Angiogenesis in the course of enucleation-induced adrenal regeneration—Expression of selected genes and proteins involved in development of capillaries

Tyczewska, Marianna; Ruciński, Marcin; Trejter, Marcin; Ziółkowska, Agnieszka; Szyszka, Marta; Malendowicz, Ludwik K.

doi:10.1016/j.peptides.2012.09.025

Cited by 10 publications

(31 citation statements)

References 65 publications

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“…Microarray expression analysis was described earlier [14,56,57,58]. RNA subjected to microarray studies was obtained from the above-described experiments.…”

Section: Methodsmentioning

confidence: 99%

Effect of ACTH and hCG on the Expression of Gonadotropin-Inducible Ovarian Transcription Factor 1 (Giot1) Gene in the Rat Adrenal Gland

Jopek

Tyczewska

Ramanjaneya

et al. 2018

IJMS

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Gonadotropin-inducible ovarian transcription factor-1 (Giot1) belongs to a family of fast-responsive genes, and gonadotropins rapidly induce its expression in steroidogenic cells of ovaries and testes of rats. Gonadal Giot1 gene expression is regulated by cyclic adenosine monophosphate (cAMP) -dependent protein kinase A pathway, with essential role of orphan nuclear receptor NR4A1 transcription factor (nuclear receptor subfamily 4, group A, member 1). A recent study reports that Giot1 is also expressed in adrenals, however, the mechanism of its regulation in adrenal gland is yet to be identified. Therefore, the aim of this study was to characterise the changes in Giot1 gene expression in male and female rat adrenals using wide range of in vivo and in vitro experimental models. Special emphasis was directed at the Giot1 gene regulation by ACTH and gonadotropin. In our study, we found that ACTH rapidly stimulates Giot1 expression both in vivo and in vitro. However, gonadotropin does not affect the adrenal Giot1 gene expression, presumably due to the low expression of gonadotropin receptor in adrenals. Both testosterone and estradiol administered in vivo had inhibitory effect on Giot1 gene expression in the adrenals of post-gonadectomized adult rats. Further, our studies revealed that the intracellular mechanism of Giot1 gene regulation in rat adrenals is similar to that of gonads. As in the case of gonads, the expression of Giot1 in adrenal gland is regulated by cAMP-dependent signaling pathway with essential role of the NR4A1 transcription factor. The results of our studies suggest that Giot1 may be involved in the regulation of rat adrenocortical steroidogenesis.

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“…Microarray expression analysis was described earlier [14,56,57,58]. RNA subjected to microarray studies was obtained from the above-described experiments.…”

Section: Methodsmentioning

confidence: 99%

Effect of ACTH and hCG on the Expression of Gonadotropin-Inducible Ovarian Transcription Factor 1 (Giot1) Gene in the Rat Adrenal Gland

Jopek

Tyczewska

Ramanjaneya

et al. 2018

IJMS

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“…Human prostate culture cells were washed two times using PBS, then the total RNA was extracted using TRI Reagent (Sigma-Aldrich, Poznan, Poland). Total RNA was purified on columns (NucleoSpin Total RNA Isolation, Qiagen GmbH, Hilden, Germany) [19][20][21][22][23]. The amount of total RNA was determined by optical density at 260 nm and its purity was estimated by 260/280 nm absorption ratio (higher than 1.8) (NanoDrop spectrophotometer, Thermo Scientific, Waltham, MA, USA).…”

Section: Methodsmentioning

confidence: 99%

Lack of expression of preproorexin and orexin receptors genes in human normal and prostate cancer cell lines

Szyszka

Paschke

Tyczewska

et al. 2016

Folia Histochem Cytobiol.

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Introduction. Studies on expression of orexins (OXs) and their receptors in human prostate gland and human prostatic cell lines are scanty and their results contradictory. Regarding this, we carefully reinvestigated this problem on human prostatic cell lines. Material and methods. Expression of preproorexin (ppOX) (6 primer pairs), and orexin receptors 1 and 2 (OXR1, OXR2) (4 and 2 primer pairs, respectively) was assessed by conventional PCR and QPCR in human normal (PrEC, PrSc, PrSmC) and prostate carcinoma (Du145, LNCaP, and PC3) cell lines. We designed intron spanning primers and also we applied primers from earlier publications and commercially available ones. Results. With the designed primer pairs, in all studied cell lines we failed to demonstrate expression of ppOX, OXR1 and OXR2 genes at the mRNA level, while reaction products were observed in control tissues (human placenta and adrenals). Primers applied in earlier studies did not form amplification products specific for preproorexin or orexin 1 receptor. Some commercially available primers for orexin receptor 1 produced false positive results. Conclusions. We found no evidence for the presence of preproorexin-orexin receptors system genes' mRNAs in human prostate cell lines. The reported premises for these genes' expression in prostate and prostatic cell lines may have arisen either from the presence of non-prostate cells included in the samples or from faulty PCR settings.

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“…The applied methods were described earlier [1,2,[45][46][47][53][54][55][56]. From collected cells, samples of adrenal zones and from samples of entire adrenal glands, total RNA was extracted using TRI Reagent (Sigma) and, then, purified on columns (Rnasy Mini Kit, Qiagen).…”

Section: Rna Isolationmentioning

confidence: 99%

“…Microarray expression analysis was also described earlier [54][55][56]. RNA subjected to microarray studies was obtained from the above described experiments.…”

Section: Reverse Transcriptionmentioning

confidence: 99%

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Visinin-like peptide 1 in adrenal gland of the rat. Gene expression and its hormonal control

et al. 2015

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Angiogenesis in the course of enucleation-induced adrenal regeneration—Expression of selected genes and proteins involved in development of capillaries

Cited by 10 publications

References 65 publications

Effect of ACTH and hCG on the Expression of Gonadotropin-Inducible Ovarian Transcription Factor 1 (Giot1) Gene in the Rat Adrenal Gland

Effect of ACTH and hCG on the Expression of Gonadotropin-Inducible Ovarian Transcription Factor 1 (Giot1) Gene in the Rat Adrenal Gland

Lack of expression of preproorexin and orexin receptors genes in human normal and prostate cancer cell lines

Visinin-like peptide 1 in adrenal gland of the rat. Gene expression and its hormonal control

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