Angiogenesis involvement by octacalcium phosphate-gelatin composite-driven bone regeneration in rat calvaria critical-sized defect

Kurobane, Tsuyoshi; Shiwaku, Yukari; Anada, Takahisa; Hamai, Ryo; Tsuchiya, Kaori; Baba, Kazuyoshi; Iikubo, Masahiro; Takahashi, Tsuneo; Сузукі, Осаму

doi:10.1016/j.actbio.2019.02.021

Cited by 53 publications

(42 citation statements)

References 63 publications

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“…Although considerable progress has been made towards employing OCP/Col in a clinical setting, the underlying mechanisms and the factors involved in the bone regeneration process induced by OCP/Col are yet to be fully elucidated; this is important for establishing the mass limitation in bone regeneration. Our previous study suggested that angiogenesis occurred earlier than the new bone formation induced by OCP/gelatin composite (Kurobane et al, ), but these results were insufficient to demonstrate the relationships among other bone formation markers and angiogenesis factors in OCP/Col treatment. Therefore, the present study investigated whether OCP/Col has positive effects on bone regeneration by evaluating the expression and activity of cells or molecules related to bone formation during the critical early phase of healing, by immunohistochemistry and enzyme histochemistry, in a rat calvarial bone defect model.…”

Section: Introductionmentioning

confidence: 89%

Octacalcium phosphate collagen composite stimulates the expression and activity of osteogenic factors to promote bone regeneration

Kouketsu

Matsui

Kawai

et al. 2019

J Tissue Eng Regen Med

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Objective: This study investigated the bone regenerative properties of an octacalcium phosphate collagen composite (OCP/Col) in a rat calvarial bone defect model.Design: An OCP/Col or β-tricalcium phosphate (β-TCP)/Col disk was implanted into the critical-sized calvarial defects and fixed 2 or 4 weeks later. The radiopacity of defects was examined after disk implantation by the radiographic examination and micro-computed tomography (μ-CT). Immunohistochemical and histochemical analyses were carried out to assess the bone matrix maturation, neovascularization, and osteoclast and osteoblast distribution in the neonatal bone.Results: Radiographic and μ-CT examination of the area of implanted OCP/Col indicated the newly formed bone and no difference from those of the original bone.Osteopontin, osteocalcin, Runt-related transcription factor 2, type 1 collagen, vascular endothelial growth factor, and alkaline phosphatase or tartrate-resistant acid phosphatase in the newly formed calvarial bone and the surrounding connective tissue were detected by immunohistochemistry and histochemistry. Biomarker expression was not significantly elevated at the defect site; the area of which was calculated by dividing the distance from the healthy bone margin or calvarium and dura mater surface. There was no difference in the expression of these biomarkers in the OCP/Col group at 2 and 4 weeks after surgery. In addition, the expression levels of all markers were higher in the OCP/Col group than in the β-TCP/Col group at 2 and 4 weeks after surgery. Conclusions:The OCP/Col as a bone regeneration material not only exhibits osteoconductive activity that is dependent on residual healthy bone tissue, but also has osteoinductive capacity, which promotes angiogenesis and osteogenic cell invasion from host tissue into the bone defect. ---This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Section: Introductionmentioning

confidence: 89%

Octacalcium phosphate collagen composite stimulates the expression and activity of osteogenic factors to promote bone regeneration

Kouketsu

Matsui

Kawai

et al. 2019

J Tissue Eng Regen Med

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“…This is because OCP incorporates Ca 2+ and releases Pi ions during the hydrolysis reaction 8) . The decrement or increment of these ions in the medium depends on the amount of OCP incubated 28,30,100) . Furthermore, the pH locally decreased at the area adjacent to the OCP surface of its granules in a Tris-HCl buffer containing F − , although the buffering effect occurs to maintain the pH under the physiological condition 101) .…”

Section: Ion Environment In Physiological Condition Regulated By Ca-psmentioning

confidence: 99%

“…It is widely accepted that the induction of angiogenesis in the bone defect is one of the essential processes for the success of bone regeneration by implanted biomaterials 123) . OCP can promote early angiogenesis and facilitate new bone formation in a rat calvarial defect model 100) . To clarify how OCP can promote angiogenesis in vitro, human umbilical vein endothelial cells (HUVECs) were cultured in the presence or absence of OCP granules.…”

Section: Vascular Endothelial Cellsmentioning

confidence: 99%

“…Bone formation capacity of OCP in calvaria and long bones 1. Bone regeneration by OCP compared with β-TCP and HA in mouse calvaria OCP activates the bone cellular responses and stimulates bone remodeling processes 7,25,[28][29][30]100,122) . In order to compare the bone formation ability of Ca-Ps, OCP, β-TCP, and HA granules were implanted in mouse calvarial defects 128) .…”

Section: Macrophagesmentioning

confidence: 99%

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Octacalcium phosphate bone substitute materials: Comparison between properties of biomaterials and other calcium phosphate materials

2020

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Octacalcium phosphate (OCP) is a material that can be converted to hydroxyapatite (HA) under physiological environments and is considered a mineral precursor to bone apatite crystals. The structure of OCP consists of apatite layers stacked alternately with hydrated layers, and closely resembles the structure of HA. The performance of OCP as a bone substitute differs from that of HA materials in terms of their osteoconductivity and biodegradability. OCP manifests a cellular phagocytic response through osteoclastlike cells similar to that exhibited by the biodegradable material β-tricalcium phosphate (β-TCP). The use of OCP for human cranial bone defects involves using its granule or composite form with one of the natural polymers, viz., the reconstituted collagen. This review article discusses the differences and similarities in these calcium phosphate (Ca-P)-based materials from the viewpoint of the structure and their material chemistry, and attempts to elucidate why CaP materials, particularly OCP, display unique osteoconductive property.

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“…39,40 The improvement of vascularization allows gas exchange and nutrient transport to osteoblast cells, which are needed for repair of large bone defects. 41,42 Moreover, angiogenesis may lead to the recruitment of stem cells to the injured site and their orientation to the osteoblastic lineage. 43 In this context, scaffolds play an important role, especially in adequate vascularization at the defect site and consequently in bone regeneration.…”

Section: Nanoscale Papermentioning

confidence: 99%

Nanocellulose/bioactive glass cryogels as scaffolds for bone regeneration

et al. 2019

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Angiogenesis involvement by octacalcium phosphate-gelatin composite-driven bone regeneration in rat calvaria critical-sized defect

Cited by 53 publications

References 63 publications

Octacalcium phosphate collagen composite stimulates the expression and activity of osteogenic factors to promote bone regeneration

Octacalcium phosphate collagen composite stimulates the expression and activity of osteogenic factors to promote bone regeneration

Octacalcium phosphate bone substitute materials: Comparison between properties of biomaterials and other calcium phosphate materials

Nanocellulose/bioactive glass cryogels as scaffolds for bone regeneration

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