Angelica polysaccharide attenuates LPS-induced inflammation response of primary dairy cow claw dermal cells via NF-κB and MAPK signaling pathways

Abstract

Background Laminitis, an inflammation of the claw laminae, is one of the major causes of bovine lameness, which can lead to enormous economic losses and animal welfare problems in dairy farms. Angelica polysaccharide (AP) is proved to possess anti-inflammatory properties. But the role of AP on inflammatory response of the claw dermal cells has not been reported. The aim of this study was to investigate the anti-inflammatory effects of AP on lipopolysaccharide (LPS)-induced primary claw dermal cells of dairy cow and clarify the potential mechanisms. In the current research, the primary claw dermal cells were exposed to gradient concentrations of AP (10, 50, 100 µg/mL) in the presence of 10 µg/mL LPS. The levels of cytokines and nitric oxide (NO) were detected with ELISA and Griess colorimetric method. The mRNA expressions of TLR4, MyD88 and chemokines were measured with qPCR. The activation of NF-κB and MAPK signaling pathways was detected with western blotting. Results The results indicated that AP reduced the production of inflammatory mediators (TNF-α, IL-1β, IL-6 and NO), downregulated the mRNA expression of TLR4, MyD88 and some pro-inflammatory chemokines (CCL2, CCL20, CXCL2, CXCL8, CXCL10), and suppressed the NF-κB and MAPK signaling pathways evidenced by inhibition of the phosphorylation of IκBα, p65 and ERK, JNK, p38. Conclusions Our results demonstrated that AP may exert its anti-inflammatory effects on claw dermal cells of dairy cow by regulating the NF-κB and MAPK signaling pathways.

Characterization of the Bacterial Community of Rumen in Dairy Cows with Laminitis

“…Bovine laminitis, one of the most costly lameness conditions, is an economic drain on producers [ 26 ]. It is generally accepted that the micro-circulation of a blood disorder within the corium induced by microbiota metabolites, such as LPS, lactate, and histamine, is the main pathogenesis of laminitis [ 10 , 11 , 12 ]. Thus, in the present study, we detected the characteristics of rumen microbiota and the concentration of LPS, lactic acid, and histamine in the plasma from cows from control and laminitis groups.…”

“…Although laminitis has attracted enough attention, its physiopathology remains unelucidated. Currently, it is commonly considered as the result of the interaction of various factors, including breeding conditions and metabolic insults, which increase the accumulation of toxic substances, such as histamine, lactic acid, and lipopolysaccharide (LPS), leading to vascular lesions and degradation of the suspensory apparatus of the third phalanx within the digit [ 10 , 11 , 12 ].…”

Clinical study of Tongdu Shujin decoction in the treatment of ankylosing spondylitis with cold-dampness obstruction type: Study protocol for a randomized controlled trial

“…[ 27 ] Chuanxiong and angelica extracts exert anti-inflammatory effects by hindering inflammatory signaling. [ 28 , 29 ] The active ingredient of Safflower, safflower yellow, has anti-inflammatory, analgesic, and immune regulation functions. [ 30 ] Both clematis chinensis and angelicae pubescentis radix extract exert anti-inflammatory effects by reducing pro-inflammatory cytokine levels.…”

Anti-inflammation of isoliquiritigenin via the inhibition of NF-κB and MAPK in LPS-stimulated MAC-T cells

“…MAPKs, consisting of p38, ERK and JNK, are involved in a cascade regulating NF-κB-mediated transcription of the proinflammatory cytokines, i.e. IL-6, IL-1β and TNF-α, and inflammatory mediators, such as NO, iNOS, COX-2 and prostaglandin E2 [ 24 , 34 , 35 ]. Previous reports indicated that ISL inhibited MAPK activities in human liver cancer and colitis [ 36 , 37 ].…”