Angiography of Malignant Tumors in Mice

Margulis, Alexander R.; Carlsson, Erik; McAlister, William H.

doi:10.3109/00016926109172575

Cited by 29 publications

(7 citation statements)

References 5 publications

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“…Margulis et al 24 and Gullino and Granthamls found that flow remained the same; however, Cataland et al9 found a decrease. T h e estimated blood flow is inaccurate when the tumor is small because it includes that of a relatively large volume of healthy skin but, as the tumor enlarges, the overlying skin is stretched; its vasculature is decreased and heat flow from surrounding skin to the large tumor mass can be ignored.…”

Section: Resultsmentioning

confidence: 93%

“…T h e estimated blood flow is inaccurate when the tumor is small because it includes that of a relatively large volume of healthy skin but, as the tumor enlarges, the overlying skin is stretched; its vasculature is decreased and heat flow from surrounding skin to the large tumor mass can be ignored. Margulis et al 24 have shown that the blood supply of transplanted C3H tumors is the same as that of the spontaneous growths. This was confirmed by a "thermo-stromuhr,"g which directly measures the tumor heat conductivity which is proportional to blood flow, and by the use of a 4-thermistor "thermal circulation index" which corrects for flow in the underlying tissues and skin.23 Blood flow generally has been found to be less in malignant than normal tissues but views differ on the effect of an increase in tumor size.…”

Section: Resultsmentioning

confidence: 99%

“…This was confirmed by a "thermo-stromuhr,"g which directly measures the tumor heat conductivity which is proportional to blood flow, and by the use of a 4-thermistor "thermal circulation index" which corrects for flow in the underlying tissues and skin.23 Blood flow generally has been found to be less in malignant than normal tissues but views differ on the effect of an increase in tumor size. Margulis et al 24 and Gullino and Granthamls found that flow remained the same; however, Cataland et al9 found a decrease. Vogel30 reported that the total vascular volume remained constant but that the sinusoidal volume increased, producing a decrease in the vascular surface area.…”

Section: Methodsmentioning

confidence: 93%

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Blood flow and oxygenation of tumors in mice.I. Effects of breathing gases containing carbon dioxide at atmospheric pressure

Kruuv

Inch

McCredie

1967

Cancer

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Inhalation of 5% carbon dioxide (CO2) and 95% oxygen (O2) or 10% CO2 and 90% O2 at one atm pressure increased blood flow of the C3HBA isotransplant and C3H isoimplant in C3H mice compared with animals breathing 100% O2 or air. Oxygen concentration, measured by a platinum microelectrode, was improved in anoxic areas of the tumor when the animals inhaled the gases containing CO2 compared with those breathing 100% O2 or air; the relative increase was less when the electrode was placed in a well‐vascularized area. Rectal temperature, heart and respiration rates were decreased in animals breathing the gases containing CO2 compared with air; rectal temperature was increased by inhalation of 100% O2. The results suggest that inhalation of a gas mixture containing CO2 at atmospheric pressure during radiotherapy or chemotherapy may be of value in treating cancer in humans.

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Section: Resultsmentioning

confidence: 93%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 93%

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Blood flow and oxygenation of tumors in mice.I. Effects of breathing gases containing carbon dioxide at atmospheric pressure

Kruuv

Inch

McCredie

1967

Cancer

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“…Assumptions about changes in vascular structure are frequently based upon induced functional changes and vice versa. The use of contrast media, such as India ink (Lewis, 1927) and colloidal carbon (Hilmas & Gillette, 1973), or radio-opaque medium (Solesvik et al, 1984;Margulis et al, 1961), although providing elegant structural information about tumour vasculature, must necessarily neglect those vessels which may not be available to the contrast agent at the time of the experiment. Conversely those methods which aim to determine vascular volume, and other parameters, by purely histochemical means (Tannock & Steel, 1969;Revesz & Siracka, 1984) provide little information about the efJective functional vascular volume.…”

Section: Resultsmentioning

confidence: 99%

The effects of melphalan and misonidazole on the vasculature of a murine sarcoma

Jc¹,

Randhawa²,

Denekamp³

1987

Br J Cancer

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Summary A method for estimating both structural and functional vascular volumes in murine sarcomas is described. Intact vessels were demonstrated by the presence of laminin, a basement membrane-associated antigen, using an immunofluorescent technique. and functional vessels in the same sample by prior injection with the DNA binding dye Hoechst 33342. No significant vascular effects were seen after melphalan but a very pronounced decrease in both functional and structural vascular volume was seen after MISO. Combined chemotherapy of a murine sarcoma with melphalan and MISO induced a rapid decrease in the functional vascular volume, anid there was a resumption of blood flow prior to measurable regrowth. The fully regrown tumour retained the vascular characteristics of untreated tumours of similar size.The observed effects of ionizing radiation on tumour growth are attributed by some authors to a direct cytotoxic action on tumour cells, as well as an indirect cytotoxic action, resulting from vascular and stromal damage (Thomlinson & Craddock, 1966;Mattson & Peterson, 1979 Song, 1984). As occlusion of the vasculature can alone lead to tumour control or cure (Denekamp et al., 1983), the vascular component of any form of therapy must not be underestimated; indeed the vasculature itself may represent an important target for therapy (Denekamp, 1984). In contrast to radiation and hyperthermia, however, little is known about the possible contribution of a vascular component in chemotherapy; in general the effects are considered the result of direct tumour cell kill.In this study we have examined the effects of the alkylating agent melphalan on the vasculature of a murine sarcoma when used alone or in combination with the radiosensitizer Misonidazole (MISO). MISO is known to potentiate the effects of melphalan. The origins of this phenomenon, known as 'chemosensitization', are not fully understood. Among the possible mechanisms that have been proposed are: that MISO is directly cytotoxic to hypoxic cells, alters the pharmacokinetics of the cytotoxic drugs, interferes with the repair of potentially lethal damage, or depletes intracellular thiols; (For reviews see McNally, 1982;Millar, 1982;Siemann, 1982;. The present study was carried out to determine whether a vascular component could be attributed to the potentiation of melphalan cytotoxicity by MISO. Although many methods exist for studying structural and functional parameters of normal tissues and tumours, in general these methods do not distinguish patent vessels from those which may be temporarily, or permanently, non functional. We utilize here a method whereby, in the same tumour sample, structural and functional information can be obtained using a morphometric technique. The method relies upon the identification of blood vessels by the immunolluorescent demonstration of the glycoprotein laminin, present in the blood vessel walls (Timpl et al., 1979). This structural marker was used in parallel with a functional fluorescent marker, bis-benzamide Hoechst 33342, inj...

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“…Zur Untersuchung des Tumorgef~iBsystems sind Tiermodelle geeignet, da sie gleichbleibende und reproduzierbare Verh/iltnisse bieten und eine objektive Erfolgsbeurteilung nach therapeutischen Eingriffen zulassen [10,19]. Uber r6ntgenologisch-mikroangiographische Befunde von tierexperimentellen Tumoren nach progredientem Wachstum wurde von mehreren Untersuchern berichtet [1,2,13,17]. Meist standen jedoch nur einzelne Tumoren, vorwiegend mesenchymaler Herkunft, zur Verftingung.…”

Section: Introductionunclassified

Mikroangiographische Untersuchungen verschiedener menschlicher Tumoren nach Transplantation auf thymusaplastische Nacktmäuse

Kraus¹,

Fiebig

Schuchhardt

et al. 1983

Res. Exp. Med.

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Ten different human tumors (two soft tissue sarcomas, seven adenocarcinomas of the colon and stomach and, one squamous cell carcinoma of the lung) were implanted s.c. in thymus-aplastic mice and retransplanted over several generations. In 40 animals, the tumors were studied by microangiography and microscopy and classified according to angiographic tumor criteria. Tumor transplants always revealed similar angiographic patterns. The microscopic results presented a good correlation with the roentgenologic findings. The microstructure of the tumor vessels and lacunes are analyzed and discussed. The nude mice model is well suitable for the research of human tumors.

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Angiography of Malignant Tumors in Mice

Cited by 29 publications

References 5 publications

Blood flow and oxygenation of tumors in mice.I. Effects of breathing gases containing carbon dioxide at atmospheric pressure

Blood flow and oxygenation of tumors in mice.I. Effects of breathing gases containing carbon dioxide at atmospheric pressure

The effects of melphalan and misonidazole on the vasculature of a murine sarcoma

Mikroangiographische Untersuchungen verschiedener menschlicher Tumoren nach Transplantation auf thymusaplastische Nacktmäuse

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