Androgen-dependent expression of cystatin-related protein (CRP) in the exorbital lacrimal gland of the rat

Winderickx, Joris; Vercaeren, Inge; Verhoeven, Guido; Heyns, Walter

doi:10.1016/0960-0760(94)90141-4

Cited by 31 publications

(25 citation statements)

References 16 publications

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“…8, the pattern of induction in adult female rats was quite similar to that observed in young animals. During secondary induction, on the other hand, the induction proceeded much more rapidly, confirming previous biochemical results (Winderickx et al 1994), and also in a more homogeneous manner over all epithelial cells in the section.…”

Section: Induction Of Crp and C3 Expression By Exogenous Androgenssupporting

confidence: 87%

“…Two of these genes, encoding the slightly different proteins CRP-1 and CRP-2 (Winderickx et al 1990), have been cloned and sequenced (Devos et al 1993). Both of these genes are expressed and androgen-regulated in the ventral prostate, but only the CRP-1 gene is expressed and androgen-regulated in the lacrimal gland (Winderickx et al 1990(Winderickx et al , 1994. This functional homology between the prostate and lacrimal gland could be extended to the expression of PBP-C3 (Aumüller et al 1995;.…”

Section: Introductionmentioning

confidence: 96%

“…Furthermore, the androgen regulation of CRP and C3 can also be studied in the lacrimal glands of female rats (Winderickx et al 1994;Vercaeren et al 1996) and in primary cultures of lacrimal acinar cells .…”

Section: Introductionmentioning

confidence: 99%

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Expression of cystatin-related protein and of the C3-component of prostatic-binding protein during postnatal development in the rat ventral prostate and lacrimal gland

Vercaeren

Vanaken²,

Dorpe

et al. 1998

Cell and Tissue Research

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The expression of cystatin-related protein (CRP) and of the C3-component of prostatic-binding protein (PBP) during postnatal development of the rat was studied by Northern blotting, dot blot and in situ hybridisation, and by radioimmunoassay or immunoblotting. In intact male rats, very little or no PBP-C3 could be detected in the prostate at 10 days, but at 20 days there was already strong expression. By in situ hybridisation, the first expression of C3 mRNA was observed at 13 days in the prostate and at 22 days in the lacrimal gland. For CRP, this occurred at 16 and 22 days, respectively. Neither CRP nor C3 was expressed in prepubertal male rats castrated at day 1 or day 10 or in female rats. Androgen treatment of intact male animals did not advance the expression of both mRNAs in the prostate, but did so in the lacrimal gland with first expression of C3 at 19 instead of 22 days and of CRP at 13 instead of 22 days. Identical values were obtained in female rats. Androgen treatment of castrated adult male rats resulted in a more rapid and homogeneous secondary induction. Positive immunostaining for the androgen receptor (AR) was observed in the lacrimal gland at 7 days, but its concentration, estimated by immunoblotting, was still low at 10 days. Maximal levels, reached at 30 days, were markedly higher in male than in female rats. In conclusion, CRP and C3 are induced by androgens in prepubertal rats. The time point of induction, however, is probably determined by other tissue and differentiation-dependent factors in addition to androgens and the AR.

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Section: Induction Of Crp and C3 Expression By Exogenous Androgenssupporting

confidence: 87%

Section: Introductionmentioning

confidence: 96%

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Expression of cystatin-related protein and of the C3-component of prostatic-binding protein during postnatal development in the rat ventral prostate and lacrimal gland

Vercaeren

Vanaken²,

Dorpe

et al. 1998

Cell and Tissue Research

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“…Genes that are up-regulated by androgen in the prostate include prostate-specific antigen (PSA) (15,16), prostatein C3 (17), sex-limited protein (slp) (18), spermine-binding protein (19,20), prostate-binding protein C2A (21), cystatin-related protein 2 (22,23), calreticulin (24), and probasin (25,26). Androgen ablation-induced genes include clusterin͞sulfate glycoprotein-2 (SGP-2)͞testosterone repressed prostate mRNA-2 (TRPM-2) (27,28), transforming growth factor ␤ (29), rat ventral prostate gene 1 (RVP.1) (30), glutathione S-transferase (31), c-myc (32), c-fos (33), and matrix carboxyglutamic acid protein (30).…”

mentioning

confidence: 99%

Genes regulated by androgen in the rat ventral prostate

Wang

Tufts

Haleem

et al. 1997

Proc. Natl. Acad. Sci. U.S.A.

106

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Genes that are regulated by androgen in the prostate were studied in the rat. Four of the less than 10 genes that are down-regulated by androgen in the ventral prostate of a 7-day castrated rat were identified; their mRNAs decayed with identical kinetics. Twenty-five of the estimated 56 genes that are up-regulated by androgen in the castrated prostate have been isolated. The up-regulated genes fall into two kinetic types. Early genes are significantly up-regulated by 6.5 hr whereas the delayed genes respond mainly after 24 hr from the time of androgen replacement. These androgen-response genes are also regulated in the prostate by castration, indicating that these genes could play important roles in androgen-induced regrowth and͞or castration-induced regression of the prostate during hormonal manipulation. A survey of the tissue specificity showed that the androgen-response gene expression program in the prostate is mainly prostatespecific. Total RNA Northern blot analysis detects the expression of about 16 up-regulated genes and 3 down-regulated genes in the prostate only. Four up-regulated genes and one down-regulated gene are regulated by androgen in both the prostate and seminal vesicles but not in other organs. The expression of the remaining androgen-response genes is not limited to the prostate but is only responsive to androgen in the prostate. This survey of the androgen-response gene expression program provides insights into the molecular and cellular mechanisms of androgen action in the prostate.

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“…The 20-kDa protein gene codes for a major androgen-regulated secretory protein in rat ventral prostate and lacrimal gland (18,19,26,27). Studies in our laboratory have shown that within the first intron of this gene is a complex enhancer that responds selectively to AR (Fig.…”

mentioning

confidence: 99%

Androgen-dependent Protein Interactions within an Intron 1 Regulatory Region of the 20-kDa Protein Gene

Avellar

Gregory

Power

et al. 1997

Journal of Biological Chemistry

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The 20-kDa protein gene is androgen regulated in rat ventral prostate. Intron 1 contains a 130-base pair complex response element (D2) that binds androgen (AR) and glucocorticoid receptor (GR) but transactivates only with AR in transient cotransfection assays in CV1 cells using the reporter vector D2-tkCAT. To better understand the function of this androgen-responsive unit, nuclear protein interactions with D2 were analyzed by DNase I footprinting in ventral prostate nuclei of intact or castrated rats and in vitro with ventral prostate nuclear protein extracts from intact, castrated, and testosterone-treated castrated rats. Multiple androgendependent protected regions and hypersensitive sites were identified in the D2 region with both methods. Mobility shift assays with 32 P-labeled oligonucleotides spanning D2 revealed specific interactions with ventral prostate nuclear proteins. Four of the D2-protein complexes decreased in intensity within 24 h of castration. UV cross-linking of the androgen-dependent DNA binding proteins identified protein complexes of approximately140and55kDa.Theresultsdemonstrateandrogendependent nuclear protein-DNA interactions within the complex androgen response element D2.

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Androgen-dependent expression of cystatin-related protein (CRP) in the exorbital lacrimal gland of the rat

Cited by 31 publications

References 16 publications

Expression of cystatin-related protein and of the C3-component of prostatic-binding protein during postnatal development in the rat ventral prostate and lacrimal gland

Expression of cystatin-related protein and of the C3-component of prostatic-binding protein during postnatal development in the rat ventral prostate and lacrimal gland

Genes regulated by androgen in the rat ventral prostate

Androgen-dependent Protein Interactions within an Intron 1 Regulatory Region of the 20-kDa Protein Gene

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