Angiography of Fluoresceinated Anti–Vascular Endothelial Growth Factor Antibody and Dextrans in Experimental Choroidal Neovascularization

Abstract

To determine if anti-vascular endothelial growth factor antibody and a range of dextrans with varying diffusion radii and molecular weights are permeable through experimental choroidal neovascularization (CNV). Methods: Choroidal neovascularization was induced in 10 cynomolgus monkey retinas by means of argon laser injury. Digital fundus fluorescein angiograms were performed with fluorescein sodium, fluoresceinated IgG antibodies (anti-vascular endothelial growth factor and a control antibody), and fluoresceinated dextrans with molecular weights of 4, 20, 40, 70 and 150 kd. The 40-and 70-kd dextrans straddle the effective diffusion radius of IgG. For each reagent, early and late angiograms were performed in a standardized fashion, with follow-up images obtained to monitor residual fluorescence. Results: Perfusion of retinal vessels and choroidal vasculature was seen with all reagents. Fluorescein and 4and 20-kd dextran leaked rapidly from the CNV within the first minute. Angiography with the use of 40-kd dextran and fluoresceinated antibody, either anti-vascular

Development of Ranibizumab, an Anti–vascular Endothelial Growth Factor Antigen Binding Fragment, as Therapy for Neovascular Age-Related Macular Degeneration

“…Direct experimental evidence that intravenously injected fluoresceinated bevacizumab or control IgG leaks from CNV in a primate model was then obtained. 84 However, the safety of prolonged systemic exposure to an anti-VEGF agent was a potential concern. Although toxicological studies of young adult monkeys receiving intravenously administered bevacizumab had not revealed any major safety signals, 85 the possibility that adverse effects might occur in an elderly population such as AMD patients, frequently affected by cardiovascular diseases, could not be ruled out.…”

Investigation of Laser-Induced Choroidal Neovascularization in the Rat

“…7,14 Advantages of dextran angiography examined in flatmounts are the representation of the whole retina and a higher resolution of certain regions, 7 as well as the possibility of quantification by measuring the fluorescence. Angiography is faster and easier to perform than histologic methods.…”

In Vivo Imaging of a New Indocyanine Green Micelle Formulation in an Animal Model of Laser-Induced Choroidal Neovascularization

“…[24][25][26][27][28][29] For example, imaging of real-time retinal cell apoptosis 25 and laser-induced CNV has been reported. [30][31][32][33][34] The aim of the current study was to develop a new formulation of ICG with better chemical stability and modified Copyright 2014 The Association for Research in Vision and Ophthalmology, Inc. www.iovs.org j ISSN: 1552-5783 fluorescence characteristics, and to test this molecule in vitro and in an animal model with in vivo and ex vivo correlations. The potentially better and longer visualization of this modified dye may be helpful for an improved detection and monitoring of pathological alterations in various eye diseases.…”