Androgen receptor reverses the oncometabolite R-2-hydroxyglutarate-induced prostate cancer cell invasion via suppressing the circRNA-51217/miRNA-646/TGFβ1/p-Smad2/3 signaling

Xu, Hua; Sun, Yin; You, Bosen; Huang, Chi Ping; Ye, Dingwei; Chang, Chawnshang

doi:10.1016/j.canlet.2019.12.014

Cited by 47 publications

(34 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…New insights given to miRNAs and their interactions with different signaling cascades have become more and more significant, as they are chief modulators of almost all cells' machinery [108]. Recent literature has shed light on the fact that these micro managers could have broader implications in AR receptor signaling and can be used as feasible diagnostic or therapeutic options for PC treatment [109]. AR signaling abrogation has been responsible for tumor growth and metastasis.…”

Section: Discussionmentioning

confidence: 99%

“…AR signaling abrogation has been responsible for tumor growth and metastasis. Several studies have delineated the role of miRNAs in AR signaling regulation at various points so that miRNAs can be targeted to restore the normal AR signaling [39,109]. In addition, serum miRNAs levels can also be used as possible diagnostic and prognostic markers for early detection and recurrence of disease [39].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Targeting androgen receptor signaling with MicroRNAs and Curcumin: a promising therapeutic approach for Prostate Cancer Prevention and intervention

et al. 2021

View full text Add to dashboard Cite

Prostate cancer (PC) is a multifactorial disease characterized by the abrogation of androgen receptor signaling. Advancement in microbiology techniques has highlighted the significant role of microRNAs (miRNAs) in the progression of PC cells from an androgen-dependent to an androgen-independent state. At that stage, prostate tumors also fail to respond to currently practiced hormone therapies. So, studies in recent decades are focused on investigating the anti-tumor effects of natural compounds in PC. Curcumin is widely recognized and now of huge prestige for its anti-proliferative abilities in different types of cancer. However, its limited solubility, compatibility, and instability in the aqueous phase are major hurdles when administering. Nanoformulations have proven to be an excellent drug delivery system for various drugs and can be used as potential delivery platforms for curcumin in PC. In this review, a shed light is given on the miRNAs-mediated regulation of androgen receptor (AR) signaling and miRNA-curcumin interplay in PC, as well as on curcumin-based nanoformulations that can be used as possible therapeutic solutions for PC.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Targeting androgen receptor signaling with MicroRNAs and Curcumin: a promising therapeutic approach for Prostate Cancer Prevention and intervention

et al. 2021

View full text Add to dashboard Cite

show abstract

“…The Smad2/3 signaling pathway has recently been shown to be central to the regulation of adipogenesis [ 55 – 57 ]. In addition, this signaling pathway involved in non-coding RNA has also been shown to play an essential role in the context of cancers, cell differentiation, and migration [ 58 – 60 ]. Interestingly, the relevant signaling pathways whereby tsRNAs modulate cellular functionality have only been analyzed via bioinformatics approaches [ 61 , 62 ], suggesting that much research remains to be conducted in this area.…”

Section: Discussionmentioning

confidence: 99%

A novel tsRNA-16902 regulating the adipogenic differentiation of human bone marrow mesenchymal stem cells

Wang

Mei

et al. 2020

Stem Cell Res Ther

View full text Add to dashboard Cite

Background: Transfer RNA-derived small RNAs (tsRNAs) are a recently discovered form of non-coding RNA capable of regulating myriad physiological processes. The role of tsRNAs in hMSC adipogenic differentiation, however, remains incompletely understood. The purpose of this study was to identify the novel tsRNA-16902 as a regulator of hMSC adipogenic differentiation. Methods: In this study, we conducted transcriptomic sequencing of hMSCs after inducing their adipogenic differentiation, and we were thereby able to clarify the molecular mechanism underlying the role of tsRNA-16902 in this context via a series of molecular biology methods. Results: When we knocked down tsRNA-16902 expression, this impaired hMSC adipogenic differentiation and associated marker gene expression. Bioinformatics analyses further revealed tsRNA-16902 to target retinoic acid receptor γ (RARγ). Luciferase reporter assays also confirmed the ability of tsRNA-16902 to bind to the RARγ 3′untranslated region. Consistent with this, RARγ overexpression led to impaired hMSC adipogenesis. Further analyses revealed that Smad2/3 phosphorylation was increased in cells that either overexpressed RARγ or in which tsRNA-16902 had been knocked down. We also assessed the adipogenic differentiation of hMSCs in which tsRNA-16902 was knocked down and at the same time a Smad2/3 inhibitor was added to disrupt Smad2/3 phosphorylation. The adipogenic differentiation of hMSCs in which tsRNA-16902 was knocked down was further enhanced upon the addition of a Smad2/3 signaling inhibitor relative to tsRNA-16902 knockdown alone. Conclusions: Through a comprehensive profiling analysis of tsRNAs that were differentially expressed in the context of hMSC adipogenic differentiation, we were able to identify tsRNA-16902 as a previously uncharacterized regulator of adipogenesis. tsRNA-16902 is able to regulate hMSC adipogenic differentiation by targeting RARγ via the Smad2/3 signaling pathway. Together, our results may thus highlight novel strategies of value for treating obesity.

show abstract

“…Therefore, circRNA is of great importance as a biomarker for cancer screening, cancer diagnosis, cancer prediction, feedback of treatment, and prognosis. Additionally, circRNA's abnormal expression and the circRNA-miRNA-mRNA regulatory network regulation have been increasingly demonstrated in a variety of tumors, such as circRNA_CAMK2A-miR-615-5p-fibronectin 1 network in lung adenocarcinoma metastasis (Du et al, 2019), circRNA_0006948-miR-490-3p-HMGA2 network in esophageal squamous cell carcinoma (Pan et al, 2019), circRNA_ACAP2-miR-29a/b-3p-COL5A1 network in breast cancer (Zhao et al, 2019), circRNA_51217-miRNA-646-TGFβ1/p-Smad2/3 network in prostate cancer (Xu et al, 2019), etc. At present, there are few reports about the circRNA-miRNA-mRNA regulatory network in GISTs.…”

Section: Discussionmentioning

confidence: 99%

Identification of CircRNA–miRNA–mRNA Regulatory Network in Gastrointestinal Stromal Tumor

et al. 2020

View full text Add to dashboard Cite

Circular RNA (circRNA) abnormal expression and regulation are involved in the occurrence and development of a variety of tumors. However, the role of circRNAs still remains unknown in gastrointestinal stromal tumors (GISTs). In the present study, the differential circRNA expression profile of GISTs was screened by human circRNAs chip and verified by qRT-PCR. The circRNA-miRNA-mRNA regulatory network was constructed using the cytoHubba plugin based on the Cytoscape software. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were performed to explore circRNA functions. Six significantly differential circRNAs were also verified in 20 pairs of GISTs and adjacent tissues by qRT-PCR. The result showed that a total of 543 differentially expressed circRNAs were identified in GISTs, of which 242 were up-regulated and 301 were down-regulated. Additionally, the circRNA-miRNA-mRNA network contained six circRNAs, 30 miRNAs, and 308 mRNAs, and the targeted mRNAs were associated with "regulation of biological process," "intracellular organelle," "protein binding," and enriched in Wnt signaling pathway. Furthermore, qRT-PCR demonstrated that hsa_circRNA_061346, hsa_circRNA_103114, and hsa_circRNA_103870 were significantly up-regulated in GISTs (n = 20), and hsa_ circRNA_405324, hsa_circRNA_406821, and hsa_circRNA_000361 were dramatically down-regulated in GISTs (n = 20). In addition, all of these circRNAs were shown to have high diagnostic values, and most of them were significantly associated with tumor size, mitotic figure, and malignant degrees in GISTs (P < 0.05). Therefore, we concluded that circRNAs were abnormally expressed in GISTs, and the circRNA-miRNA-mRNA regulatory network plays an important role in the occurrence and development of GISTs. Also, the identified six candidate circRNAs might be critical circRNAs and may present as potential diagnostic biomarkers for GISTs.

show abstract

Androgen receptor reverses the oncometabolite R-2-hydroxyglutarate-induced prostate cancer cell invasion via suppressing the circRNA-51217/miRNA-646/TGFβ1/p-Smad2/3 signaling

Cited by 47 publications

References 58 publications

Targeting androgen receptor signaling with MicroRNAs and Curcumin: a promising therapeutic approach for Prostate Cancer Prevention and intervention

Targeting androgen receptor signaling with MicroRNAs and Curcumin: a promising therapeutic approach for Prostate Cancer Prevention and intervention

A novel tsRNA-16902 regulating the adipogenic differentiation of human bone marrow mesenchymal stem cells

Identification of CircRNA–miRNA–mRNA Regulatory Network in Gastrointestinal Stromal Tumor

Contact Info

Product

Resources

About