Andrographolide protects rat hepatocytes against paracetamol-induced damage

Visen, P. K. S.; Shukia, Binduja; Patnaik, G. K.; Dhawan, B. N.

doi:10.1016/0378-8741(93)90058-d

Cited by 124 publications

(82 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Protection against paracetamol-induced toxicity has been used as a test for potential hepatoprotective activity by several investigations [26][27][28][29][30] . Liver is the most important and main part of the animal body.…”

Section: Resultsmentioning

confidence: 99%

Hepatoprotective Activity of Aqueous Ethanolic Extract of Chamomile capitula in Paracetamol Intoxicated Albino Rats

Gupta¹,

Misra²

2006

American J. of Pharmacology and Toxicology

View full text Add to dashboard Cite

Hepatoprotective activity of aqueous ethanolic extract of Chamomile recutita capitula against paracetamol induced hepatic damage in albino rats was observed. In the present study the effect of aqueous ethanolic extract of Chamomile recutita capitula on blood and liver glutathione, Na + K + -ATPase activity, serum marker enzymes, serum bilirubin, glycogen and thiobarbutiric acid reactive substances against paracetamol induced damage in rats have been studied to find out the possible mechanism of hepatoprotection. It was observed that extract of chamomile has reversal effects on the levels of above-mentioned parameters in paracetamol hepatotoxicity. The extract of capitula of chamomile functions as a hepatoprotective agent and this hepatoprotective activity of chamomile may be due normalization of impaired membrane function activity.

show abstract

Section: Resultsmentioning

confidence: 99%

Hepatoprotective Activity of Aqueous Ethanolic Extract of Chamomile capitula in Paracetamol Intoxicated Albino Rats

Gupta¹,

Misra²

2006

American J. of Pharmacology and Toxicology

View full text Add to dashboard Cite

show abstract

“…21,22) Leaf extract of Andrographis paniculata and andrographolide were found to be effective in preventing carbontetrachlorideinduced liver damage in rats and mice. 17,25,27) Andrographolide also showed significant hepatoprotective effect against various types of liver damage induced by galactosamine 18,23,24) or paracetamol, 17,28) and had a higher capability than a classical antioxidant silymarin in preventing a decrease of bile production induced by paracetamol. 18) Andrographolide played a hepatoprotective role by reducing a lipid peroxidation product malondialdehyde (MDA), in which the lowering of MDA formation conveyed the free radical scavenging property of diterpene lactones of andrographolide, as well as by maintaining high level of glutathione, glutamicpyruvate transaminase, and alkaline phosphatase in carbontetrachloride or tert-butylhydroperoxide treated mice.…”

Section: Hepatoprotective Activitymentioning

confidence: 99%

“…5) In Thailand, this plant was selected by the Ministry of Public Health as one of the medicinal plants to be included in "The National List of Essential Drugs A.D. 1999" (List of Herbal Medicinal Products). 6) Extensive research has revealed that Andrographis paniculata has a surprisingly broad range of pharmacological effects and some of them are extremely beneficial, such as antiinflammatory, [7][8][9][10][11] antidiarrhoeal, 12,13) antiviral, 14) anti-malarial, 15,16) hepatoprotective, [17][18][19][20][21][22][23][24][25][26][27][28][29] cardiovascular, 30,31) anticancer, [32][33][34][35][36][37][38][39] and immunostimulatory [40][41][42][43][44] activities. On the other hand, male reproductive toxicity 45) and cytotoxicity 46) of this plant have been reported as well.…”

Section: Introductionmentioning

confidence: 99%

Pharmacological Aspects of Andrographis paniculata on Health and Its Major Diterpenoid Constituent Andrographolide

Jarukamjorn

Nemoto

2008

JOURNAL OF HEALTH SCIENCE

201

111

View full text Add to dashboard Cite

Nowadays, research on medicinal plants has attracted a lot of attention globally. A number of evidence has been accumulated to demonstrate promising potential of medicinal plants used in various traditional, complementary, and alternative systems. In recent years, a medicinal plant, Andrographis paniculata, and its major active phytochemicals have been extensively studied for several pharmacological activities. To understand the mechanism of action, researches have to be carried out at molecular levels. The present review aims at compiling consequential compendium of pharmacological benefits of health on this plant and its major diterpenoid constituent andrographolide that have been tested in various experimental models using modern scientific methodologies.

show abstract

“…1), is one of the main active constituents of Andrographis paniculate (BURM) NEES, a famous traditional Chinese medicine. Andrographolide has many bioactivities, such as antiinflammatory and antimicrobial, 1,2) antiplatelet aggregation, 3,4) hepatoprotective, 5,6) and anti-human immunodeficiency virus (HIV) activity. 7) Andrographolide is widely used clinically with good results, but it did not show efficacy in activity assays in vitro.…”

mentioning

confidence: 99%

Six New Andrographolide Metabolites in Rats

Gao

et al. 2003

CHEMICAL & PHARMACEUTICAL BULLETIN

View full text Add to dashboard Cite

Andrographolide chemically designated as 2(3H)-furanone, 3-[2-[decahydro-6-hydroxy-5-(hydroxymethyl)-5,8a-dimethyl-2-methylene-1-napthalenyl] ethylidene] dihydro-4-hydroxy ( Fig. 1), is one of the main active constituents of Andrographis paniculate (BURM) NEES, a famous traditional Chinese medicine. Andrographolide has many bioactivities, such as antiinflammatory and antimicrobial, 1,2) antiplatelet aggregation, 3,4) hepatoprotective, 5,6) and anti-human immunodeficiency virus (HIV) activity. 7) Andrographolide is widely used clinically with good results, but it did not show efficacy in activity assays in vitro. Andrographolide has aroused the interest of many pharmacologists, and numerous experiments have been performed, although much remains to be clarified. A few investigations of the pharmacokinetics have been reported after oral administration of andrographolide. 8,9) However, to the best of our knowledge, there has been no report on its metabolites either in humans or animals. We isolated and analyzed the metabolites of andrographolide in blood, urine, bile, and the contents of the small intestine and stomach of rats. So far, we have identified 10 new metabolites. In this paper, six new metabolites are presented including their extraction, isolation, and identification.Metabolite 5 (M-5), colorless powder, melting point Ͼ300°C (decomposed), was positive for the Legal and Kedde reactions, suggesting the presence of an a,b-unsaturated lactone. The IR spectrum showed the presence of hydroxyl (3433 cm Ϫ1 ), ester carbonyl (1744 cm Ϫ1 ), carbon-carbon double bond (1643 cm Ϫ1 ), and sulfonate (1205 cm Ϫ1 ) groups in the molecule. In the high-resolution second ionization mass spectrum (HR-SI-MS), a quasimolecular peak [MϪH] Ϫ at m/z 493.1205 (Calcd 493.1207) was seen, and thus the molecular formula of C 20 H 30 O 10 S 2 was derived.Wide variation in the chemical shifts at C 2 , C 3 , C 9 , and C 11 -C 16 were observed, while the other carbons were almost the same when metabolite 5 was compared with the parent drug andrographolide, suggesting that the difference of metabolite 5 occurred at the side chain of lactone and ring A. The carbon signal of carbonyl shifted from 172.6 to 176.7 ppm, while the oxygen-linked carbon of C-15 shifted from 76.1 to 72.7 ppm. The changes in chemical shifts showed that the double bond at 12(13) of andrographolide changed to the 13(14) double bond, by which the double bond transferred from the outside to the inside of the lactone ring. In the heteronuclear multiple bond connectivity (HMBC) spectrum, the signal of H-15 (4.94, 2H) had peaks correlated with C-13 (131.3 ppm) and C-14 (151.7 ppm), indicating that a carboncarbon double bond is located at 13(14). The singlet of H-14 (7.73, H, br s) had a cross-peak with 55.8 (C-12), and H-12 (3.97, H, br d, Jϭ10.2 Hz) correlated with 131.3 (C-13), 151.7 (C-14), and 176.7 ppm (C-16). These correlated peaks showed that the sulfonate group is linked at C-12.The carbon signals of metabolite 5 at ring A differed from those of andrographolide. The...

show abstract

Andrographolide protects rat hepatocytes against paracetamol-induced damage

Cited by 124 publications

References 8 publications

Hepatoprotective Activity of Aqueous Ethanolic Extract of Chamomile capitula in Paracetamol Intoxicated Albino Rats

Hepatoprotective Activity of Aqueous Ethanolic Extract of Chamomile capitula in Paracetamol Intoxicated Albino Rats

Pharmacological Aspects of Andrographis paniculata on Health and Its Major Diterpenoid Constituent Andrographolide

Six New Andrographolide Metabolites in Rats

Contact Info

Product

Resources

About