Functional maturation of the epididymis in the rat

Setty, B.S.; Jehan, Q

doi:10.1530/jrf.0.0490317

Cited by 32 publications

(25 citation statements)

References 19 publications

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“…Androgens in both testicular fluid and blood plasma are known to influence the developing epididymis (Alexander 1972;Setty and Jehan 1977). The epididymis itself has also been shown to be capable of synthesizing and metabolizing steroids (Hamilton et al 1969;Hamilton 1972) which are added to the luminal pool.…”

Section: The Influence Of Androgensmentioning

confidence: 99%

“…This suggests that androgens, which are probably of testicular origin, are influencing the development of the proximal region of the epididymis at this age. Also in the rat (Setty and Jehan 1977), early differentiation of the initial segment of the epididymis may be associated with the fact that the initial segment is the first region to be exposed to testicular fluids and therefore increasing concentrations of androgens (Cooper and Waites 1974).…”

Section: The Influence Of Androgensmentioning

confidence: 99%

“…The progressive development of stereocilia along the epididymis in prepubertal rats is thought to be androgen-mediated because treatment with exogeneous testosterone hastens their appearance (Setty and Jehan 1977). Gupta et al (1974) suggested that the delay in development between caput and caudal regions in the rat may be related to a low receptor concentration in the cauda epididymidis of maturing animals and/or a higher requirement for androgens by this region of the duct.…”

Section: The Influence Of Androgensmentioning

confidence: 99%

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Late postnatal development and differentiation of the ductus epididymidis in a dasyurid marsupial (Antechinus stuartii)

Taggart

Temple-Smith

1992

Anat Embryol

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The general histology and ultrastructural features of the developing ductus epididymidis were examined in the brown marsupial mouse, Antechinus stuartii, from April, when males were sexually immature, until August, when the adult males were involved in mating activities, just prior to the annual male die-off. Samples were also examined 3 and 6 months after the August die-off period in males kept in isolation from conspecifics during the prebreeding and breeding periods. In April, tubule diameter and epithelial height were largest in the caput and least in caudal segments but the reverse was observed thereafter. Epithelial height increased in caput segments in August and remained high in the post die-off samples. However, caput epithelial height and tubule diameters were low compared with the remainder of the duct from July until February. Luminal shape in caudal segments (10, 11 and 12) changed in June from circular to a narrow slit, and the epithelium became variable in height. The epididymal epithelium was undifferentiated with few cytoplasmic organelles in April. Differentiation occurred mostly from May to June in association with an increased abundance of cytoplasmic organelles, increasing prostatic weight and rising plasma androgen levels. Differentiated principal and basal cells were found in caput and corpus regions in May and in caudal segments in June in association with the de novo development of a brush border of microvilli. Few clear cells were seen in caput and corpus regions of the duct in May but they, and mitochondria-rich cells, were common throughout the duct from June. Development of the unusual structural features of the cauda epididymidis preceded the arrival of spermatozoa in June. The presence of degenerating spermatozoa and cytoplasmic droplets in the cauda at this time suggested that it was not yet capable of supporting sperm viability. There was no evidence to suggest that the presence of spermatozoa has a stimulatory effect on the epididymis. Intact sperm were observed throughout the duct from July. Free cytoplasmic droplets, which showed some evidence of degeneration, collected in large masses in the distal corpus/proximal cauda epididymidis of adult males between aggregates of spermatozoa. Epididymal differentiation appeared complete by mid-July; few ultrastructural changes occurred after this time. Recruitment of spermatozoa into the epididymis ceased by August and was associated with a rapid decline in sperm content in the proximal caput segments. In the November and February samples, spermatozoa were present only in distal corpus and proximal cauda segments.(ABSTRACT TRUNCATED AT 400 WORDS)

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Section: The Influence Of Androgensmentioning

confidence: 99%

Section: The Influence Of Androgensmentioning

confidence: 99%

Section: The Influence Of Androgensmentioning

confidence: 99%

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Late postnatal development and differentiation of the ductus epididymidis in a dasyurid marsupial (Antechinus stuartii)

Taggart

Temple-Smith

1992

Anat Embryol

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“…These cells are involved in absorptive developed in our laboratory for the culture of rat [clear cells (38) Because the principal cells have been shown to synthesize and secrete acidic epididymal glycoprotein (13) and this cell type predominates in the rat epididymal epithelium, it was thought that a specific acidic epididymal glycoprotein antiserum could be utilized for immunocytochemical characterization of cells present in primary cultures established from whole epididymides or major anatomical segments of pubertal and old adult rats. The use of pubertal rats was based on the observation that the pubertal rat epididymal epithelium is active in the secretion of glycerylphosphorylcholine and sialic acid (42), two products associated with the functional maturation of the epididymis (43,44) [3H]Thymidine-labeled cells exhibit structural characteristics similar to those observed in acidic epididymal glycoprotein immunoreactive epididymal epithelial cells. This correlative evidence leads to the conclusion that cultured epididymal epithelial cells established from both pubertal and adult rats can proliferate while retaining their capability to produce and secrete acidic epididymal glycoprotein.…”

Section: Growth Pattern Of Epididymal Epithelial Cells In Culturesmentioning

confidence: 99%

Isolation, culture, and immunocytochemical characterization of epididymal epithelial cells from pubertal and adult rats.

Kierszenbaum¹,

Lea²,

Petrusz³

et al. 1981

Proc. Natl. Acad. Sci. U.S.A.

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A method for the isolation and culture of epididymal e ithelial cells obtained from pubertal and old adult rats is d red. This method permits the establishment of primary cultures of these cells in monolayers from aggregates isolated from whole epididymides and major epididymal anatomical segments (caput, corpus, and cauda) after trypsin and collagenase digestions. A large number of cultured epididymal cells retain a differentiated function as demonstrated by the immunocytochemical and radioimmunoassay finding of acidic epididymal glycoprotein, a spermatozoa-coating protein secreted by the principal cells of rat epididymis. The proliferative tential of cultured epididymal cells obtained from pubertal an old adult donors can be documented by [3H]thymidine labeling and mitotic indices without significant loss of gene expression for acidic epididymal glycoprotein.Results of this study demonstrate that epididymal epithelial cells, consisting of a predominant population of principal cells, can be isolated, cultured, and maintained for up to 3 months.

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“…1982], The principal and basal cells of the initial segment of the rat have shown an earlier peak of mitotic activity than the lower segments [Sun and Flickinger. 1982] and cellular differentiation proceeds from caput to cauda [Setty andJehan. 1977, Sun andFlickinger, 1979], while in the bull I960], the boar [Wrobel and Fallenbacher.…”

Section: Introductionmentioning

confidence: 99%

Postnatal Development of Lectin-Binding Pattern in the Rat Testis and Epididymis

Arya¹,

Vanha‐Perttula²

1986

Cells Tissues Organs

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Seven rhodamine-conjugated lectins were utilized to study the distribution of glycoproteins in the developing rat testis and epididymis. In the testis a clear developmental pattern was found in Ley dig cells and the cell boundaries between Sertoli and spermatogenic cells, as well as during acrosome formation. Some of the first degenerating meiotic cells and the apical extensions of the Sertoli cells at the time of spermiation also displayed a characteristic lectin binding. The epididymal differentiation was characterized by an increasing lectin binding of the subapical Golgi zone and apical surface, and intratubular secretion prior to the arrival of sperm. After the accumulation of tubular secretion and sperm some epithelial cells were transformed into narrow (initial segment) and light cells (distal caput, cauda) with a strong affinity for some lectins. These cells appeared to be responsible for the absorption and digestion of tubular material derived from the testis and of surplus secretion and/or sperm structures.

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Functional maturation of the epididymis in the rat

Abstract: Summary. Weight, histological and biochemical changes in the rat epididymis were investigated during prepubertal, pubertal and postpubertal periods. The

Cited by 32 publications

References 19 publications

Late postnatal development and differentiation of the ductus epididymidis in a dasyurid marsupial (Antechinus stuartii)

Late postnatal development and differentiation of the ductus epididymidis in a dasyurid marsupial (Antechinus stuartii)

Isolation, culture, and immunocytochemical characterization of epididymal epithelial cells from pubertal and adult rats.

Postnatal Development of Lectin-Binding Pattern in the Rat Testis and Epididymis

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