Angiogenic factors in relation to embryo implantation

Bagheri, Azadeh; Chianeh, Yousef Rezaei; Kumar, Pratap; Rao, Pragna

doi:10.5455/2320-1770.ijrcog20141201

Cited by 3 publications

(4 citation statements)

References 74 publications

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“…VEGF functions are mediated via binding to its tyrosine kinase receptors; VEGF receptor 1 (VEGFR1/Flt1) and VEGF receptor 2 (VEGFR2/Flk1/KDR). Expression of VEGF-A and VEGF-C has been demonstrated in the human endometrium throughout the menstrual cycle with an increase in the late proliferative and secretory phases (13,14). In addition, VEGF's expression was found in decidual cells of early pregnancy (15).…”

Section: Introductionmentioning

confidence: 93%

“…The previous study showed the role of VEGF-A and VEGF-C in implantation and placentation (28,29,30). Also, there is evidence of the role of VEGF in recurrent miscarriage (31,13).…”

Section: Discussionmentioning

confidence: 99%

“…In addition, VEGF's expression was found in decidual cells of early pregnancy (15). On the other hand, expression of VEGF receptors was shown in human endometrium (13).…”

Section: Introductionmentioning

confidence: 96%

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Association of angiogenic cytokines (VEGF-A and VEGF-C) and clinical characteristic in women with unexplained recurrent miscarriage

Bagheri¹,

Kumar²,

Kamath³

et al. 2017

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INTRODUCTION: Recurrent miscarriage (RM) defi ned as 2 or more spontaneous miscarriage before 20 weeks of gestation, affects at least 1 % of couples trying to conceive. In over 50 % of cases, the cause of the loss of pregnancy remains unexplained. Reduced expression of Angiogenic factors such as: vascular endothelial growth factor (VEGF-A) and VEGF-C has been linked with spontaneous miscarriage, likely due to defective foetal and placental angiogenesis. AIMS AND OBJECTIVES: To investigate the relationships between serum level of VEGF-A and VEGF-C with clinical characteristic in women with URM and compare to pregnant and healthy women. MATERIALS AND METHODS: A case-control study, which was conducted between 90 non-pregnant women with history of RM, age-matched with 70 non-pregnant women without history of recurrent abortion with at least one child (controls) and 70 pregnant women without history of recurrent abortion with at least one child (controls). Those with unexplained RM were eligible. Demographic and Anthropometric data were retrieved by pre-test questionnaire and serum level of VEGF-A and VEGF-C measured by ELISA kit. RESULTS: This study showeds that maternal levels of VEGF-A and VEGF-C were distinctly lower in RSA (189.87±88.1 vs 238.8±99.6) compared to healthy (239.1±99.7 vs 275.5±133.08) and pregnant (301.5±76.4 vs 402.5±128.6) women as control groups. Univariate analysis demonstrated that clinical characteristic factors were signifi cantly associated with concentration of VEGF-A and VEGF-C in cases and controls. CONCLUSIONS: Our fi ndings suggest that these molecules could be used as potential predictive markers of miscarriage in these women presenting with URM (Tab. 4, Fig. 5, Ref. 40). Text in PDF www.elis.sk.

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Section: Introductionmentioning

confidence: 93%

“…The previous study showed the role of VEGF-A and VEGF-C in implantation and placentation (28,29,30). Also, there is evidence of the role of VEGF in recurrent miscarriage (31,13).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Association of angiogenic cytokines (VEGF-A and VEGF-C) and clinical characteristic in women with unexplained recurrent miscarriage

Bagheri¹,

Kumar²,

Kamath³

et al. 2017

BLL

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“…Since angiogenesis is an important phenomenon during embryo implantation and is mediated mainly by VEGF (Zhang et al 2001, Wang & Dey 2006, Demir et al 2010, Bagheri et al 2017, we measured the expression of VEGF and its associated downstream effector molecules like PI3K, AKT and NOS that are known to be involved in the process of angiogenesis. Quantification of VEGF level was analyzed in fresh conditioned media collected 24 h after Attachment and expansion of BeWo trophoblast spheroids on RL95-2 cells.…”

Section: Sorcin Knock Down Significantly Reduced the Expression Of Vementioning

confidence: 99%

Sorcin is involved during embryo implantation via activating VEGF/PI3K/Akt pathway in mice

Gupta

Sirohi

Kumari

et al. 2018

Journal of Molecular Endocrinology

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Our earlier studies have demonstrated the cyclic variation and also the altered expression of sorcin in endometrium during early-to-mid-secretory phase transition in women with unexplained infertility. The current study was undertaken to establish the functional role of sorcin in endometrial receptivity in mice. Results indicated that sorcin was highly expressed during the window of implantation in mice and functional blockage of sorcin caused significant reduction in number of implanted blastocyst. The receptivity markers (i.e.Integrin β3, HBEGF, IGFBP1, WNT4 and Cyclin E)) were found to be downregulated in sorcin knocked down uterine horn on day 5 as compared to untreated horn. The reduced attachment and expansion of BeWo spheroids on RL95-2 endometrial cells with sorcin knock down, in model of endometrium-trophoblast interaction further supported these findings. Uterine sorcin expression pattern during estrous cycle and in delayed implantation mice model suggested the upregulation of sorcin by estrogen. The functional blockade of sorcin induced the intracellular Ca levels in endometrial epithelial cells (EECs), which indicated that altered Ca homeostasis might be responsible for implantation failure. Sorcin silencing led to significant reduction in the expression of angiogenic factor VEGF and its downstream effector molecules i.e. PI3K, Akt and NOS. The migratory and invasive properties of HUVECs were abrogated by anti-VEGF or by adding culture media from sorcin blocked EECs, which indicated that sorcin might mediate angiogenesis during implantation. Taken together, sorcin is involved in the regulation of Ca-mediated angiogenesis via VEGF/PI3K/Akt pathway in endometrial cells and plays a crucial role in preparing the endometrium for implantation.

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Physiology of Endometrial Development through the Cycle and Implantation

Brennan¹,

Hickey²

2019

How to Prepare the Endometrium to Maximize Implantation Rates and IVF Success

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The embryo transfer is the final step in the in vitro fertilization (IVF) treatment cycle, and it is the step with the greatest failure rate. Success requires implantation which depends on the normality of the embryo its ability to achieve apposition, adhesion and attachment. For this to occur successfully, endometrium has to be receptive. Endometrial receptivity is considered the 'Black Box' of reproductive technologies. While considerable advances have been made in obtaining oocytes, fertilization and embryo development in vitro, little progress has been achieved in improving implantation rates following transfer of a high-quality embryo. This will require a greater understanding of how the endometrium becomes receptive, the blastocyst-maternal interactions during the final stages of blastocyst development in the microenvironment within the uterine cavity, and how appropriate preparation of both the endometrial epithelium and the developing decidua are critical to establish a viable pregnancy. The contributions in this volume, from well-recognized experts in their fields, will update clinicians and scientists alike on the latest developments in the field.

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Angiogenic factors in relation to embryo implantation

Cited by 3 publications

References 74 publications

Association of angiogenic cytokines (VEGF-A and VEGF-C) and clinical characteristic in women with unexplained recurrent miscarriage

Association of angiogenic cytokines (VEGF-A and VEGF-C) and clinical characteristic in women with unexplained recurrent miscarriage

Sorcin is involved during embryo implantation via activating VEGF/PI3K/Akt pathway in mice

Physiology of Endometrial Development through the Cycle and Implantation

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