The Angiogenic Peptide Vascular Endothelial Growth Factor–Basic Fibroblast Growth Factor Signaling is Up‐Regulated in a Rat Pressure Ulcer Model

Abstract

The purpose of this study is to investigate the mRNA and protein expression levels of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) in pressure ulcers, and to elucidate the molecular mechanism by which VEGF and bFGF are involved in pressure ulcer formation. A rat model of ischemia-reperfusion pressure ulcer was established by magnetic disk circulating compression method. Real-time fluorescence quantitative PCR and Western blot assays were conducted to detect the mRNA and protein expression of VEGF and bFGF in the tissues of rat I-, II-, and III-degree pressure ulcers, the surrounding tissues, and normal skin. Our study confirmed that the mRNA and protein expression levels of VEGF and bFGF in the tissues of rat I-degree pressure ulcer were significantly higher than that in the II-and III-degree pressure ulcer tissues (P < 0.05). The expression of VEGF and bFGF in the tissues surrounding I-and II-degree pressure ulcers were higher than the rats with normal skin. The expression of VEGF and bFGF in the tissues of rat III-degree pressure ulcer was lower than that in the surrounding tissues and normal skin (P < 0.05). There was a significant positive correlation between change in the VEGF and bFGF. The results showed that with an increase in the degree of pressure ulcers, the expression of VEGF and bFGF in pressure ulcers tissue are decreased. This leads to a reduction in angiogenesis and may be a crucial factor in the formation of pressure ulcers.

Mesenchymal stem cells derived from human iPS cells via mesoderm and neuroepithelium have different features and therapeutic potentials

“…HGF and EGF play a role in skin wound healing [ 47 ]. VEGF-A and bFGF play a role in angiogenesis, and the decreased expression of VEGF-A and bFGF leads to deterioration of pressure ulcer [ 48 ]. These factors thus elucidate the different effects observed herein between the two types of iPSC-derived MSCs in the tested disease models, and our results suggest that some therapeutic effects with MSCs are based on the potential for differential paracrine factor expression.…”

A Novel Model of Human Skin Pressure Ulcers in Mice

“…Additionally, growth factors could be another alternative to stem cells. According to Yang et al [13] , the expression of VEGF and bFGF in PU tissue is decreased. This leads to a reduction in angiogenesis, which may be a crucial factor in the formation of PUs.…”

Triptolide Suppresses Alkali Burn‐Induced Corneal Angiogenesis Along with a Downregulation of VEGFA and VEGFC Expression

“…VEGFA has been well elucidated as a functional endogenous corneal angiogenic factor, indicating a key role in inflammatory angiogenesis (Amano et al, ; Joussen et al, ). VEGFA can directly promote the proliferation, migration, and capillary lumen formation of vascular endothelial cell (Chang et al, ; Yang et al, ). In addition, VEGFA has proteolytic activity which can increase vascular leakage, promoting monocyte chemotaxis and B‐cell production (Ferrara et al, ).…”