting bacteria (PGPB). Emerging modern molecular technologies have augmented our insight into the metabolic processes involved in Cd tolerance in regular cultivated crops and hyperaccumulator plants. Plants’ development via genetic engineering tools, like enhanced metal uptake, metal transport, Cd accumulation, and the overall Cd tolerance, unlocks new directions for phytoremediation. In this review, we outline the physiological, biochemical, and molecular mechanisms involved in Cd phytoremediation. Further, a focus on the potential of omics and genetic engineering strategies has been documented for the efficient remediation of a Cd-contaminated environment.

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Section: Phytochelatins and Metallothionein For Cadmium Phytoremediationmentioning

confidence: 99%

Phytoremediation of Cadmium: Physiological, Biochemical, and Molecular Mechanisms

Raza

Habib

Najafi-Kakavand

et al. 2020

Biology

185

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“…The strains were obtained from EUROSCARF (European S. cerevisiae Archive for Functional Analysis, ) and were propagated, grown, and maintained in YPD medium (1% yeast extract, 2% polypeptone, 2% glucose) or SD (0.17% yeast nitrogen base without amino acids, 0.5% (NH 4 ) 2 SO 4 , 2% glucose, supplemented with the necessary amino acids) [18]. The strains transformed with the plasmids harboring MT cDNA-s [10] were selected and maintained on SD lacking uracil (SD-Ura). For induction of MT cDNA expression, cells were pre-grown in synthetic medium containing 2% raffinose (SRaf-Ura) before being shifted to galactose-containing media [19].…”

Section: Methodsmentioning

confidence: 99%

“…In this study, we investigated the possibility to obtain silver-accumulating cells by heterologous expression of plant MTs targeted to the inner face of the yeast plasma membrane. Differently from our previous study, in which recombinant plant MTs overlapped the innate MT (ScMT or Cup1) [10], in the present study the plant MTs were expressed in yeast cells not expressing their innate MT, Cup1.…”

Section: Introductionmentioning

confidence: 93%

“…The prerequisite for efficient bioremediation techniques is the development of organisms modified to accumulate the contaminant without developing the inherent toxicity symptoms. Recently, we found that Saccharomyces cerevisiae cells expressing plant metallothioneins (MTs) targeted to the cytosolic face of the plasma membrane accumulate divalent metal cations such as Cd(II), Co(II), Cu(II), Mn(II), or Ni(II) [10]. MTs are metal-binding proteins found across most taxonomic groups involved in the heavy metal tolerance of many eukaryotes, including yeasts, mammals, and plants [11].…”

Section: Introductionmentioning

confidence: 99%

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Accumulation of Ag(I) by Saccharomyces cerevisiae Cells Expressing Plant Metallothioneins

Ruţă

Banu

Neagoe

et al. 2018

Cells

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The various applications of Ag(I) generated the necessity to obtain Ag(I)-accumulating organisms for the removal of surplus Ag(I) from contaminated sites or for the concentration of Ag(I) from Ag(I)-poor environments. In this study we obtained Ag(I)-accumulating cells by expressing plant metallothioneins (MTs) in the model Saccharomyces cerevisiae. The cDNAs of seven Arabidopsis thaliana MTs (AtMT1a, AtMT1c, AtMT2a, AtMT2b, AtMT3, AtMT4a and AtMT4b) and four Noccaea caerulescens MTs (NcMT1, NcMT2a, NcMT2b and NcMT3) fused to myrGFP displaying an N-terminal myristoylation sequence for plasma membrane targeting were expressed in S. cerevisiae and checked for Ag(I)-related phenotype. The transgenic yeast cells were grown in copper-deficient media to ensure the expression of the plasma membrane high-affinity Cu(I) transporter Ctr1, and also to elude the copper-related inhibition of Ag(I) transport into the cell. All plant MTs expressed in S. cerevisiae conferred Ag(I) tolerance to the yeast cells. Among them, myrGFP-NcMT3 afforded Ag(I) accumulation under high concentration (10–50 μM), while myrGFP-AtMT1a conferred increased accumulation capacity under low (1 μM) or even trace Ag(I) (0.02–0.05 μM). The ability to tolerate high concentrations of Ag(I) coupled with accumulative characteristics and robust growth showed by some of the transgenic yeasts highlighted the potential of these strains for biotechnology applications.

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“…Furthermore, the overexpression of MT1 of the copper-accumulator plant Elsholtzia haichowensis in tobacco plants allowed copper accumulation in roots [31]. In this regard, the six MTs of A. thaliana and the four MTs of Noccacea caerulescens expressed and anchored to the inner face of the plasma membrane through a myristoil tail fused to GFP and fused to the C-terminal region of MTs allowed the accumulation of copper and other heavy metals in yeast [32]. Thus, copper excess normally induced the synthesis of MTs in plants allowing copper accumulation.…”

Section: Mechanisms Of Copper Accumulation In Plants and Marine Macromentioning

confidence: 99%

Mechanisms of Copper Tolerance, Accumulation, and Detoxification in the Marine Macroalga Ulva compressa (Chlorophyta): 20 Years of Research

Moenne

Gómez

Laporte

et al. 2020

Plants

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Copper induces an oxidative stress condition in the marine alga Ulva compressa that is due to the production of superoxide anions and hydrogen peroxide, mainly in organelles. The increase in hydrogen peroxide is accompanied by increases in intracellular calcium and nitric oxide, and there is a crosstalk among these signals. The increase in intracellular calcium activates signaling pathways involving Calmodulin-dependent Protein Kinases (CaMKs) and Calcium-Dependent Protein Kinases (CDPKs), leading to activation of gene expression of antioxidant enzymes and enzymes involved in ascorbate (ASC) and glutathione (GSH) synthesis. It was recently shown that copper also activates Mitogen-Activated Protein Kinases (MAPKs) that participate in the increase in the expression of antioxidant enzymes. The increase in gene expression leads to enhanced activities of antioxidant enzymes and to enhanced levels of ASC and GSH. In addition, copper induces an increase in photosynthesis leading to an increase in the leve of Nicotinamide Adenine Dinucleotide Phosphate (NADPH). Copper also induces an increase in activities of enzymes involved in C, N, and S assimilation, allowing the replacement of proteins damaged by oxidative stress. The accumulation of copper in acute exposure involved increases in GSH, phytochelatins (PCs), and metallothioneins (MTs) whereas the accumulation of copper in chronic exposure involved only MTs. Acute and chronic copper exposure induced the accumulation of copper-containing particles in chloroplasts. On the other hand, copper is extruded from the alga with an equimolar amount of GSH. Thus, the increases in activities of antioxidant enzymes, in ASC, GSH, and NADPH levels, and in C, N, and S assimilation, the accumulation of copper-containing particles in chloroplasts, and the extrusion of copper ions from the alga constitute essential mechanisms that participate in the buffering of copper-induced oxidative stress in U. compressa.

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Anchoring plant metallothioneins to the inner face of the plasma membrane of Saccharomyces cerevisiae cells leads to heavy metal accumulation

Cited by 17 publications

References 47 publications

Phytoremediation of Cadmium: Physiological, Biochemical, and Molecular Mechanisms

Phytoremediation of Cadmium: Physiological, Biochemical, and Molecular Mechanisms

Accumulation of Ag(I) by Saccharomyces cerevisiae Cells Expressing Plant Metallothioneins

Mechanisms of Copper Tolerance, Accumulation, and Detoxification in the Marine Macroalga Ulva compressa (Chlorophyta): 20 Years of Research

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