Ancient and pervasive expansion of adaptin-related vesicle coat machinery across Parabasalia

Maciejowski, William; Gile, Gillian H.; Jerlström-Hultqvist, Jon; Dacks, Joel B.

doi:10.1016/j.ijpara.2023.01.002

Cited by 5 publications

(5 citation statements)

References 89 publications

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“…T. tenax appears to have lost COMMD5, COMMD6 and COMMD8. T. gallinae is seen to be missing COMMD1, 2, 6, 7, 8, and 9 along with CCDC22 and DENND10 which could have been a result of its poorer BUSCO score (Maciejowski et al, 2023). Among Tritrichomonadea, T. foetus appears to have lost COMMD4 along with COMMD1, while containing single homologs of other CCC complex proteins except for an expansion of three genes of CCDC93.…”

Section: Resultsmentioning

confidence: 99%

“…Two distinct Parabasalia lineages; Trichomonadea and Tritrichomonadea have majorly adopted a parasitic lifestyle (Malik et al, 2011). Recently, free-living Anaeramoebae were shown to represent the closest sister lineage of Parabasalia and found to serve a good contrast of lifestyles between related organisms (Stairs et al, 2021), (Maciejowski et al, 2023).…”

Section: Introductionmentioning

confidence: 99%

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Tracing back the expansion of the endomembrane rescue systems in parasitic Parabasalids to the ancestral origin in its free-living sister lineage

Shinde,

Kucerova,

Dacks

et al. 2024

Preprint

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Early Endosomes sort transmembrane cargo whether for lysosomal degradation or retrieval to the plasma membrane or the Golgi complex. Endosomal retrieval in eukaryotes is governed by the anciently homologous Retromer or Retriever complexes. Each comprises a core tri-protein subcomplex, membrane-deformation proteins, and interacting partner complexes, together retrieving a variety of known cargo proteins. Trichomonas vaginalis; a sexually transmitted human parasite uses the endomembrane system for pathogenesis. It has massively and selectively expanded its endomembrane protein complement, the evolutionary path of which has been largely unexplored. Our molecular evolutionary study of Retromer, Retriever and associated machinery in parabasalids and its free-living sister lineage of Anaeramoeba, demonstrates specific expansion of the Retromer machinery, contrasting with the Retriever components. We also observe partial loss of Commander complex and Sorting Nexins in Parabasalia but complete retention in Anaeramoeba. Notably, we identify putative parabasalid Sorting Nexin analogues. Finally, we report the first Retriever protein localization in a non- metazoan group along with Retromer protein localization in T. vaginalis. Therefore, we provide a unique genome expansion study of endomembrane trafficking system in parasitic and free- living protists alike.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Tracing back the expansion of the endomembrane rescue systems in parasitic Parabasalids to the ancestral origin in its free-living sister lineage

Shinde,

Kucerova,

Dacks

et al. 2024

Preprint

Self Cite

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“…However, LDLR was seen to be completely absent in A. ignava but, alternatively, five genes were found in Anaeramoeba flamelloides of the Anaeramoeba lineage. This could have been because of the fragmented datasets of A. ignava ( Maciejowski et al, 2023 ). α 5 β 1 -integrin proteins were seen to be completely missing in both parabasalids and Anaeramoeba .…”

Section: Resultsmentioning

confidence: 99%

“…T. tenax appears to have lost COMMD5, COMMD6 and COMMD8. T. gallinae was seen to be missing COMMD1, COMMD2, COMMD6, COMMD7, COMMD8 and COMMD9, along with CCDC22 and DENND10, which could have been a result of its poorer BUSCO score ( Maciejowski et al, 2023 ). Among Tritrichomonadea, T. foetus appears to have lost COMMD4 along with COMMD1, while containing single homologs of other CCC complex proteins except for an expansion of three genes for CCDC93.…”

Section: Resultsmentioning

confidence: 99%

“…Two distinct Parabasalia lineages, Trichomonadea and Tritrichomonadea, have primarily adopted a parasitic lifestyle ( Malik et al, 2011 ). Recently, free-living Anaeramoebae were shown to represent the closest sister lineage of Parabasalia and found to serve a good contrast of lifestyles among related organisms ( Stairs et al, 2021 ; Maciejowski et al, 2023 ).…”

Section: Introductionmentioning

confidence: 99%

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The retromer and retriever systems are conserved and differentially expanded in parabasalids

Shinde,

Kučerová,

Dacks

et al. 2024

Journal of Cell Science

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Early Endosomes sort transmembrane cargo whether for lysosomal degradation or retrieval to the plasma membrane or the Golgi complex. Endosomal retrieval in eukaryotes is governed by the anciently homologous Retromer or Retriever complexes. Each comprises a core tri-protein subcomplex, membrane-deformation proteins, and interacting partner complexes, together retrieving a variety of known cargo proteins. Trichomonas vaginalis; a sexually transmitted human parasite uses the endomembrane system for pathogenesis. It has massively and selectively expanded its endomembrane protein complement, the evolutionary path of which has been largely unexplored. Our molecular evolutionary study of Retromer, Retriever and associated machinery in parabasalids and its free-living sister lineage of Anaeramoeba, demonstrates specific expansion of the Retromer machinery, contrasting with the Retriever components. We also observe partial loss of Commander complex and Sorting Nexins in Parabasalia but complete retention in Anaeramoeba. Notably, we identify putative parabasalid Sorting Nexin analogues. Finally, we report the first Retriever protein localization in a non-metazoan group along with Retromer protein localization in T. vaginalis.

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A unique symbiosome in an anaerobic single-celled eukaryote

Jerlström-Hultqvist,

Gallot-Lavallée,

Salas-Leiva

et al. 2024

Nat Commun

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Symbiotic relationships between eukaryotes and prokaryotes played pivotal roles in the evolution of life and drove the emergence of specialized symbiotic structures in animals, plants and fungi. The host-evolved symbiotic structures of microbial eukaryotes – the vast majority of such hosts in nature – remain largely unstudied. Here we describe highly structured symbiosomes within three free-living anaerobic protists (Anaeramoeba spp.). We dissect this symbiosis using complete genome sequencing and transcriptomics of host and symbiont cells coupled with fluorescence in situ hybridization, and 3D reconstruction using focused-ion-beam scanning electron microscopy. The emergence of the symbiosome is underpinned by expansion of gene families encoding regulators of membrane trafficking and phagosomal maturation and extensive bacteria-to-eukaryote lateral transfer. The symbionts reside deep within a symbiosomal membrane network that enables metabolic syntrophy by precisely positioning sulfate-reducing bacteria alongside host hydrogenosomes. Importantly, the symbionts maintain connections to the Anaeramoeba plasma membrane, blurring traditional boundaries between ecto- and endosymbiosis.

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Ancient and pervasive expansion of adaptin-related vesicle coat machinery across Parabasalia

Cited by 5 publications

References 89 publications

Tracing back the expansion of the endomembrane rescue systems in parasitic Parabasalids to the ancestral origin in its free-living sister lineage

Tracing back the expansion of the endomembrane rescue systems in parasitic Parabasalids to the ancestral origin in its free-living sister lineage

The retromer and retriever systems are conserved and differentially expanded in parabasalids

A unique symbiosome in an anaerobic single-celled eukaryote

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