Androgen transport and receptor mechanisms in testis and epididymis

Functional maturation of the epididymis in the rat

“…At this age, however, dihydrotes¬ tosterone and cytoplasmic receptors appear (Calandra, Podestà, Rivarola & Blaquier, 1974), together with increased levels of androgen-binding protein (ABP) (Hansson et al, 1974), in the caput region, suggesting that the epididymis is already under the influence of androgens. The number of fully differentiated Leydig cells increases by 35 days when plasma concentrations of testosterone and dihydrotestosterone are reported to be rising (Gupta et al, 1975).…”

Hormonal regulation of Sertoli cell function in the rat

“…In his review of the hormonal control of mammalian spermatogenesis, Stein-berger (1971) (Murphy, 1965a, b (Desjardins et al, 1974). Steinberger, 1975) (Hansson et al, 1975a(Hansson et al, , 1976a Another indication that androgens are acting directly on the Sertoli cell has come from the demonstration that androgen receptors appear to be localizedto these cells (Sar et al, 1975 ;Wilson and Smith, 1975 (Sar et al, 1975 ;Smith et al, 1975 …”

Structural changes inducted by follicle-stimulating hormone or dibutyryl cyclic AMP on presumptive Sertoli cells in culture.

“…An increased incorporation of labeled leucine into proteins of Sertoli cells cultured in the presence of FSH or Bt2cAMP has also been observed (20). These data suggest that the FSH effects that are mediated in vivo on testicular protein and RNA synthesis (16) and on androgen binding protein production (7)(8)(9) …”

“…Follicle-stimulating hormone (FSH) increases 3': 5'-cyclic AMP (cAMP) production by preparations enriched in Sertoli cells, but not by germinal or Leydig cells (4)(5)(6). Sertoli cells have also been implicated in the in vivo (7,8) and in vitro (9) osmium tetroxide was 60 min. The preparations were then dehydrated with ethanol, transferred into propylene oxide, and embedded in Epon 812/Araldite.…”