Anethum graveolens L‎. Alleviates Sperm ‎Damage by Limiting Oxidative Stress ‎and Insulin ‎Resistance ‎in ‎Diabetic Rats ‎

Abstract

Background: It has been reported that diabetes is associated with sperm ‎damage and infertility. Objective: The purpose of this experiment was to survey the effect of Anethum graveolens L. (Dill) powder on sperm profiles, oxidative stress, insulin resistance, and histological changes in male diabetic rats. Methods: Male rats were randomly divided into 6 groups (n=7); group 1: normal rats, 2: normal rats + 100mg/kg Dill, 3: normal rats + 300mg/kg Dill, 4: diabetic rats, 5: diabetic rats + 100mg/kg Dill, and 6: diabetic rats + 300mg/kg Dill. After 2 months of treatments, the sperm profile, anti-oxidant activity, superoxide dismutase (SOD) activity, and malondialdehyde were measured. The histopathology of testis was evaluated. Hormonal changes and tumor necrosis factor-α (TNF-α) levels were measured by ELISA. Results: Total anti-oxidant and SOD activity in diabetic rats significantly decreased, while MDA concentration was significantly increased in the testis and pancreas of diabetic rats compared with control. However, the use of Dill significantly normalized these profiles. The treatment of diabetic rats with Dill changed the sperm parameters. The levels of testosterone, FSH, and LH in diabetic rats were significantly reduced, but the treatment with Dill did not alter the level of these hormones. Dill also significantly normalized testis morphological changes, insulin resistance, and inflammation. Conclusion: The use of Dill normalized oxidative stress, inflammation, and insulin resistance in diabetic rats that correlated with sperm profile and testis histological changes. The treatment of diabetic rat models with Dill did not show harmful effects on sperm profiles.

Favorable effects of Anethum graveolens on liver oxidative stress and cholesterol 7 alpha-hydroxylase levels in non-alcoholic fatty liver disease (NAFLD) rat models

“…The minimum number of animals was used, safety procedures were compiled in all mandatory experimental works, and minimum suffering of animals was considered in all experiments. The concentration of dill tablet (300 mg/kg) which was used in the present study was based on the previously described optimal concentration [ 13 , 18 ]. Other report indicated that the 300 mg/kg dill hydroalcoholic extract is effective dose [ 17 ].…”

“…Anethum graveolens (dill) is one of the traditional herbal plant with many useful effects [ 10 , 11 ]. Dill is recognized as a rich source of phenolic, flavonoids, saponin, cardiac glycosides and terepene [ 12 , 13 ]. These components have potential antioxidant and hypolipidemic effects [ 13 , 14 ].…”

“…Dill is recognized as a rich source of phenolic, flavonoids, saponin, cardiac glycosides and terepene [ 12 , 13 ]. These components have potential antioxidant and hypolipidemic effects [ 13 , 14 ]. It has been established that administration of essential oil, leaf and seed of dill in animal models considerably normalized lipid profile including TG, TC, LDL-C, VLDL-C, and restored glucose levels and significantly raised high-density lipoprotein (HDL) cholesterol levels [ 15 , 16 ].…”

Importance of hyperglycemia in COVID-19 intensive-care patients: Mechanism and treatment strategy

“…These findings propose that appropriate supplementation of these vitamins may increase our resistance to COVID-19 infection. Antioxidant supplementation is potentially recommended in COVID-19 patients with primary underlying diseases, such as hypertension, diabetes, coronary heart disease, and cancer [ 7 , [45] , [46] , [47] ].…”

Memory and Learning Improvement by Resveratrol and Probiotics via the ‎Gut-Brain Axis and Antioxidant Activity in Diabetic Rats

“…Diabetes is a chronic metabolic disease and is characterized by insulin resistance and loss of pancreatic β-cell function [1,2]. Hyperglycemia is one of the central factors for the development of oxidative stress that induces important complications in diabetic patients [3].…”