Androgenic effects of a Canadian bleached kraft pulp and paper effluent as assessed using threespine stickleback (Gasterosteus aculeatus)

Wartman, C. A.; Hogan, Natacha S.; Hewitt, L. Mark; McMaster, Mark E.; Landman, Michael J.; Taylor, Sean; Kovács, Tibor; Heuvel, Michael R. van den

doi:10.1016/j.aquatox.2009.02.003

Cited by 30 publications

(26 citation statements)

References 44 publications

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“…The mean EROD activity levels reported here (1-13 pmol min -1 mg protein -1 ) are comparable with the lower range of EROD activities, of between 2.6 and 43.3 pmol min -1 mg protein -1 , that have been reported for sticklebacks exposed to varying degrees of chemical contamination (Sanchez et al, 2007;Wartman et al, 2009). …”

Section: Erod Activitysupporting

confidence: 88%

Effects of sewage effluent remediation on body size, somatic RNA: DNA ratio, and markers of chemical exposure in three-spined sticklebacks

Pottinger

Cook

Jürgens

et al. 2011

Environment International

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Body mass, fork length, RNA:DNA ratio, specific growth rate, and hepatic EROD activity and CYP1A expression, were measured in three-spined sticklebacks in the River Ray (south west England) at sites downstream of an urban waste water treatment works (WWTW) prior to, and following, remediation of the effluent with granular activated carbon (GAC) tertiary treatment.During the same two-year period fish were also sampled from a neighbouring reference river (R.Ock). The WWTW effluent elevated water temperatures and nutrient content in the R. Ray and rendered a direct comparison of fish populations in the two rivers untenable. Instead, the stability of population parameters within each river during matched pre-and post-remediation periods was compared. Stickleback populations in both rivers were annual but fish in the R. Ray spawned earlier and were larger than those in the R. Ock. In the R. Ray fish gained mass throughout the winter months whereas in the R. Ock growth was much reduced during this period. In fish from the R. Ray the somatic RNA:DNA ratio remained elevated during May-November after remediation, rather than declining as in the same period pre-remediation and as was the case for fish in the R. Ock during both periods. The specific growth rate of the first post-remediation generation of sticklebacks in the R. Ray was higher than that of the previous pre-remediation generation.Following remediation there was no decline in hepatic EROD activity or in the abundance of hepatic CYP1A transcripts in fish in the R. Ray suggesting that the primary route of exposure to contaminants for these fish was not via the water column, and that the change in performance of the fish post-remediation was not impeded by continued exposure to contaminants. Both EROD activity and CYP1A expression increased in fish in the R. Ock during the later stages of the study suggesting that the fish in this river were exposed to an unidentified contaminant episode. This may have been linked with the poorer performance of fish in the R. Ock during the post-remediation period. The improved performance of fish in the R. Ray suggest that there may be factors in good quality secondary treated sewage effluent which can adversely influence the performance of fish populations, directly or indirectly, and which can be removed by tertiary treatment.3

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Section: Erod Activitysupporting

confidence: 88%

Effects of sewage effluent remediation on body size, somatic RNA: DNA ratio, and markers of chemical exposure in three-spined sticklebacks

Pottinger

Cook

Jürgens

et al. 2011

Environment International

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“…EROD activity is a sensitive ecotoxicological biomarker of exposure to many traditional xenobiotics, including discharge from the chemical wood industry (Andersson et al 1988, Goksoyr & Förlin 1992, Oikari & Holmbom 1996, van der Oost et al 2003, van den Heuvel 2006, Wartman et al 2009). Chemicals which activate the aryl hydrocarbon receptor (AhR) and induce the cytochrome P450 1A (CYP1A) system can be detected as increased hepatic EROD activity.…”

Section: Erod Activity Of Fishmentioning

confidence: 99%

“…Also retene (7-isopropyl-1-methylphenantrene) causes similar toxic effects on fish as the dioxins (Guiney et al 1997. Retene is a transformation product of resin acids (Tavendale et al 1997, which are continuously discharged from chemical wood industry , Wartman et al 2009). Although activated wastewater treatment efficiently remove most of the resin acids and other wood extractives , pulp and paper production is continuously increasing worldwide (Forström et al 2006) which may increase released volume of harmful compounds.…”

Section: Introductionmentioning

confidence: 99%

Caddis larvae (Trichoptera, Hydropsychidae) indicate delaying recovery of a watercourse polluted by pulp and paper industry

Ratia

Vuori

Oikari

2012

Ecological Indicators

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“…This hypertrophy can be quantified using the Kidney Epithelium Height (KEH) measurement previously developed by (Borg 1993) or the quantification of spiggin production using an ELISA (Katsiadaki et al 2002;Sanchez et al 2008a). Due to this physiological property, the stickleback is considered as a relevant model fish species to assess the (anti)androgenic effects of endocrine-disrupting chemicals (EDCs) in laboratory and field studies (Sanchez et al 2008b;Katsiadaki et al 2006;Pettersson et al 2007;Wartman et al 2009). Chemical analysis has led to the identification of chemicals involved in this biological activity and showed that natural androgenic compounds such as androstenedione, equol, dehydrotestosterone or androsterone represent a large part of measured activity (Hill et al 2010;Thomas et al 2002;Kinani et al 2009).…”

Section: Introductionmentioning

confidence: 99%

“…Chemical analysis has led to the identification of chemicals involved in this biological activity and showed that natural androgenic compounds such as androstenedione, equol, dehydrotestosterone or androsterone represent a large part of measured activity (Hill et al 2010;Thomas et al 2002;Kinani et al 2009). Field studies have described physiological changes associated with androgen exposure such as spiggin induction in stickleback from contaminated sites (Sanchez et al 2008b;Katsiadaki et al 2006;Pettersson et al 2007;Wartman et al 2009) or elongated anal fin rays, resembling the male gonopodium, in female mosquitofish (Gambusia sp.) collected downstream of a pulp mill effluent discharge (Bortone et al 1989;Howell and Denton 1989).…”

Section: Introductionmentioning

confidence: 99%

A potential biomarker of androgen exposure in European bullhead (Cottus sp.) kidney

Villeret

Jolly

Wiest³

et al. 2012

Fish Physiol Biochem

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The aim of this study was to identify a signal that could be used as an androgen exposure indicator in the European bullhead (Cottus sp.). For this purpose, the ultra-structure of the kidney was characterized to identify normal structure of this organ, and histological changes previously described in the kidney of breeding male bullheads were quantified using the Kidney Epithelium Height (KEH) assay previously developed and validated for the stickleback. In the next step, the effect of trenbolone acetate (TbA), a model androgen, was assessed to identify potential androgenic regulation of bullhead kidney hypertrophy.Measurement of KEH performed on adult non-breeding male and female bullheads exposed for 14 and 21 days to 0, 1.26 and 6.50 µg/L showed that kidney hypertrophy is induced in a dose-dependent manner, confirming the hypothesis that the European bullhead possesses a potential biomarker of androgen exposure. Combined with the wide distribution of the European bullhead in European countries and the potential of this fish species for environmental toxicology studies in field and laboratory conditions, the hypothesis of a potential biomarker of androgen exposure offers interesting perspectives for the use of the bullhead as a relevant sentinel fish species in monitoring studies. Inducibility was observed with high exposure concentrations of TbA. Further studies are needed to identify molecular signals that could be more sensitive than KEH.

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Androgenic effects of a Canadian bleached kraft pulp and paper effluent as assessed using threespine stickleback (Gasterosteus aculeatus)

Cited by 30 publications

References 44 publications

Effects of sewage effluent remediation on body size, somatic RNA: DNA ratio, and markers of chemical exposure in three-spined sticklebacks

Effects of sewage effluent remediation on body size, somatic RNA: DNA ratio, and markers of chemical exposure in three-spined sticklebacks

Caddis larvae (Trichoptera, Hydropsychidae) indicate delaying recovery of a watercourse polluted by pulp and paper industry

A potential biomarker of androgen exposure in European bullhead (Cottus sp.) kidney

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