Androgen, estrogen and progesterone receptor expression in the human uterus during the menstrual cycle

Mertens, H; Heineman, Maas Jan; Theunissen, P. H. M. H.; Jong, Frank H. de; Evers, Johannes L.H.

doi:10.1016/s0301-2115(00)00554-6

Cited by 113 publications

(77 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In normal cycling rodents (Katsuda et al 1999, Pelletier et al 2000, Wang et al 2000, Andersson et al 2001, Mendoza-Rodríguez et al 2003 and humans (Mertens et al 2001, Punyadeera et al 2003, ER is the dominant subtype expressed in the uterus, being localized to the epithelium, stroma, myometrium and some blood vessels. By comparison, ER is more weakly expressed.…”

Section: Introductionmentioning

confidence: 99%

Analysis of spatial and temporal expression patterns of bone morphogenetic protein family members in the rat uterus over the estrous cycle

Erickson

Fuqua²,

Shimasaki³

2004

Journal of Endocrinology

123

191

View full text Add to dashboard Cite

Recent studies have demonstrated that bone morphogenetic proteins (BMPs) play fundamental roles in female fertility. This is particularly evident in terms of the ovary. One major question that is just beginning to be addressed is the role of BMPs in the non-pregnant uterus. To help fill this gap, we used in situ hybridization to investigate the expression of BMP family members in the rat uterus over the estrous cycle. We found that the endometrial/uterine cycle is accompanied by the expression of several components of the BMP pathway -including ligands, receptors and antagonists. The mRNAs encoding BMP receptors are expressed in the epithelial (BMP-RIA, -RIB and -RII), periluminal stroma (BMP-RIA and -RII) and smooth muscle cells (BMP-RIA and -RII). The expression of all three receptors showed clear cyclic variations. The mRNAs encoding BMP ligands were highly expressed in the periluminal stroma (BMP-2 and -7) and glandular epithelium (BMP-7). The expression of BMP-2, but not BMP-7, was cyclical. Notably, the periluminal stroma expressed noggin mRNA. In the blood vascular system, BMP-4, -6 and -RII mRNAs were expressed in myometrial endothelial cells. Interestingly, follistatin, noggin, and BMP-4, -6 and -7 mRNAs were expressed in eosinophilic leukocytes, suggesting unexpected roles for eosinophil-derived BMPs in uterine function. We conclude that BMP ligands, receptors and antagonists are expressed in spatially and temporally restricted patterns that are consistent with a physiological role for these regulatory molecules in promoting uterine cellular processes including cell proliferation, differentiation and apoptosis during the cycle.

show abstract

Section: Introductionmentioning

confidence: 99%

Analysis of spatial and temporal expression patterns of bone morphogenetic protein family members in the rat uterus over the estrous cycle

Erickson

Fuqua²,

Shimasaki³

2004

Journal of Endocrinology

123

191

View full text Add to dashboard Cite

show abstract

“…Immunohistochemical localization of AR in the human endometrium has been reported (Mertens et al 2001, Slayden et al 2001, Apparao et al 2002. Positive immunostaining of AR has been described in endometrial stroma and epithelium in normo-ovulatory women and decreases steadily from the early proliferative phase to the mid-secretory phase (Mertens et al 2001).…”

Section: Introductionmentioning

confidence: 99%

Testosterone inhibits matrix metalloproteinase-1 production in human endometrial stromal cells in vitro

Ishikawa¹,

Harada²,

Kubota³

et al. 2007

Reproduction

View full text Add to dashboard Cite

Androgen receptor (AR) is reported to be expressed in human uterine endometrium, but not much information is available on the role of androgens in human endometrium. The purpose of this study is to investigate the role of androgens in the regulation of matrix metalloproteinase (MMP)-1, which is one of the important MMPs for menstruation and embryo implantation in human endometrium. Human endometrial stromal cells (HESCs) were obtained from human endometrium by enzymatic dissociation method. Purified HESCs were incubated with 17b-estradiol (E2), testosterone, or E2Ctestosterone. Progestins (natural progesterone or medroxyprogesterone acetate) or vehicle (dimethyl sulfoxide) were also added to the media instead of testosterone. Furthermore, hydroxyflutamide (FLU), a specific AR antagonist, was also supplemented to cultured media. The amounts of MMP-1 in cultured media and in HESC lysates were examined by ELISA measurements and western blotting analysis respectively. The expression of ARmRNA in HESCs RNA was analyzed by RT-PCR. Testosterone significantly inhibited MMP-1 in both cultured media and cell lysates in a dosedependent manner. Progestins also inhibited MMP-1. Furthermore, FLU completely recovered the decrease of MMP-1 induced by testosterone. ARmRNA was detected in all HESCs RNA. The present study demonstrated that the secretion and production of MMP-1 in HESCs in vitro were inhibited by testosterone through androgen receptors in a manner similar to that seen for progesterone. These findings indicate that androgen may play an important role in morphological and functional changes of human endometrium.

show abstract

“…Андрогены могут усиливать синтез собственных рецепторов. Прогестерон не только не усиливает синтез собственных рецепторов, но и подавляет их, также подавляет и синтез рецепторов эстради-ола [11][12][13][14][15].…”

unclassified

New approaches to the estimation of endometrial dysfunction

et al. 2017

View full text Add to dashboard Cite

Introduction. The application of modern methods for assessing the morphofunctional state of the endometrium to verify and study the expression of sex steroid hormones, proinflammatory markers and markers of angiogenesis using confocal laser scanning microscopy will allow an objective study of the role of studied markers in the pathogenesis of the endometrial dysfunction. The aim of the study was to evaluate the expression of ER and PR receptors in endometrium in patients with endometrial dysfunction. Material and methods. Endometrial biopsy specimens obtained with the aid of a pile-biopsy or a scraping from the uterine cavity are used to conduct the method of immunofluorescent confocal laser scanning microscopy. It is possible to use both cryostat material and paraffin blocks to provide the immunohistochemical analysis. Monoclonal antibodies to ER (1 : 60, Dako, Denmark) and PR (1 : 50, Dako, Denmark) are used as primary antibodies, antibodies conjugated with fluorochrome Alexa Fluor 647 (1 : 1000, Abcam, England) are used as secondary antibodies). Hoechst 33258 (Sigma, USA) is used for staining of cell nuclei. Results. A method of confocal laser scanning microscopy makes it possible to conduct qualitative and quantitative evaluation of the studied markers in different structures of the endometrium.

show abstract

Androgen, estrogen and progesterone receptor expression in the human uterus during the menstrual cycle

Cited by 113 publications

References 17 publications

Analysis of spatial and temporal expression patterns of bone morphogenetic protein family members in the rat uterus over the estrous cycle

Analysis of spatial and temporal expression patterns of bone morphogenetic protein family members in the rat uterus over the estrous cycle

Testosterone inhibits matrix metalloproteinase-1 production in human endometrial stromal cells in vitro

New approaches to the estimation of endometrial dysfunction

Contact Info

Product

Resources

About