Angiogenic activity of leptin in the chick embryo chorioallantoic membrane is in part mediated by endogenous fibroblast growth factor-2

Ribatti, Doménico; Nico, Beatrice; Belloni, A. S.; Vacca, Alessandra; Roncali, Luisa; Gg, Nussdorfer

doi:10.3892/ijmm.8.3.265

Cited by 38 publications

(38 citation statements)

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“…Our study of quail CAM under two experimental conditions clearly demonstrated that leptin enhanced the formation of new blood vessels. The results are in accordance with the data obtained using chicken CAM (Bouloumié et al 1998;Ribatti et al 2001). Bouloumié et al (1998) applied leptin at ED9 at higher doses (0.2, 1, and 3 µg of leptin) onto methylcellulose discs.…”

Section: Discussionsupporting

confidence: 82%

Angiogenic Effect of Leptin in the Quail Chorioallantoic Membrane

et al. 2010

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Leptin, the product of ob gene, beside its key role in the control of body weight and food consumption, can be involved in the control of embryonic development. Leptin administration in ovo accelerated the embryonic and post-embryonic development in Japanese quail. Although the mechanisms of leptin effects on growth and development acceleration are not clear, stimulation of angiogenesis represents one of plausible explanations. Therefore, the aim of the present study was to investigate the pro-angiogenic effect of leptin in vivo in the quail chorioallantoic membrane (CAM). The recombinant murine leptin (10, 100, and 1000 ng) was applied either ex ovo on the CAM surface of ex ovo incubated embryos at embryonic day 7 (ED7) or in ovo into the egg albumen at ED5. Changes in blood vessels were quantified by the fractal analysis providing the fractal dimension (Df) estimate. Leptin administered in ovo was more efficient in stimulation of angiogenesis than the ex ovo treatment, since 10 ng dose elicited significantly higher (P < 0.001) stimulation of vessel development of the CAM under the air cell than it did after ex ovo cultivation. Our study confirmed that exogenously applied leptin was able to stimulate angiogenesis in CAM. Leptin-mediated stimulation of angiogenesis may improve nutrient utilization from the yolk and explain at least partially the accelerating effect of leptin on avian embryo growth and development.

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Section: Discussionsupporting

confidence: 82%

Angiogenic Effect of Leptin in the Quail Chorioallantoic Membrane

et al. 2010

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“…Growth factor Esch et al (1985) Basic fibroblast growth factor (bFGF) or Wilting et al (1991) Fibroblast growth factor-2 (FGF-2) Olivo et al (1992) Ribatti et al (1995) Fett et al (1985 Angiogenin Wilting et al (1991) Olivo et al (1992 Leibovich et al (1987) Tumor necrosis factor alpha (TNF-␣) Olivo et al (1992) Yang andMoses (1990) Transforming growth factor beta (TGF-␤) Wilting et al (1992) Platelet derived growth factor (PDGF) Wilting et al (1992Wilting et al ( , 1993 Vascular endothelial growth factor (VEGF) Ziche et al (1997) Placenta growth factor-1 (PIGF-1) Bouloumie et al (1998) Leptin Ribatti et al (2001 Erythropoietin (EPO) Ramoshebi and Ripamonti (2000) Osteogenic protein-1 (OP-1) Bernardini et al (2000) CC chemokine I-309…”

Section: Authorsmentioning

confidence: 99%

Chorioallantoic membrane capillary bed: A useful target for studying angiogenesis and anti‐angiogenesis in vivo

et al. 2001

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The chick embryo chorioallantoic membrane (CAM) is an extraembryonic membrane that is commonly used in vivo to study both angiogenesis and anti-angiogenesis. This review 1) summarizes the current knowledge about the structure of the CAM's capillary bed; 2) discusses the controversy about the existence of a single blood sinus or a capillary plexus underlying the chorionic epithelium; 3) describes a new model of the CAM vascular growth, namely the intussusceptive mode; 4) reports findings regarding the role played by endogenous fibroblast growth factor-2 in CAM vascularization; and 5) addresses the use and limitations of the CAM as a model for studying angiogenesis and anti-angiogenesis. Anat Rec 264: [317][318][319][320][321][322][323][324] 2001.

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“…IFN-a reduces urokinase-type plasminogen activator and plasminogen activator inhibitor-1 activity (135) and significantly inhibits MMP-9 enzymatic activity and protein expression (136). IFN-a and IFN-h treatment also inhibits angiogenesis by down-regulation of bFGF expression (137)(138)(139), but other studies suggest that the action of IFN-a is not mediated by bFGF or VEGF (140)(141)(142).…”

Section: Non-matrix-derived Inhibitors Of Angiogenesismentioning

confidence: 99%