Angiogênese e doenças da retina

Damico, Francisco Max

doi:10.1590/s0004-27492007000300030

Cited by 18 publications

(12 citation statements)

References 61 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Angiogenesis is mediated by a huge number of stimulatory and inhibitory factors. An imbalance between these molecules occurs in pathological conditions [Damico, 2006]. Vascular endothelial growth factor (VEGF) is a crucial pro‐angiogenic factor and the main promotor of endothelial cell (EC) growth, migration, and vessel dilation and permeability.…”

mentioning

confidence: 99%

“…AMD is the leading cause of blindness in individuals over 50 years in developed countries, increasing with age [Wang et al, 2004], and affecting approximately 50 million people worldwide [Ferguson and Apte, 2008]. AMD‐related visual loss is a complex process starting by the deposition of debris in the outer retina [Damico, 2006; Andreoli and Miller, 2007]. The deposition of insoluble material, the calcification and increase in thickness of Bruch's membrane, and a less fenestrated and thinner choriocapillaris lead to photoreceptors/retinal pigment epithelium hypoxia resulting in a stimulus for VEGF release [Shibuya, 2001; Adamis, 2005; deJong, 2006; Semenza, 2007].…”

mentioning

confidence: 99%

“…The deposition of insoluble material, the calcification and increase in thickness of Bruch's membrane, and a less fenestrated and thinner choriocapillaris lead to photoreceptors/retinal pigment epithelium hypoxia resulting in a stimulus for VEGF release [Shibuya, 2001; Adamis, 2005; deJong, 2006; Semenza, 2007]. The development of choroidal neovascularization (CNV) results into devastating visual effects for patients [Damico, 2006; Andreoli and Miller, 2007].…”

mentioning

confidence: 99%

“…Although VEGF is not the only factor whose expression is increased in AMD, its effect is necessary and sufficient for the induction of retinal neovascularization [Damico, 2006]. VEGF is able to initiate a cascade of endothelial signaling that acts specifically in terms of survival, proliferation and migration, both in vitro and in vivo [Costa et al, 2004, 2007].…”

mentioning

confidence: 99%

See 3 more Smart Citations

Bevacizumab and ranibizumab on microvascular endothelial cells: A comparative study

Costa

Carneiro

Rocha

et al. 2009

J of Cellular Biochemistry

View full text Add to dashboard Cite

Given its broad effects in endothelium, vascular endothelial growth factor (VEGF) represents the primary rate-limiting step of angiogenesis. Therefore, VEGF targeting therapies were soon developed. Bevacizumab and ranibizumab are two of these therapeutic agents already in clinical use. Bevacizumab was first used for cancer treatment, whereas ranibizumab was designed to target choroidal neovascularization, the main cause of blindness in age-related macular degeneration. The present study aims to compare the multiple effects of bevacizumab and ranibizumab in human microvascular endothelial cells (HMECs). HMEC cultures were established and treated during 24 h with the anti-VEGF agents within the intravitreal-established concentration range or excipients. Analyses of VEGF content in cell media and VEGF receptor-2 (VEGFR-2) expression in cell lysates were performed. No cell cytotoxicity (MTS assay) was found in anti-VEGF-treated cultures at any concentration. Apoptosis (TUNEL assay) was significantly increased and cell proliferation (BrdU assay), migration (transwell assay) and assembly into vascular structures were significantly reduced by incubation with both agents at the two doses used. These findings were accompanied by a strong decrease in VEGF release, and in phosphorylated VEGFR-2 and Akt expression for both agents at the clinical concentration. Interestingly, phosphorylated Erk was only significantly reduced upon bevacizumab treatment. In addition, proliferation was more affected by ranibizumab, whereas migration, capillary formation, and phosphorylated VEGFR2 expression were significantly reduced by bevacizumab as compared to ranibizumab. Therefore, although both agents presented anti-angiogenic actions, distinct effects were exerted by the two molecules in HMEC. These findings suggest that a careful confirmation of these effects in clinical settings is mandatory.

show abstract

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Bevacizumab and ranibizumab on microvascular endothelial cells: A comparative study

Costa

Carneiro

Rocha

et al. 2009

J of Cellular Biochemistry

View full text Add to dashboard Cite

show abstract

“…It is essential for various physiological processes, such as the growth and development of organs, capillary growth, the reproductive cycle and the healing of wounds. On the other hand, it may also contribute to the development of various pathological processes, such as rheumatoid arthritis, diabetic retinopathy, and the growth and metastasis of tumors 1, 2.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Antioxidant and Antiangiogenic Properties of Caesalpinia Echinata Extracts

Gomes¹,

Jimenez²,

Silva³

et al. 2014

J. Cancer

View full text Add to dashboard Cite

Natural products contain important combinations of ingredients, which may to some extent help to modulate the effects produced by oxidation substrates in biological systems. It is known that substances capable of modulating the action of these oxidants on tissue may be important allies in the control of neovascularization in pathological processes. The aim of this study was to evaluate the antioxidant and antiangiogenic properties of an ethanol extract of Caesalpinia echinata. The evaluation of antioxidant properties was tested using two methods (DPPH inhibition and sequestration of nitric oxide). The antiangiogenic properties were evaluated using the inflammatory angiogenesis model in the corneas of rats. The extract of C. echinata demonstrated a high capacity to inhibit free radicals, with IC50 equal to 42.404 µg/mL for the DPPH test and 234.2 µg/mL for nitric oxide. Moreover, it showed itself capable of inhibiting the inflammatory angiogenic response by 77.49%. These data suggest that biochemical components belonging to the extract of C. echinata interfere in mechanisms that control the angiogenic process, mediated by substrates belonging to the arachidonic acid cascade, although the data described above also suggest that the NO buffer may contribute to some extent to the reduction in the angiogenic response.

show abstract

The Angiogenic Peptide Vascular Endothelial Growth Factor–Basic Fibroblast Growth Factor Signaling is Up‐Regulated in a Rat Pressure Ulcer Model

Yang

Wang

Shi

et al. 2013

The Anatomical Record

View full text Add to dashboard Cite

The purpose of this study is to investigate the mRNA and protein expression levels of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) in pressure ulcers, and to elucidate the molecular mechanism by which VEGF and bFGF are involved in pressure ulcer formation. A rat model of ischemia-reperfusion pressure ulcer was established by magnetic disk circulating compression method. Real-time fluorescence quantitative PCR and Western blot assays were conducted to detect the mRNA and protein expression of VEGF and bFGF in the tissues of rat I-, II-, and III-degree pressure ulcers, the surrounding tissues, and normal skin. Our study confirmed that the mRNA and protein expression levels of VEGF and bFGF in the tissues of rat I-degree pressure ulcer were significantly higher than that in the II-and III-degree pressure ulcer tissues (P < 0.05). The expression of VEGF and bFGF in the tissues surrounding I-and II-degree pressure ulcers were higher than the rats with normal skin. The expression of VEGF and bFGF in the tissues of rat III-degree pressure ulcer was lower than that in the surrounding tissues and normal skin (P < 0.05). There was a significant positive correlation between change in the VEGF and bFGF. The results showed that with an increase in the degree of pressure ulcers, the expression of VEGF and bFGF in pressure ulcers tissue are decreased. This leads to a reduction in angiogenesis and may be a crucial factor in the formation of pressure ulcers.

show abstract

Angiogênese e doenças da retina

Cited by 18 publications

References 61 publications

Bevacizumab and ranibizumab on microvascular endothelial cells: A comparative study

Bevacizumab and ranibizumab on microvascular endothelial cells: A comparative study

Evaluation of Antioxidant and Antiangiogenic Properties of Caesalpinia Echinata Extracts

The Angiogenic Peptide Vascular Endothelial Growth Factor–Basic Fibroblast Growth Factor Signaling is Up‐Regulated in a Rat Pressure Ulcer Model

Contact Info

Product

Resources

About