Angiogenesis in gliomas.

Abstract

Patients. Ninety-nine patients (54 women and 45 men) aged 18-80 years with clinically diagnosed brain tumour were included in the study. Based on histopathological investigations performed to determine tumour's histological type and malignancy grade, the patients were divided into study groups according to the WHO Abstract: Brain gliomas are characterized by invasive growth and neovascularisation potential. Angiogenesis plays a major role in the progression of gliomas and its determination has a great prognostic value. The aim of the study was to assess the vascularisation of chosen brain gliomas and to estimate how it is correlated with tumour histological type, malignancy grade, location and size, and with age and sex of patients. Tumour vascularisation analysis was based on the determination of microvascular proliferation (MVP) and microvessel density (MVD). Microvascular proliferation was measured with immunohistochemical methods using mouse monoclonal antibodies to detect cell proliferation antigens. The following antibodies were used Ki-67 and PCNA (DAKO). Identification of vessels was performed by CD31 antibody and anti-human von Willebrand factor (DAKO). The highest microvascular proliferation and microvascular density were observed in multiform glioblastomas and the lowest in oligodendrogliomas. Significant correlation was observed between the vascularisation and malignancy grade.

Transmembrane-4-L-six-family-1, a potential predictor for poor prognosis, overexpressed in human glioma

“…The 5-year survival of patients with glioblastoma, which is a highly vascular tumor, is ∼10% [24]. It was reported that an increase in vascularization significantly worsens prognosis in glioma patients [21]. The results in the present study indicated a negative effect of TM4SF1 expression on the prognosis of glioma patients.…”

“…With regard to angiogenesis, glioblastomas are known to have blood vessels of increased diameter with high permeability and highly proliferative ECs [20]. One of the malignancy evaluation criteria is the increased neoplastic proliferation of glial cells running parallel to endothelial vascular proliferation [21]. Vascular density in glioblastoma is markedly higher than that in tumors of lower histological grade [22].…”

Aberrant activation of Hedgehog/Gli1 pathway on angiogenesis in gliomas

“…[8][9][10] Intense angiogenesis is one of the basic biological features of gliomas. [11] Studies have shown that occurrence, development, and even relapse of gliomas are closely correlated with angiogenesis. Therefore angiogenesis is well recognized as an essential mechanism for glioma growth.…”

“…Over secreting vascular endothelial growth factor (VEGF), matrix metalloproteinase 2 (MMP2), and matrix metalloproteinase 9 (MMP9) play an important role during this process. [11][12][13][14][15] In addition, low oxygen levels increase messenger RNA (mRNA) transcription of VEGF, MMP2, and MMP9 in glioma cells by the increase of the stability of hypoxia-inducible factor-1 alpha (HIF-1 α). [11,12,16,17] However, the mechanisms involving in the process are not well known.…”

Magnetic resonance imaging targeting of intracranial glioma xenografts by Resovist-labeled endothelial progenitor cells

“…Sufficient supplies of oxygen and nutrients by the neovessels in gliomas are necessary for tumor survival, infiltration and development [2,3]. In fact, microvasculature density is used as one of the factors for grading gliomas [4]. Additionally, recent studies showed that the possible existence of brain tumor stem cells, which lie within the niche microenvironment of the tumor microvasculature network, may be one of the key factors that induce glioma recurrence [5].…”